Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0560 and Shewana3_0561 are separated by 79 nucleotides Shewana3_0561 and Shewana3_0562 are separated by 221 nucleotides Shewana3_0562 and Shewana3_0563 are separated by 80 nucleotides
Shewana3_0560: Shewana3_0560 - formate--tetrahydrofolate ligase (RefSeq), at 653,188 to 654,900
_0560
Shewana3_0561: Shewana3_0561 - MscS mechanosensitive ion channel (RefSeq), at 654,980 to 656,635
_0561
Shewana3_0562: Shewana3_0562 - hypothetical protein (RefSeq), at 656,857 to 657,186
_0562
Shewana3_0563: Shewana3_0563 - hypothetical protein (RefSeq), at 657,267 to 657,392
_0563
Position (kb) 654
655
656
657 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 654.144 kb on - strand, within Shewana3_0560 at 654.215 kb on + strand, within Shewana3_0560 at 654.215 kb on + strand, within Shewana3_0560 at 654.493 kb on - strand, within Shewana3_0560 at 654.527 kb on + strand, within Shewana3_0560 at 654.537 kb on + strand, within Shewana3_0560 at 654.590 kb on - strand, within Shewana3_0560 at 654.604 kb on + strand, within Shewana3_0560 at 654.718 kb on - strand, within Shewana3_0560 at 654.812 kb on - strand at 654.812 kb on - strand at 654.844 kb on - strand at 654.849 kb on - strand at 655.186 kb on + strand, within Shewana3_0561 at 655.204 kb on - strand, within Shewana3_0561 at 655.285 kb on - strand, within Shewana3_0561 at 655.328 kb on + strand, within Shewana3_0561 at 655.731 kb on - strand, within Shewana3_0561 at 655.759 kb on - strand, within Shewana3_0561 at 656.233 kb on - strand, within Shewana3_0561 at 656.672 kb on - strand at 656.696 kb on + strand at 656.750 kb on - strand at 656.972 kb on + strand, within Shewana3_0562 at 656.980 kb on - strand, within Shewana3_0562 at 657.033 kb on - strand, within Shewana3_0562 at 657.227 kb on - strand at 657.254 kb on + strand at 657.254 kb on + strand at 657.262 kb on - strand at 657.262 kb on - strand at 657.398 kb on - strand at 657.418 kb on - strand at 657.437 kb on + strand at 657.437 kb on + strand at 657.437 kb on + strand at 657.445 kb on - strand at 657.445 kb on - strand at 657.445 kb on - strand at 657.447 kb on + strand at 657.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 654,144 - Shewana3_0560 0.56 -0.2 654,215 + Shewana3_0560 0.60 -1.5 654,215 + Shewana3_0560 0.60 -0.3 654,493 - Shewana3_0560 0.76 +1.4 654,527 + Shewana3_0560 0.78 +0.4 654,537 + Shewana3_0560 0.79 -0.6 654,590 - Shewana3_0560 0.82 +0.5 654,604 + Shewana3_0560 0.83 -0.5 654,718 - Shewana3_0560 0.89 -1.5 654,812 - +0.4 654,812 - -0.6 654,844 - -3.1 654,849 - -0.6 655,186 + Shewana3_0561 0.12 +0.7 655,204 - Shewana3_0561 0.14 -0.2 655,285 - Shewana3_0561 0.18 +0.3 655,328 + Shewana3_0561 0.21 +1.1 655,731 - Shewana3_0561 0.45 -0.3 655,759 - Shewana3_0561 0.47 +0.2 656,233 - Shewana3_0561 0.76 -1.2 656,672 - +1.6 656,696 + -0.0 656,750 - +0.0 656,972 + Shewana3_0562 0.35 +0.6 656,980 - Shewana3_0562 0.37 -2.3 657,033 - Shewana3_0562 0.53 +0.4 657,227 - -0.4 657,254 + +0.4 657,254 + +1.6 657,262 - -0.2 657,262 - -0.7 657,398 - -2.6 657,418 - -3.1 657,437 + +1.0 657,437 + +1.0 657,437 + -2.3 657,445 - +0.6 657,445 - -1.3 657,445 - -1.3 657,447 + -2.0 657,615 - -2.5
Or see this region's nucleotide sequence