Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhB and flhA are separated by 118 nucleotides flhA and flhF are separated by 11 nucleotides
Shewana3_1355: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 1,566,609 to 1,567,739
flhB
Shewana3_1356: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 1,567,858 to 1,569,957
flhA
Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345
flhF
Position (kb)
1567
1568
1569
1570 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1567.313 kb on + strand, within flhB at 1567.415 kb on + strand, within flhB at 1567.429 kb on - strand, within flhB at 1567.478 kb on + strand, within flhB at 1567.605 kb on + strand, within flhB at 1567.681 kb on + strand at 1567.702 kb on - strand at 1567.881 kb on - strand at 1567.882 kb on - strand at 1567.939 kb on - strand at 1568.056 kb on + strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.426 kb on + strand, within flhA at 1568.505 kb on + strand, within flhA at 1568.549 kb on + strand, within flhA at 1569.001 kb on + strand, within flhA at 1569.099 kb on + strand, within flhA at 1569.213 kb on + strand, within flhA at 1569.221 kb on + strand, within flhA at 1569.269 kb on + strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.345 kb on - strand, within flhA at 1570.008 kb on + strand at 1570.180 kb on + strand, within flhF at 1570.239 kb on + strand, within flhF at 1570.329 kb on + strand, within flhF at 1570.332 kb on + strand, within flhF at 1570.349 kb on + strand, within flhF at 1570.690 kb on + strand, within flhF at 1570.767 kb on + strand, within flhF at 1570.878 kb on + strand, within flhF
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,567,313 + flhB Shewana3_1355 0.62 +0.5 1,567,415 + flhB Shewana3_1355 0.71 -1.3 1,567,429 - flhB Shewana3_1355 0.73 +1.5 1,567,478 + flhB Shewana3_1355 0.77 -0.1 1,567,605 + flhB Shewana3_1355 0.88 -1.5 1,567,681 + +1.5 1,567,702 - -2.0 1,567,881 - +1.5 1,567,882 - -0.1 1,567,939 - +1.5 1,568,056 + -3.0 1,568,064 - +0.5 1,568,064 - +1.5 1,568,064 - +1.5 1,568,064 - +1.5 1,568,426 + flhA Shewana3_1356 0.27 +1.5 1,568,505 + flhA Shewana3_1356 0.31 -3.3 1,568,549 + flhA Shewana3_1356 0.33 -3.7 1,569,001 + flhA Shewana3_1356 0.54 -6.1 1,569,099 + flhA Shewana3_1356 0.59 -6.1 1,569,213 + flhA Shewana3_1356 0.65 -3.9 1,569,221 + flhA Shewana3_1356 0.65 -3.5 1,569,269 + flhA Shewana3_1356 0.67 -3.4 1,569,277 - flhA Shewana3_1356 0.68 +1.5 1,569,277 - flhA Shewana3_1356 0.68 +0.5 1,569,277 - flhA Shewana3_1356 0.68 +1.5 1,569,345 - flhA Shewana3_1356 0.71 -1.1 1,570,008 + -5.1 1,570,180 + flhF Shewana3_1357 0.15 -5.0 1,570,239 + flhF Shewana3_1357 0.20 -8.1 1,570,329 + flhF Shewana3_1357 0.26 -3.0 1,570,332 + flhF Shewana3_1357 0.26 -2.2 1,570,349 + flhF Shewana3_1357 0.28 -4.0 1,570,690 + flhF Shewana3_1357 0.52 -7.1 1,570,767 + flhF Shewana3_1357 0.58 -4.4 1,570,878 + flhF Shewana3_1357 0.66 -4.3
Or see this region's nucleotide sequence