Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0721 and Shewana3_0722 are separated by 34 nucleotides Shewana3_0722 and Shewana3_0723 are separated by 11 nucleotides Shewana3_0723 and Shewana3_0724 are separated by 44 nucleotides Shewana3_0724 and tolC are separated by 72 nucleotides
Shewana3_0721: Shewana3_0721 - enoyl-CoA hydratase (RefSeq), at 838,482 to 839,219
_0721
Shewana3_0722: Shewana3_0722 - hypothetical protein (RefSeq), at 839,254 to 839,736
_0722
Shewana3_0723: Shewana3_0723 - hypothetical protein (RefSeq), at 839,748 to 840,716
_0723
Shewana3_0724: Shewana3_0724 - hypothetical protein (RefSeq), at 840,761 to 841,456
_0724
Shewana3_0725: tolC - outer membrane channel protein (RefSeq), at 841,529 to 842,836
tolC
Position (kb) 839
840
841 Strain fitness (log2 ratio) -2
-1
0
1 at 838.794 kb on + strand, within Shewana3_0721 at 838.796 kb on - strand, within Shewana3_0721 at 838.802 kb on - strand, within Shewana3_0721 at 838.880 kb on - strand, within Shewana3_0721 at 838.890 kb on - strand, within Shewana3_0721 at 838.896 kb on - strand, within Shewana3_0721 at 838.900 kb on - strand, within Shewana3_0721 at 838.905 kb on - strand, within Shewana3_0721 at 839.207 kb on + strand at 839.228 kb on - strand at 839.267 kb on - strand at 839.381 kb on - strand, within Shewana3_0722 at 839.522 kb on + strand, within Shewana3_0722 at 839.909 kb on + strand, within Shewana3_0723 at 840.149 kb on + strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.272 kb on + strand, within Shewana3_0723 at 840.549 kb on - strand, within Shewana3_0723 at 840.593 kb on - strand, within Shewana3_0723 at 840.615 kb on + strand, within Shewana3_0723 at 840.675 kb on + strand at 840.746 kb on + strand at 840.746 kb on + strand at 840.754 kb on - strand at 840.890 kb on + strand, within Shewana3_0724 at 840.995 kb on + strand, within Shewana3_0724 at 841.054 kb on - strand, within Shewana3_0724 at 841.072 kb on + strand, within Shewana3_0724 at 841.092 kb on + strand, within Shewana3_0724 at 841.121 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.313 kb on + strand, within Shewana3_0724 at 841.671 kb on - strand, within tolC at 841.671 kb on - strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 838,794 + Shewana3_0721 0.42 -0.4 838,796 - Shewana3_0721 0.43 -0.4 838,802 - Shewana3_0721 0.43 +1.6 838,880 - Shewana3_0721 0.54 +0.5 838,890 - Shewana3_0721 0.55 +0.2 838,896 - Shewana3_0721 0.56 +0.2 838,900 - Shewana3_0721 0.57 -1.9 838,905 - Shewana3_0721 0.57 -0.9 839,207 + -1.0 839,228 - -1.3 839,267 - +0.3 839,381 - Shewana3_0722 0.26 +0.2 839,522 + Shewana3_0722 0.55 +0.9 839,909 + Shewana3_0723 0.17 -0.2 840,149 + Shewana3_0723 0.41 +1.6 840,186 - Shewana3_0723 0.45 +0.6 840,186 - Shewana3_0723 0.45 +1.6 840,272 + Shewana3_0723 0.54 -0.1 840,549 - Shewana3_0723 0.83 +1.6 840,593 - Shewana3_0723 0.87 +1.6 840,615 + Shewana3_0723 0.89 -0.8 840,675 + +0.4 840,746 + -0.5 840,746 + -0.7 840,754 - +1.6 840,890 + Shewana3_0724 0.19 -0.2 840,995 + Shewana3_0724 0.34 +0.0 841,054 - Shewana3_0724 0.42 +1.6 841,072 + Shewana3_0724 0.45 -0.2 841,092 + Shewana3_0724 0.48 -0.5 841,121 + Shewana3_0724 0.52 -0.3 841,220 + Shewana3_0724 0.66 +0.0 841,220 + Shewana3_0724 0.66 -2.5 841,313 + Shewana3_0724 0.79 -1.9 841,671 - tolC Shewana3_0725 0.11 -1.1 841,671 - tolC Shewana3_0725 0.11 -0.8
Or see this region's nucleotide sequence