Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides Shewana3_0376 and Shewana3_0377 are separated by 8 nucleotides Shewana3_0377 and Shewana3_0378 are separated by 18 nucleotides
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Shewana3_0377: Shewana3_0377 - hypothetical protein (RefSeq), at 418,996 to 419,703
_0377
Shewana3_0378: Shewana3_0378 - OmpA/MotB domain-containing protein (RefSeq), at 419,722 to 420,330
_0378
Position (kb) 417
418
419 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 416.148 kb on - strand at 417.114 kb on + strand at 417.461 kb on + strand, within Shewana3_0376 at 417.463 kb on + strand, within Shewana3_0376 at 417.505 kb on - strand, within Shewana3_0376 at 417.597 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.618 kb on + strand, within Shewana3_0376 at 417.830 kb on - strand, within Shewana3_0376 at 417.974 kb on + strand, within Shewana3_0376 at 418.143 kb on + strand, within Shewana3_0376 at 418.213 kb on + strand, within Shewana3_0376 at 418.226 kb on - strand, within Shewana3_0376 at 418.244 kb on - strand, within Shewana3_0376 at 418.256 kb on + strand, within Shewana3_0376 at 418.263 kb on - strand, within Shewana3_0376 at 418.344 kb on - strand, within Shewana3_0376 at 418.456 kb on - strand, within Shewana3_0376 at 418.472 kb on + strand, within Shewana3_0376 at 418.644 kb on + strand, within Shewana3_0376 at 418.644 kb on + strand, within Shewana3_0376 at 418.711 kb on - strand, within Shewana3_0376 at 418.859 kb on + strand at 418.897 kb on + strand at 418.979 kb on - strand at 418.996 kb on - strand at 419.047 kb on - strand at 419.077 kb on + strand, within Shewana3_0377 at 419.190 kb on + strand, within Shewana3_0377 at 419.195 kb on + strand, within Shewana3_0377 at 419.199 kb on - strand, within Shewana3_0377 at 419.219 kb on + strand, within Shewana3_0377 at 419.273 kb on + strand, within Shewana3_0377 at 419.318 kb on - strand, within Shewana3_0377 at 419.558 kb on - strand, within Shewana3_0377 at 419.560 kb on - strand, within Shewana3_0377 at 419.685 kb on + strand at 419.763 kb on + strand at 419.888 kb on - strand, within Shewana3_0378
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 416,148 - -1.4 417,114 + -0.8 417,461 + Shewana3_0376 0.20 +0.5 417,463 + Shewana3_0376 0.21 -0.1 417,505 - Shewana3_0376 0.23 +0.1 417,597 + Shewana3_0376 0.28 -1.7 417,618 + Shewana3_0376 0.29 -2.8 417,618 + Shewana3_0376 0.29 -2.6 417,618 + Shewana3_0376 0.29 -0.4 417,830 - Shewana3_0376 0.40 -1.3 417,974 + Shewana3_0376 0.47 -0.5 418,143 + Shewana3_0376 0.56 +0.3 418,213 + Shewana3_0376 0.60 -0.3 418,226 - Shewana3_0376 0.60 -1.0 418,244 - Shewana3_0376 0.61 -1.6 418,256 + Shewana3_0376 0.62 +0.1 418,263 - Shewana3_0376 0.62 -1.1 418,344 - Shewana3_0376 0.66 +0.7 418,456 - Shewana3_0376 0.72 -1.0 418,472 + Shewana3_0376 0.73 -1.1 418,644 + Shewana3_0376 0.82 -0.3 418,644 + Shewana3_0376 0.82 -1.1 418,711 - Shewana3_0376 0.86 +0.7 418,859 + +1.4 418,897 + +2.1 418,979 - -1.0 418,996 - +1.7 419,047 - -0.4 419,077 + Shewana3_0377 0.11 -1.1 419,190 + Shewana3_0377 0.27 +0.2 419,195 + Shewana3_0377 0.28 -0.4 419,199 - Shewana3_0377 0.29 +0.9 419,219 + Shewana3_0377 0.31 -1.2 419,273 + Shewana3_0377 0.39 +0.1 419,318 - Shewana3_0377 0.45 -0.4 419,558 - Shewana3_0377 0.79 +0.1 419,560 - Shewana3_0377 0.80 -0.3 419,685 + +0.7 419,763 + -0.5 419,888 - Shewana3_0378 0.27 +0.2
Or see this region's nucleotide sequence