Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4175 and Shewana3_4176 are separated by 414 nucleotides Shewana3_4176 and Shewana3_4177 overlap by 26 nucleotides
Shewana3_4175: Shewana3_4175 - hypothetical protein (RefSeq), at 41,152 to 42,258
_4175
Shewana3_4176: Shewana3_4176 - hypothetical protein (RefSeq), at 42,673 to 43,521
_4176
Shewana3_4177: Shewana3_4177 - CBS domain-containing protein (RefSeq), at 43,496 to 44,170
_4177
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1 at 41.863 kb on + strand, within Shewana3_4175 at 41.917 kb on + strand, within Shewana3_4175 at 42.066 kb on + strand, within Shewana3_4175 at 42.122 kb on + strand, within Shewana3_4175 at 42.125 kb on - strand, within Shewana3_4175 at 42.383 kb on + strand at 42.391 kb on - strand at 42.391 kb on - strand at 42.435 kb on + strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.443 kb on - strand at 42.629 kb on + strand at 42.630 kb on + strand at 42.630 kb on + strand at 42.650 kb on - strand at 42.765 kb on - strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176 at 43.253 kb on + strand, within Shewana3_4176 at 43.284 kb on + strand, within Shewana3_4176 at 43.561 kb on - strand at 43.566 kb on - strand, within Shewana3_4177 at 43.821 kb on - strand, within Shewana3_4177 at 43.827 kb on - strand, within Shewana3_4177 at 43.849 kb on + strand, within Shewana3_4177 at 44.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 41,863 + Shewana3_4175 0.64 +0.2 41,917 + Shewana3_4175 0.69 -2.0 42,066 + Shewana3_4175 0.83 -0.3 42,122 + Shewana3_4175 0.88 -0.4 42,125 - Shewana3_4175 0.88 +1.2 42,383 + -0.8 42,391 - +1.2 42,391 - +1.2 42,435 + -0.8 42,443 - -2.0 42,443 - -0.8 42,443 - -0.8 42,629 + +1.2 42,630 + +1.2 42,630 + +0.5 42,650 - -1.1 42,765 - Shewana3_4176 0.11 -0.4 43,253 + Shewana3_4176 0.68 +1.7 43,253 + Shewana3_4176 0.68 +1.4 43,284 + Shewana3_4176 0.72 -1.6 43,561 - -1.4 43,566 - Shewana3_4177 0.10 -0.7 43,821 - Shewana3_4177 0.48 -1.2 43,827 - Shewana3_4177 0.49 -0.8 43,849 + Shewana3_4177 0.52 -1.1 44,367 - +1.2
Or see this region's nucleotide sequence