Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplM and rpsI are separated by 14 nucleotides rpsI and Shewana3_0694 are separated by 113 nucleotides Shewana3_0694 and Shewana3_0695 are separated by 66 nucleotides Shewana3_0695 and Shewana3_0696 are separated by 108 nucleotides Shewana3_0696 and Shewana3_0697 are separated by 39 nucleotides
Shewana3_0692: rplM - 50S ribosomal protein L13 (RefSeq), at 809,168 to 809,596
rplM
Shewana3_0693: rpsI - 30S ribosomal protein S9 (RefSeq), at 809,611 to 810,003
rpsI
Shewana3_0694: Shewana3_0694 - hypothetical protein (RefSeq), at 810,117 to 810,305
_0694
Shewana3_0695: Shewana3_0695 - adenylosuccinate synthetase (RefSeq), at 810,372 to 811,667
_0695
Shewana3_0696: Shewana3_0696 - Sel1 domain-containing protein (RefSeq), at 811,776 to 812,432
_0696
Shewana3_0697: Shewana3_0697 - RNAse R (RefSeq), at 812,472 to 814,901
_0697
Position (kb) 810
811
812 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 810.099 kb on + strand at 810.121 kb on + strand at 810.248 kb on - strand, within Shewana3_0694 at 810.279 kb on - strand, within Shewana3_0694 at 810.390 kb on + strand at 810.689 kb on + strand, within Shewana3_0695 at 810.706 kb on + strand, within Shewana3_0695 at 810.716 kb on - strand, within Shewana3_0695 at 810.788 kb on + strand, within Shewana3_0695 at 810.827 kb on + strand, within Shewana3_0695 at 811.278 kb on + strand, within Shewana3_0695 at 811.365 kb on + strand, within Shewana3_0695 at 811.465 kb on + strand, within Shewana3_0695 at 811.534 kb on + strand, within Shewana3_0695 at 811.628 kb on + strand at 811.958 kb on - strand, within Shewana3_0696 at 812.042 kb on + strand, within Shewana3_0696 at 812.051 kb on - strand, within Shewana3_0696 at 812.078 kb on - strand, within Shewana3_0696 at 812.262 kb on + strand, within Shewana3_0696 at 812.507 kb on - strand at 812.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 810,099 + -0.2 810,121 + -1.1 810,248 - Shewana3_0694 0.69 -1.0 810,279 - Shewana3_0694 0.86 +0.9 810,390 + -0.0 810,689 + Shewana3_0695 0.24 -1.0 810,706 + Shewana3_0695 0.26 -0.2 810,716 - Shewana3_0695 0.27 +1.6 810,788 + Shewana3_0695 0.32 -2.8 810,827 + Shewana3_0695 0.35 -2.4 811,278 + Shewana3_0695 0.70 -1.2 811,365 + Shewana3_0695 0.77 +0.4 811,465 + Shewana3_0695 0.84 -1.2 811,534 + Shewana3_0695 0.90 -1.4 811,628 + +0.4 811,958 - Shewana3_0696 0.28 -4.0 812,042 + Shewana3_0696 0.40 -2.1 812,051 - Shewana3_0696 0.42 -3.2 812,078 - Shewana3_0696 0.46 -0.7 812,262 + Shewana3_0696 0.74 -5.3 812,507 - -0.2 812,644 + -0.6
Or see this region's nucleotide sequence