Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0131 and Shewana3_0132 are separated by 247 nucleotides Shewana3_0132 and Shewana3_0133 are separated by 75 nucleotides
Shewana3_0131: Shewana3_0131 - molybdopterin molybdochelatase (RefSeq), at 146,271 to 147,605
_0131
Shewana3_0132: Shewana3_0132 - Ferritin, Dps family protein (RefSeq), at 147,853 to 148,389
_0132
Shewana3_0133: Shewana3_0133 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (RefSeq), at 148,465 to 151,698
_0133
Position (kb) 147
148
149 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 146.990 kb on - strand, within Shewana3_0131 at 147.011 kb on - strand, within Shewana3_0131 at 147.106 kb on + strand, within Shewana3_0131 at 147.106 kb on + strand, within Shewana3_0131 at 147.106 kb on + strand, within Shewana3_0131 at 147.106 kb on + strand, within Shewana3_0131 at 147.106 kb on + strand, within Shewana3_0131 at 147.108 kb on - strand, within Shewana3_0131 at 147.108 kb on - strand, within Shewana3_0131 at 147.108 kb on - strand, within Shewana3_0131 at 147.108 kb on - strand, within Shewana3_0131 at 147.114 kb on - strand, within Shewana3_0131 at 147.114 kb on - strand, within Shewana3_0131 at 147.114 kb on - strand, within Shewana3_0131 at 147.114 kb on - strand, within Shewana3_0131 at 147.140 kb on + strand, within Shewana3_0131 at 147.439 kb on + strand, within Shewana3_0131 at 147.439 kb on + strand, within Shewana3_0131 at 147.439 kb on + strand, within Shewana3_0131 at 147.439 kb on + strand, within Shewana3_0131 at 147.528 kb on - strand at 147.581 kb on + strand at 147.592 kb on + strand at 147.602 kb on + strand at 147.661 kb on - strand at 147.949 kb on + strand, within Shewana3_0132 at 148.042 kb on + strand, within Shewana3_0132 at 148.218 kb on + strand, within Shewana3_0132 at 148.646 kb on + strand at 148.690 kb on - strand at 148.716 kb on - strand at 148.804 kb on - strand, within Shewana3_0133 at 148.861 kb on + strand, within Shewana3_0133 at 148.931 kb on + strand, within Shewana3_0133 at 148.945 kb on + strand, within Shewana3_0133 at 148.945 kb on - strand, within Shewana3_0133 at 148.975 kb on + strand, within Shewana3_0133 at 149.027 kb on + strand, within Shewana3_0133 at 149.104 kb on + strand, within Shewana3_0133 at 149.132 kb on + strand, within Shewana3_0133 at 149.145 kb on - strand, within Shewana3_0133 at 149.150 kb on - strand, within Shewana3_0133 at 149.150 kb on - strand, within Shewana3_0133 at 149.150 kb on - strand, within Shewana3_0133 at 149.234 kb on + strand, within Shewana3_0133 at 149.277 kb on - strand, within Shewana3_0133
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 146,990 - Shewana3_0131 0.54 +1.1 147,011 - Shewana3_0131 0.55 -0.1 147,106 + Shewana3_0131 0.63 +1.0 147,106 + Shewana3_0131 0.63 +1.6 147,106 + Shewana3_0131 0.63 +0.3 147,106 + Shewana3_0131 0.63 +1.0 147,106 + Shewana3_0131 0.63 +1.7 147,108 - Shewana3_0131 0.63 +0.9 147,108 - Shewana3_0131 0.63 -1.3 147,108 - Shewana3_0131 0.63 +1.1 147,108 - Shewana3_0131 0.63 +0.4 147,114 - Shewana3_0131 0.63 +0.2 147,114 - Shewana3_0131 0.63 -0.4 147,114 - Shewana3_0131 0.63 +0.9 147,114 - Shewana3_0131 0.63 +0.8 147,140 + Shewana3_0131 0.65 -0.7 147,439 + Shewana3_0131 0.87 +0.4 147,439 + Shewana3_0131 0.87 -0.2 147,439 + Shewana3_0131 0.87 +0.3 147,439 + Shewana3_0131 0.87 +0.8 147,528 - -0.1 147,581 + -2.9 147,592 + -1.2 147,602 + -0.9 147,661 - +0.3 147,949 + Shewana3_0132 0.18 -1.1 148,042 + Shewana3_0132 0.35 -2.0 148,218 + Shewana3_0132 0.68 -1.9 148,646 + -1.1 148,690 - -0.9 148,716 - -0.7 148,804 - Shewana3_0133 0.10 -0.6 148,861 + Shewana3_0133 0.12 -0.4 148,931 + Shewana3_0133 0.14 -0.5 148,945 + Shewana3_0133 0.15 -0.2 148,945 - Shewana3_0133 0.15 -1.7 148,975 + Shewana3_0133 0.16 -3.9 149,027 + Shewana3_0133 0.17 -2.2 149,104 + Shewana3_0133 0.20 -0.8 149,132 + Shewana3_0133 0.21 -0.9 149,145 - Shewana3_0133 0.21 -0.8 149,150 - Shewana3_0133 0.21 -1.9 149,150 - Shewana3_0133 0.21 -1.2 149,150 - Shewana3_0133 0.21 -0.4 149,234 + Shewana3_0133 0.24 -0.6 149,277 - Shewana3_0133 0.25 -3.9
Or see this region's nucleotide sequence