Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0269 and Shewana3_0270 are separated by 117 nucleotides Shewana3_0270 and Shewana3_0271 are separated by 36 nucleotides Shewana3_0271 and Shewana3_0272 are separated by 81 nucleotides
Shewana3_0269: Shewana3_0269 - diguanylate cyclase (RefSeq), at 302,810 to 304,267
_0269
Shewana3_0270: Shewana3_0270 - MOSC domain-containing protein (RefSeq), at 304,385 to 305,080
_0270
Shewana3_0271: Shewana3_0271 - hypothetical protein (RefSeq), at 305,117 to 305,473
_0271
Shewana3_0272: Shewana3_0272 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 305,555 to 307,471
_0272
Position (kb) 305
306 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 304.149 kb on + strand at 304.149 kb on + strand at 304.157 kb on - strand at 304.272 kb on - strand at 304.352 kb on + strand at 304.536 kb on + strand, within Shewana3_0270 at 304.603 kb on - strand, within Shewana3_0270 at 304.795 kb on - strand, within Shewana3_0270 at 304.804 kb on + strand, within Shewana3_0270 at 304.880 kb on - strand, within Shewana3_0270 at 304.880 kb on - strand, within Shewana3_0270 at 305.104 kb on - strand at 305.437 kb on - strand, within Shewana3_0271 at 305.554 kb on - strand at 305.608 kb on + strand at 305.688 kb on - strand at 305.758 kb on + strand, within Shewana3_0272 at 305.758 kb on + strand, within Shewana3_0272 at 305.837 kb on + strand, within Shewana3_0272 at 305.845 kb on - strand, within Shewana3_0272 at 305.845 kb on - strand, within Shewana3_0272 at 305.845 kb on - strand, within Shewana3_0272 at 305.929 kb on + strand, within Shewana3_0272 at 305.974 kb on - strand, within Shewana3_0272 at 306.085 kb on - strand, within Shewana3_0272 at 306.085 kb on - strand, within Shewana3_0272 at 306.085 kb on - strand, within Shewana3_0272 at 306.255 kb on - strand, within Shewana3_0272 at 306.377 kb on - strand, within Shewana3_0272
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 304,149 + +0.7 304,149 + -0.4 304,157 - -0.8 304,272 - -0.4 304,352 + -0.1 304,536 + Shewana3_0270 0.22 +0.4 304,603 - Shewana3_0270 0.31 -0.3 304,795 - Shewana3_0270 0.59 -0.6 304,804 + Shewana3_0270 0.60 -0.2 304,880 - Shewana3_0270 0.71 -0.3 304,880 - Shewana3_0270 0.71 +0.5 305,104 - -0.1 305,437 - Shewana3_0271 0.90 -1.1 305,554 - -1.1 305,608 + -2.4 305,688 - -1.6 305,758 + Shewana3_0272 0.11 -1.3 305,758 + Shewana3_0272 0.11 -3.0 305,837 + Shewana3_0272 0.15 -3.3 305,845 - Shewana3_0272 0.15 -2.4 305,845 - Shewana3_0272 0.15 -1.8 305,845 - Shewana3_0272 0.15 -1.5 305,929 + Shewana3_0272 0.20 -1.8 305,974 - Shewana3_0272 0.22 -2.7 306,085 - Shewana3_0272 0.28 -1.1 306,085 - Shewana3_0272 0.28 -2.0 306,085 - Shewana3_0272 0.28 -3.0 306,255 - Shewana3_0272 0.37 -2.6 306,377 - Shewana3_0272 0.43 -2.0
Or see this region's nucleotide sequence