Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0336 and Shewana3_0337 are separated by 8 nucleotides Shewana3_0337 and Shewana3_0338 overlap by 8 nucleotides Shewana3_0338 and Shewana3_0339 overlap by 4 nucleotides Shewana3_0339 and Shewana3_0340 are separated by 97 nucleotides Shewana3_0340 and Shewana3_0341 are separated by 29 nucleotides
Shewana3_0336: Shewana3_0336 - glycosyl transferase family protein (RefSeq), at 371,833 to 373,653
_0336
Shewana3_0337: Shewana3_0337 - thioester dehydrase family protein (RefSeq), at 373,662 to 374,036
_0337
Shewana3_0338: Shewana3_0338 - aconitate hydratase (RefSeq), at 374,029 to 375,393
_0338
Shewana3_0339: Shewana3_0339 - hypothetical protein (RefSeq), at 375,390 to 375,524
_0339
Shewana3_0340: Shewana3_0340 - transposase (RefSeq), at 375,622 to 376,017
_0340
Shewana3_0341: Shewana3_0341 - integrase catalytic subunit (RefSeq), at 376,047 to 376,850
_0341
Position (kb) 374
375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 373.128 kb on - strand, within Shewana3_0336 at 373.142 kb on - strand, within Shewana3_0336 at 373.142 kb on - strand, within Shewana3_0336 at 373.245 kb on + strand, within Shewana3_0336 at 373.264 kb on - strand, within Shewana3_0336 at 373.422 kb on - strand, within Shewana3_0336 at 373.455 kb on - strand, within Shewana3_0336 at 373.470 kb on + strand, within Shewana3_0336 at 373.546 kb on + strand at 373.549 kb on - strand at 373.588 kb on - strand at 374.140 kb on - strand at 374.254 kb on - strand, within Shewana3_0338 at 374.302 kb on - strand, within Shewana3_0338 at 374.507 kb on - strand, within Shewana3_0338 at 374.508 kb on - strand, within Shewana3_0338 at 374.594 kb on + strand, within Shewana3_0338 at 374.602 kb on - strand, within Shewana3_0338 at 374.604 kb on + strand, within Shewana3_0338 at 374.612 kb on - strand, within Shewana3_0338 at 374.612 kb on - strand, within Shewana3_0338 at 374.660 kb on - strand, within Shewana3_0338 at 374.701 kb on - strand, within Shewana3_0338 at 374.715 kb on + strand, within Shewana3_0338 at 374.739 kb on + strand, within Shewana3_0338 at 374.751 kb on + strand, within Shewana3_0338 at 374.763 kb on + strand, within Shewana3_0338 at 374.763 kb on + strand, within Shewana3_0338 at 374.943 kb on + strand, within Shewana3_0338 at 374.943 kb on + strand, within Shewana3_0338 at 374.943 kb on + strand, within Shewana3_0338 at 374.951 kb on - strand, within Shewana3_0338 at 374.951 kb on - strand, within Shewana3_0338 at 374.951 kb on - strand, within Shewana3_0338 at 374.951 kb on - strand, within Shewana3_0338 at 375.045 kb on + strand, within Shewana3_0338 at 375.066 kb on + strand, within Shewana3_0338 at 375.074 kb on - strand, within Shewana3_0338 at 375.179 kb on + strand, within Shewana3_0338 at 375.327 kb on + strand at 375.345 kb on - strand at 375.357 kb on + strand at 375.357 kb on + strand at 375.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 373,128 - Shewana3_0336 0.71 -0.8 373,142 - Shewana3_0336 0.72 -0.4 373,142 - Shewana3_0336 0.72 +0.0 373,245 + Shewana3_0336 0.78 +0.2 373,264 - Shewana3_0336 0.79 -0.4 373,422 - Shewana3_0336 0.87 -0.3 373,455 - Shewana3_0336 0.89 -1.6 373,470 + Shewana3_0336 0.90 -0.0 373,546 + +0.2 373,549 - -0.7 373,588 - +0.1 374,140 - +0.7 374,254 - Shewana3_0338 0.16 -0.7 374,302 - Shewana3_0338 0.20 -1.7 374,507 - Shewana3_0338 0.35 +0.0 374,508 - Shewana3_0338 0.35 -0.2 374,594 + Shewana3_0338 0.41 -0.7 374,602 - Shewana3_0338 0.42 +0.3 374,604 + Shewana3_0338 0.42 -0.8 374,612 - Shewana3_0338 0.43 -1.4 374,612 - Shewana3_0338 0.43 -0.0 374,660 - Shewana3_0338 0.46 -2.6 374,701 - Shewana3_0338 0.49 -0.1 374,715 + Shewana3_0338 0.50 -1.0 374,739 + Shewana3_0338 0.52 -0.7 374,751 + Shewana3_0338 0.53 +0.4 374,763 + Shewana3_0338 0.54 -0.5 374,763 + Shewana3_0338 0.54 -0.1 374,943 + Shewana3_0338 0.67 +0.5 374,943 + Shewana3_0338 0.67 +0.5 374,943 + Shewana3_0338 0.67 -0.1 374,951 - Shewana3_0338 0.68 +0.4 374,951 - Shewana3_0338 0.68 -0.1 374,951 - Shewana3_0338 0.68 -2.9 374,951 - Shewana3_0338 0.68 -3.1 375,045 + Shewana3_0338 0.74 -0.3 375,066 + Shewana3_0338 0.76 +3.3 375,074 - Shewana3_0338 0.77 +0.5 375,179 + Shewana3_0338 0.84 +0.6 375,327 + +0.5 375,345 - +0.8 375,357 + +0.4 375,357 + +1.4 375,518 - -0.2
Or see this region's nucleotide sequence