Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1341

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1340 and Shewana3_1341 are separated by 175 nucleotidesShewana3_1341 and fliF are separated by 44 nucleotides Shewana3_1340: Shewana3_1340 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,553,017 to 1,554,357 _1340 Shewana3_1341: Shewana3_1341 - flagellar hook-basal body complex subunit FliE (RefSeq), at 1,554,533 to 1,554,871 _1341 Shewana3_1342: fliF - flagellar MS-ring protein (RefSeq), at 1,554,916 to 1,556,601 fliF Position (kb) 1554 1555Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3at 1553.560 kb on + strand, within Shewana3_1340at 1553.713 kb on + strand, within Shewana3_1340at 1553.727 kb on + strand, within Shewana3_1340at 1553.823 kb on - strand, within Shewana3_1340at 1554.068 kb on + strand, within Shewana3_1340at 1554.171 kb on + strand, within Shewana3_1340at 1554.171 kb on + strand, within Shewana3_1340at 1554.212 kb on - strand, within Shewana3_1340at 1554.302 kb on + strandat 1554.302 kb on + strandat 1554.308 kb on + strandat 1554.310 kb on - strandat 1554.417 kb on + strandat 1554.417 kb on + strandat 1554.417 kb on + strandat 1554.661 kb on - strand, within Shewana3_1341at 1554.810 kb on + strand, within Shewana3_1341at 1554.818 kb on - strand, within Shewana3_1341at 1554.856 kb on - strandat 1554.866 kb on + strandat 1554.953 kb on + strandat 1555.245 kb on + strand, within fliFat 1555.295 kb on + strand, within fliFat 1555.462 kb on + strand, within fliFat 1555.703 kb on + strand, within fliF

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,553,560 + Shewana3_1340 0.40 +0.4
1,553,713 + Shewana3_1340 0.52 -0.7
1,553,727 + Shewana3_1340 0.53 -1.2
1,553,823 - Shewana3_1340 0.60 +1.5
1,554,068 + Shewana3_1340 0.78 -1.7
1,554,171 + Shewana3_1340 0.86 -0.7
1,554,171 + Shewana3_1340 0.86 +0.3
1,554,212 - Shewana3_1340 0.89 +3.5
1,554,302 + -1.3
1,554,302 + -2.6
1,554,308 + -1.6
1,554,310 - -0.1
1,554,417 + -0.8
1,554,417 + -0.4
1,554,417 + -1.3
1,554,661 - Shewana3_1341 0.38 +0.5
1,554,810 + Shewana3_1341 0.82 -3.7
1,554,818 - Shewana3_1341 0.84 +1.5
1,554,856 - +1.5
1,554,866 + +1.5
1,554,953 + -4.3
1,555,245 + fliF Shewana3_1342 0.20 -2.6
1,555,295 + fliF Shewana3_1342 0.22 -2.3
1,555,462 + fliF Shewana3_1342 0.32 -6.3
1,555,703 + fliF Shewana3_1342 0.47 -2.1

Or see this region's nucleotide sequence