Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0443 and Shewana3_0444 are separated by 107 nucleotides Shewana3_0444 and Shewana3_0445 are separated by 346 nucleotides Shewana3_0445 and Shewana3_0446 are separated by 3 nucleotides Shewana3_0446 and Shewana3_0447 are separated by 6 nucleotides
Shewana3_0443: Shewana3_0443 - permease (RefSeq), at 518,198 to 519,691
_0443
Shewana3_0444: Shewana3_0444 - band 7 protein (RefSeq), at 519,799 to 520,686
_0444
Shewana3_0445: Shewana3_0445 - hypothetical protein (RefSeq), at 521,033 to 521,287
_0445
Shewana3_0446: Shewana3_0446 - hypothetical protein (RefSeq), at 521,291 to 521,581
_0446
Shewana3_0447: Shewana3_0447 - response regulator receiver protein (RefSeq), at 521,588 to 523,207
_0447
Position (kb) 519
520
521 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 518.862 kb on + strand, within Shewana3_0443 at 518.862 kb on + strand, within Shewana3_0443 at 518.911 kb on + strand, within Shewana3_0443 at 518.934 kb on - strand, within Shewana3_0443 at 518.954 kb on + strand, within Shewana3_0443 at 519.038 kb on - strand, within Shewana3_0443 at 519.088 kb on + strand, within Shewana3_0443 at 519.103 kb on + strand, within Shewana3_0443 at 519.233 kb on - strand, within Shewana3_0443 at 519.241 kb on + strand, within Shewana3_0443 at 519.251 kb on + strand, within Shewana3_0443 at 519.295 kb on + strand, within Shewana3_0443 at 519.305 kb on + strand, within Shewana3_0443 at 519.372 kb on + strand, within Shewana3_0443 at 519.420 kb on + strand, within Shewana3_0443 at 519.445 kb on + strand, within Shewana3_0443 at 519.457 kb on + strand, within Shewana3_0443 at 519.479 kb on + strand, within Shewana3_0443 at 519.479 kb on + strand, within Shewana3_0443 at 519.487 kb on - strand, within Shewana3_0443 at 519.679 kb on + strand at 519.745 kb on + strand at 519.859 kb on - strand at 519.980 kb on + strand, within Shewana3_0444 at 519.980 kb on + strand, within Shewana3_0444 at 520.111 kb on + strand, within Shewana3_0444 at 520.441 kb on + strand, within Shewana3_0444 at 521.000 kb on + strand at 521.159 kb on - strand, within Shewana3_0445 at 521.193 kb on + strand, within Shewana3_0445 at 521.253 kb on - strand, within Shewana3_0445 at 521.343 kb on - strand, within Shewana3_0446 at 521.369 kb on + strand, within Shewana3_0446 at 521.369 kb on + strand, within Shewana3_0446 at 521.375 kb on + strand, within Shewana3_0446 at 521.375 kb on + strand, within Shewana3_0446 at 521.377 kb on - strand, within Shewana3_0446 at 521.438 kb on - strand, within Shewana3_0446 at 521.571 kb on - strand at 521.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 518,862 + Shewana3_0443 0.44 -1.0 518,862 + Shewana3_0443 0.44 +0.7 518,911 + Shewana3_0443 0.48 -0.9 518,934 - Shewana3_0443 0.49 +1.7 518,954 + Shewana3_0443 0.51 +0.3 519,038 - Shewana3_0443 0.56 +1.7 519,088 + Shewana3_0443 0.60 -0.3 519,103 + Shewana3_0443 0.61 -0.1 519,233 - Shewana3_0443 0.69 +1.7 519,241 + Shewana3_0443 0.70 -0.3 519,251 + Shewana3_0443 0.70 -1.4 519,295 + Shewana3_0443 0.73 -0.6 519,305 + Shewana3_0443 0.74 +0.2 519,372 + Shewana3_0443 0.79 -0.6 519,420 + Shewana3_0443 0.82 -1.7 519,445 + Shewana3_0443 0.83 +0.3 519,457 + Shewana3_0443 0.84 +0.3 519,479 + Shewana3_0443 0.86 -0.1 519,479 + Shewana3_0443 0.86 -0.4 519,487 - Shewana3_0443 0.86 +0.7 519,679 + -0.5 519,745 + -1.3 519,859 - +0.1 519,980 + Shewana3_0444 0.20 -0.2 519,980 + Shewana3_0444 0.20 +0.3 520,111 + Shewana3_0444 0.35 +0.4 520,441 + Shewana3_0444 0.72 -0.1 521,000 + +0.1 521,159 - Shewana3_0445 0.49 +1.7 521,193 + Shewana3_0445 0.63 -0.5 521,253 - Shewana3_0445 0.86 +2.2 521,343 - Shewana3_0446 0.18 -0.9 521,369 + Shewana3_0446 0.27 -3.1 521,369 + Shewana3_0446 0.27 +0.5 521,375 + Shewana3_0446 0.29 +0.6 521,375 + Shewana3_0446 0.29 +0.5 521,377 - Shewana3_0446 0.30 -0.5 521,438 - Shewana3_0446 0.51 -0.9 521,571 - +0.7 521,627 + -0.5
Or see this region's nucleotide sequence