Experiment: Rifampicin 0.0003125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiL and Synpcc7942_2568 are separated by 11 nucleotides Synpcc7942_2568 and pyrF overlap by 4 nucleotides pyrF and tyrS are separated by 3 nucleotides
Synpcc7942_2567: thiL - thiamine monophosphate kinase, at 2,646,633 to 2,647,616
thiL
Synpcc7942_2568: Synpcc7942_2568 - hypothetical protein, at 2,647,628 to 2,648,101
_2568
Synpcc7942_2569: pyrF - orotidine 5'-phosphate decarboxylase, at 2,648,098 to 2,648,820
pyrF
Synpcc7942_2570: tyrS - tyrosyl-tRNA synthetase, at 2,648,824 to 2,650,032
tyrS
Position (kb)
2647
2648
2649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2647.618 kb on - strand at 2647.626 kb on + strand at 2647.626 kb on + strand at 2647.672 kb on + strand at 2647.712 kb on - strand, within Synpcc7942_2568 at 2647.725 kb on + strand, within Synpcc7942_2568 at 2647.759 kb on + strand, within Synpcc7942_2568 at 2647.795 kb on + strand, within Synpcc7942_2568 at 2647.807 kb on + strand, within Synpcc7942_2568 at 2647.807 kb on + strand, within Synpcc7942_2568 at 2647.861 kb on + strand, within Synpcc7942_2568 at 2647.931 kb on - strand, within Synpcc7942_2568 at 2647.957 kb on + strand, within Synpcc7942_2568 at 2647.958 kb on + strand, within Synpcc7942_2568 at 2647.980 kb on - strand, within Synpcc7942_2568 at 2648.038 kb on - strand, within Synpcc7942_2568 at 2648.043 kb on + strand, within Synpcc7942_2568 at 2648.051 kb on - strand, within Synpcc7942_2568 at 2648.075 kb on + strand at 2648.097 kb on + strand at 2648.107 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.0003125 mg/ml remove 2,647,618 - +0.4 2,647,626 + +0.4 2,647,626 + +0.5 2,647,672 + -0.4 2,647,712 - Synpcc7942_2568 0.18 -1.4 2,647,725 + Synpcc7942_2568 0.20 -1.3 2,647,759 + Synpcc7942_2568 0.28 -1.4 2,647,795 + Synpcc7942_2568 0.35 -0.2 2,647,807 + Synpcc7942_2568 0.38 -2.1 2,647,807 + Synpcc7942_2568 0.38 -1.4 2,647,861 + Synpcc7942_2568 0.49 -1.2 2,647,931 - Synpcc7942_2568 0.64 -1.1 2,647,957 + Synpcc7942_2568 0.69 -0.6 2,647,958 + Synpcc7942_2568 0.70 -2.7 2,647,980 - Synpcc7942_2568 0.74 -0.8 2,648,038 - Synpcc7942_2568 0.86 -1.2 2,648,043 + Synpcc7942_2568 0.88 -1.3 2,648,051 - Synpcc7942_2568 0.89 -2.8 2,648,075 + -0.4 2,648,097 + +0.2 2,648,107 - +2.2
Or see this region's nucleotide sequence