Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1213 and Shewana3_1214 are separated by 136 nucleotides Shewana3_1214 and Shewana3_1215 overlap by 41 nucleotides
Shewana3_1213: Shewana3_1213 - extracellular solute-binding protein (RefSeq), at 1,417,962 to 1,419,455
_1213
Shewana3_1214: Shewana3_1214 - CopG family transcriptional regulator (RefSeq), at 1,419,592 to 1,420,074
_1214
Shewana3_1215: Shewana3_1215 - hypothetical protein (RefSeq), at 1,420,034 to 1,420,816
_1215
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1418.624 kb on + strand, within Shewana3_1213 at 1418.677 kb on - strand, within Shewana3_1213 at 1418.891 kb on - strand, within Shewana3_1213 at 1418.907 kb on - strand, within Shewana3_1213 at 1419.204 kb on - strand, within Shewana3_1213 at 1419.592 kb on - strand at 1419.644 kb on + strand, within Shewana3_1214 at 1419.652 kb on - strand, within Shewana3_1214 at 1419.809 kb on + strand, within Shewana3_1214 at 1419.945 kb on + strand, within Shewana3_1214 at 1420.031 kb on + strand at 1420.076 kb on - strand at 1420.148 kb on + strand, within Shewana3_1215 at 1420.151 kb on - strand, within Shewana3_1215 at 1420.221 kb on + strand, within Shewana3_1215 at 1420.348 kb on + strand, within Shewana3_1215 at 1420.411 kb on + strand, within Shewana3_1215 at 1420.585 kb on + strand, within Shewana3_1215 at 1420.593 kb on - strand, within Shewana3_1215 at 1420.648 kb on + strand, within Shewana3_1215 at 1420.832 kb on + strand at 1420.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,418,624 + Shewana3_1213 0.44 -1.3 1,418,677 - Shewana3_1213 0.48 -0.1 1,418,891 - Shewana3_1213 0.62 +1.5 1,418,907 - Shewana3_1213 0.63 -1.9 1,419,204 - Shewana3_1213 0.83 +1.5 1,419,592 - +1.5 1,419,644 + Shewana3_1214 0.11 -0.3 1,419,652 - Shewana3_1214 0.12 +1.2 1,419,809 + Shewana3_1214 0.45 +0.2 1,419,945 + Shewana3_1214 0.73 +0.5 1,420,031 + -0.8 1,420,076 - +2.0 1,420,148 + Shewana3_1215 0.15 +0.5 1,420,151 - Shewana3_1215 0.15 -0.5 1,420,221 + Shewana3_1215 0.24 +1.2 1,420,348 + Shewana3_1215 0.40 +0.5 1,420,411 + Shewana3_1215 0.48 -1.1 1,420,585 + Shewana3_1215 0.70 +0.5 1,420,593 - Shewana3_1215 0.71 +1.3 1,420,648 + Shewana3_1215 0.78 -0.1 1,420,832 + -1.1 1,420,861 - -1.1
Or see this region's nucleotide sequence