Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0233 and Shewana3_0234 are separated by 399 nucleotides Shewana3_0234 and Shewana3_0235 are separated by 11 nucleotides Shewana3_0235 and Shewana3_0236 overlap by 4 nucleotides
Shewana3_0233: Shewana3_0233 - TPR repeat-containing protein (RefSeq), at 261,588 to 262,835
_0233
Shewana3_0234: Shewana3_0234 - cytochrome c-type biogenesis protein CcmF (RefSeq), at 263,235 to 265,214
_0234
Shewana3_0235: Shewana3_0235 - periplasmic protein thiol--disulphide oxidoreductase DsbE (RefSeq), at 265,226 to 265,780
_0235
Shewana3_0236: Shewana3_0236 - cytochrome C biogenesis protein (RefSeq), at 265,777 to 266,256
_0236
Position (kb) 263
264
265
266 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 262.247 kb on - strand, within Shewana3_0233 at 262.317 kb on + strand, within Shewana3_0233 at 262.402 kb on + strand, within Shewana3_0233 at 262.591 kb on - strand, within Shewana3_0233 at 262.655 kb on + strand, within Shewana3_0233 at 262.702 kb on + strand, within Shewana3_0233 at 262.741 kb on - strand at 262.743 kb on - strand at 262.769 kb on + strand at 262.792 kb on - strand at 262.792 kb on - strand at 262.797 kb on - strand at 262.936 kb on + strand at 262.944 kb on - strand at 262.944 kb on - strand at 263.264 kb on + strand at 263.264 kb on + strand at 263.272 kb on - strand at 263.414 kb on - strand at 263.421 kb on + strand at 263.443 kb on + strand, within Shewana3_0234 at 263.495 kb on + strand, within Shewana3_0234 at 263.514 kb on - strand, within Shewana3_0234 at 263.528 kb on - strand, within Shewana3_0234 at 263.533 kb on - strand, within Shewana3_0234 at 263.724 kb on - strand, within Shewana3_0234 at 263.757 kb on + strand, within Shewana3_0234 at 263.816 kb on + strand, within Shewana3_0234 at 263.828 kb on + strand, within Shewana3_0234 at 263.851 kb on + strand, within Shewana3_0234 at 264.003 kb on + strand, within Shewana3_0234 at 264.130 kb on + strand, within Shewana3_0234 at 264.247 kb on + strand, within Shewana3_0234 at 264.774 kb on + strand, within Shewana3_0234 at 264.829 kb on + strand, within Shewana3_0234 at 264.829 kb on + strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 265.112 kb on + strand at 265.395 kb on - strand, within Shewana3_0235 at 265.532 kb on - strand, within Shewana3_0235 at 265.562 kb on + strand, within Shewana3_0235 at 265.613 kb on + strand, within Shewana3_0235 at 265.765 kb on + strand at 265.842 kb on + strand, within Shewana3_0236 at 265.851 kb on + strand, within Shewana3_0236 at 265.915 kb on - strand, within Shewana3_0236 at 265.952 kb on + strand, within Shewana3_0236 at 266.018 kb on - strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 262,247 - Shewana3_0233 0.53 +0.6 262,317 + Shewana3_0233 0.58 +0.9 262,402 + Shewana3_0233 0.65 -0.9 262,591 - Shewana3_0233 0.80 +2.5 262,655 + Shewana3_0233 0.85 +3.5 262,702 + Shewana3_0233 0.89 +1.1 262,741 - +3.0 262,743 - +3.3 262,769 + +1.2 262,792 - +3.4 262,792 - +2.5 262,797 - +1.7 262,936 + +0.7 262,944 - +1.3 262,944 - -0.3 263,264 + +2.9 263,264 + +3.0 263,272 - -0.3 263,414 - -0.3 263,421 + +2.3 263,443 + Shewana3_0234 0.11 +2.6 263,495 + Shewana3_0234 0.13 +1.4 263,514 - Shewana3_0234 0.14 +3.9 263,528 - Shewana3_0234 0.15 +4.2 263,533 - Shewana3_0234 0.15 +2.7 263,724 - Shewana3_0234 0.25 +3.2 263,757 + Shewana3_0234 0.26 +3.4 263,816 + Shewana3_0234 0.29 +2.1 263,828 + Shewana3_0234 0.30 +3.2 263,851 + Shewana3_0234 0.31 +3.2 264,003 + Shewana3_0234 0.39 +3.2 264,130 + Shewana3_0234 0.45 +3.7 264,247 + Shewana3_0234 0.51 +3.3 264,774 + Shewana3_0234 0.78 +3.6 264,829 + Shewana3_0234 0.81 +1.0 264,829 + Shewana3_0234 0.81 +2.0 264,874 - Shewana3_0234 0.83 +3.4 264,874 - Shewana3_0234 0.83 +1.5 265,112 + -0.6 265,395 - Shewana3_0235 0.30 +3.8 265,532 - Shewana3_0235 0.55 +1.7 265,562 + Shewana3_0235 0.61 +3.6 265,613 + Shewana3_0235 0.70 +2.0 265,765 + -0.2 265,842 + Shewana3_0236 0.14 +2.9 265,851 + Shewana3_0236 0.15 +4.0 265,915 - Shewana3_0236 0.29 +4.9 265,952 + Shewana3_0236 0.36 +3.5 266,018 - Shewana3_0236 0.50 +0.1 266,113 + Shewana3_0236 0.70 +3.2 266,113 + Shewana3_0236 0.70 +2.5
Or see this region's nucleotide sequence