Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1356

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhB and flhA are separated by 118 nucleotidesflhA and flhF are separated by 11 nucleotides Shewana3_1355: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 1,566,609 to 1,567,739 flhB Shewana3_1356: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 1,567,858 to 1,569,957 flhA Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345 flhF Position (kb) 1567 1568 1569 1570Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 1567.313 kb on + strand, within flhBat 1567.415 kb on + strand, within flhBat 1567.429 kb on - strand, within flhBat 1567.478 kb on + strand, within flhBat 1567.605 kb on + strand, within flhBat 1567.681 kb on + strandat 1567.702 kb on - strandat 1567.881 kb on - strandat 1567.882 kb on - strandat 1567.939 kb on - strandat 1568.056 kb on + strandat 1568.064 kb on - strandat 1568.064 kb on - strandat 1568.064 kb on - strandat 1568.064 kb on - strandat 1568.426 kb on + strand, within flhAat 1568.505 kb on + strand, within flhAat 1568.549 kb on + strand, within flhAat 1569.001 kb on + strand, within flhAat 1569.099 kb on + strand, within flhAat 1569.213 kb on + strand, within flhAat 1569.221 kb on + strand, within flhAat 1569.269 kb on + strand, within flhAat 1569.277 kb on - strand, within flhAat 1569.277 kb on - strand, within flhAat 1569.277 kb on - strand, within flhAat 1569.345 kb on - strand, within flhAat 1570.008 kb on + strandat 1570.180 kb on + strand, within flhFat 1570.239 kb on + strand, within flhFat 1570.329 kb on + strand, within flhFat 1570.332 kb on + strand, within flhFat 1570.349 kb on + strand, within flhFat 1570.690 kb on + strand, within flhFat 1570.767 kb on + strand, within flhFat 1570.878 kb on + strand, within flhF

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,567,313 + flhB Shewana3_1355 0.62 +0.5
1,567,415 + flhB Shewana3_1355 0.71 -1.3
1,567,429 - flhB Shewana3_1355 0.73 +1.5
1,567,478 + flhB Shewana3_1355 0.77 -0.1
1,567,605 + flhB Shewana3_1355 0.88 -1.5
1,567,681 + +1.5
1,567,702 - -2.0
1,567,881 - +1.5
1,567,882 - -0.1
1,567,939 - +1.5
1,568,056 + -3.0
1,568,064 - +0.5
1,568,064 - +1.5
1,568,064 - +1.5
1,568,064 - +1.5
1,568,426 + flhA Shewana3_1356 0.27 +1.5
1,568,505 + flhA Shewana3_1356 0.31 -3.3
1,568,549 + flhA Shewana3_1356 0.33 -3.7
1,569,001 + flhA Shewana3_1356 0.54 -6.1
1,569,099 + flhA Shewana3_1356 0.59 -6.1
1,569,213 + flhA Shewana3_1356 0.65 -3.9
1,569,221 + flhA Shewana3_1356 0.65 -3.5
1,569,269 + flhA Shewana3_1356 0.67 -3.4
1,569,277 - flhA Shewana3_1356 0.68 +1.5
1,569,277 - flhA Shewana3_1356 0.68 +0.5
1,569,277 - flhA Shewana3_1356 0.68 +1.5
1,569,345 - flhA Shewana3_1356 0.71 -1.1
1,570,008 + -5.1
1,570,180 + flhF Shewana3_1357 0.15 -5.0
1,570,239 + flhF Shewana3_1357 0.20 -8.1
1,570,329 + flhF Shewana3_1357 0.26 -3.0
1,570,332 + flhF Shewana3_1357 0.26 -2.2
1,570,349 + flhF Shewana3_1357 0.28 -4.0
1,570,690 + flhF Shewana3_1357 0.52 -7.1
1,570,767 + flhF Shewana3_1357 0.58 -4.4
1,570,878 + flhF Shewana3_1357 0.66 -4.3

Or see this region's nucleotide sequence