Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1313 and Shewana3_1314 are separated by 239 nucleotides Shewana3_1314 and Shewana3_1315 overlap by 4 nucleotides Shewana3_1315 and Shewana3_1316 are separated by 102 nucleotides Shewana3_1316 and Shewana3_1317 are separated by 2 nucleotides
Shewana3_1313: Shewana3_1313 - hypothetical protein (RefSeq), at 1,528,145 to 1,529,311
_1313
Shewana3_1314: Shewana3_1314 - hypothetical protein (RefSeq), at 1,529,551 to 1,530,183
_1314
Shewana3_1315: Shewana3_1315 - hypothetical protein (RefSeq), at 1,530,180 to 1,530,629
_1315
Shewana3_1316: Shewana3_1316 - FlgN family protein (RefSeq), at 1,530,732 to 1,531,157
_1316
Shewana3_1317: Shewana3_1317 - anti-sigma-28 factor, FlgM (RefSeq), at 1,531,160 to 1,531,486
_1317
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1528.620 kb on + strand, within Shewana3_1313 at 1528.892 kb on + strand, within Shewana3_1313 at 1529.000 kb on - strand, within Shewana3_1313 at 1529.321 kb on - strand at 1529.369 kb on - strand at 1529.420 kb on + strand at 1529.438 kb on + strand at 1529.473 kb on + strand at 1529.473 kb on + strand at 1529.481 kb on - strand at 1529.554 kb on - strand at 1529.561 kb on - strand at 1529.592 kb on + strand at 1529.715 kb on + strand, within Shewana3_1314 at 1529.751 kb on + strand, within Shewana3_1314 at 1529.959 kb on - strand, within Shewana3_1314 at 1530.092 kb on + strand, within Shewana3_1314 at 1530.202 kb on - strand at 1530.472 kb on - strand, within Shewana3_1315 at 1530.496 kb on + strand, within Shewana3_1315 at 1530.593 kb on - strand at 1530.638 kb on - strand at 1530.658 kb on + strand at 1531.098 kb on - strand, within Shewana3_1316 at 1531.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,528,620 + Shewana3_1313 0.41 -1.5 1,528,892 + Shewana3_1313 0.64 -3.6 1,529,000 - Shewana3_1313 0.73 +1.5 1,529,321 - +0.5 1,529,369 - +1.5 1,529,420 + -0.6 1,529,438 + +0.7 1,529,473 + -1.7 1,529,473 + -0.2 1,529,481 - -0.8 1,529,554 - +0.5 1,529,561 - +1.5 1,529,592 + -3.4 1,529,715 + Shewana3_1314 0.26 -4.4 1,529,751 + Shewana3_1314 0.32 -2.0 1,529,959 - Shewana3_1314 0.64 +1.5 1,530,092 + Shewana3_1314 0.85 -3.2 1,530,202 - +1.5 1,530,472 - Shewana3_1315 0.65 +1.5 1,530,496 + Shewana3_1315 0.70 -2.9 1,530,593 - +1.5 1,530,638 - +1.5 1,530,658 + -2.4 1,531,098 - Shewana3_1316 0.86 -4.9 1,531,133 - -6.7
Or see this region's nucleotide sequence