Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0518 and Shewana3_0519 are separated by 129 nucleotides Shewana3_0519 and mscL are separated by 82 nucleotides mscL and Shewana3_0521 are separated by 184 nucleotides
Shewana3_0518: Shewana3_0518 - CzcA family heavy metal efflux protein (RefSeq), at 607,495 to 610,707
_0518
Shewana3_0519: Shewana3_0519 - antibiotic biosynthesis monooxygenase (RefSeq), at 610,837 to 611,160
_0519
Shewana3_0520: mscL - large-conductance mechanosensitive channel (RefSeq), at 611,243 to 611,653
mscL
Shewana3_0521: Shewana3_0521 - LysR family transcriptional regulator (RefSeq), at 611,838 to 612,791
_0521
Position (kb) 610
611
612 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 609.928 kb on + strand, within Shewana3_0518 at 609.938 kb on + strand, within Shewana3_0518 at 610.010 kb on + strand, within Shewana3_0518 at 610.054 kb on + strand, within Shewana3_0518 at 610.062 kb on - strand, within Shewana3_0518 at 610.085 kb on - strand, within Shewana3_0518 at 610.163 kb on + strand, within Shewana3_0518 at 610.233 kb on - strand, within Shewana3_0518 at 610.268 kb on + strand, within Shewana3_0518 at 610.439 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.560 kb on - strand at 610.568 kb on + strand at 610.574 kb on - strand at 610.632 kb on - strand at 610.639 kb on + strand at 610.647 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.743 kb on + strand at 610.930 kb on + strand, within Shewana3_0519 at 610.984 kb on + strand, within Shewana3_0519 at 611.031 kb on - strand, within Shewana3_0519 at 611.119 kb on + strand, within Shewana3_0519 at 611.127 kb on - strand, within Shewana3_0519 at 611.140 kb on + strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.464 kb on - strand, within mscL at 611.512 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.636 kb on - strand at 611.911 kb on + strand at 611.911 kb on + strand at 611.911 kb on + strand at 611.929 kb on - strand at 611.961 kb on + strand, within Shewana3_0521 at 612.003 kb on - strand, within Shewana3_0521 at 612.143 kb on + strand, within Shewana3_0521
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 609,928 + Shewana3_0518 0.76 -0.1 609,938 + Shewana3_0518 0.76 +0.3 610,010 + Shewana3_0518 0.78 -0.2 610,054 + Shewana3_0518 0.80 -1.8 610,062 - Shewana3_0518 0.80 +0.5 610,085 - Shewana3_0518 0.81 +0.4 610,163 + Shewana3_0518 0.83 -2.9 610,233 - Shewana3_0518 0.85 +1.6 610,268 + Shewana3_0518 0.86 +0.0 610,439 + +0.3 610,528 + -0.3 610,528 + -0.6 610,528 + -1.0 610,560 - -1.2 610,568 + +0.1 610,574 - -2.1 610,632 - +0.0 610,639 + -0.4 610,647 - +0.1 610,710 - -0.4 610,710 - +0.2 610,710 - -0.1 610,743 + -0.5 610,930 + Shewana3_0519 0.29 +0.1 610,984 + Shewana3_0519 0.45 -0.3 611,031 - Shewana3_0519 0.60 +1.8 611,119 + Shewana3_0519 0.87 -0.1 611,127 - Shewana3_0519 0.90 +0.2 611,140 + -1.1 611,267 - -0.9 611,267 - -0.7 611,267 - -0.9 611,464 - mscL Shewana3_0520 0.54 +0.1 611,512 - mscL Shewana3_0520 0.65 -0.0 611,557 - mscL Shewana3_0520 0.76 +0.2 611,557 - mscL Shewana3_0520 0.76 -2.2 611,636 - -1.9 611,911 + +2.4 611,911 + -1.2 611,911 + -1.2 611,929 - +0.1 611,961 + Shewana3_0521 0.13 -0.4 612,003 - Shewana3_0521 0.17 -2.4 612,143 + Shewana3_0521 0.32 +0.8
Or see this region's nucleotide sequence