Experiment: Recovery broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17145 and BBR_RS20995 overlap by 36 nucleotides BBR_RS20995 and BBR_RS17150 overlap by 4 nucleotides BBR_RS17150 and BBR_RS17155 are separated by 111 nucleotides BBR_RS17155 and BBR_RS17160 are separated by 12 nucleotides
BBR_RS17145: BBR_RS17145 - DivIVA domain-containing protein, at 1,625,554 to 1,626,903
_RS17145
BBR_RS20995: BBR_RS20995 - hypothetical protein, at 1,626,868 to 1,627,047
_RS20995
BBR_RS17150: BBR_RS17150 - YggT family protein, at 1,627,044 to 1,627,346
_RS17150
BBR_RS17155: BBR_RS17155 - cell division protein SepF, at 1,627,458 to 1,627,934
_RS17155
BBR_RS17160: BBR_RS17160 - cell division protein FtsZ, at 1,627,947 to 1,629,149
_RS17160
Position (kb)
1627
1628 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1627.066 kb on - strand at 1627.066 kb on - strand at 1627.089 kb on + strand, within BBR_RS17150 at 1627.095 kb on - strand, within BBR_RS17150 at 1627.095 kb on - strand, within BBR_RS17150 at 1627.123 kb on - strand, within BBR_RS17150 at 1627.192 kb on + strand, within BBR_RS17150 at 1627.240 kb on + strand, within BBR_RS17150 at 1627.254 kb on + strand, within BBR_RS17150 at 1627.310 kb on + strand, within BBR_RS17150 at 1627.348 kb on + strand at 1627.444 kb on + strand at 1627.531 kb on - strand, within BBR_RS17155
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth remove 1,627,066 - -0.6 1,627,066 - -0.6 1,627,089 + BBR_RS17150 0.15 +1.2 1,627,095 - BBR_RS17150 0.17 -0.2 1,627,095 - BBR_RS17150 0.17 +0.1 1,627,123 - BBR_RS17150 0.26 +0.2 1,627,192 + BBR_RS17150 0.49 -0.8 1,627,240 + BBR_RS17150 0.65 -1.1 1,627,254 + BBR_RS17150 0.69 -1.6 1,627,310 + BBR_RS17150 0.88 -0.9 1,627,348 + -0.3 1,627,444 + -0.0 1,627,531 - BBR_RS17155 0.15 -3.1
Or see this region's nucleotide sequence