Strain Fitness in Shewanella sp. ANA-3 around Shewana3_2455

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntushA and Shewana3_2455 are separated by 364 nucleotidesShewana3_2455 and Shewana3_2456 are separated by 52 nucleotidesShewana3_2456 and Shewana3_2457 are separated by 344 nucleotides Shewana3_2454: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,941,773 to 2,943,494 ushA Shewana3_2455: Shewana3_2455 - phospholipase/carboxylesterase (RefSeq), at 2,943,859 to 2,944,524 _2455 Shewana3_2456: Shewana3_2456 - hypothetical protein (RefSeq), at 2,944,577 to 2,944,846 _2456 Shewana3_2457: Shewana3_2457 - peptidase M1, membrane alanine aminopeptidase (RefSeq), at 2,945,191 to 2,947,119 _2457 Position (kb) 2943 2944 2945Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2943.092 kb on - strand, within ushAat 2943.180 kb on + strand, within ushAat 2943.389 kb on - strandat 2943.437 kb on - strandat 2943.475 kb on + strandat 2944.018 kb on - strand, within Shewana3_2455at 2944.192 kb on + strand, within Shewana3_2455at 2944.254 kb on + strand, within Shewana3_2455at 2944.262 kb on - strand, within Shewana3_2455at 2944.262 kb on - strand, within Shewana3_2455at 2944.265 kb on + strand, within Shewana3_2455at 2944.281 kb on - strand, within Shewana3_2455at 2944.398 kb on - strand, within Shewana3_2455at 2944.698 kb on - strand, within Shewana3_2456at 2944.711 kb on - strand, within Shewana3_2456at 2944.776 kb on + strand, within Shewana3_2456at 2944.910 kb on + strandat 2944.910 kb on + strandat 2944.918 kb on - strandat 2944.918 kb on - strandat 2944.918 kb on - strandat 2944.995 kb on - strandat 2945.212 kb on - strandat 2945.235 kb on - strandat 2945.273 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,943,092 - ushA Shewana3_2454 0.77 -0.2
2,943,180 + ushA Shewana3_2454 0.82 +1.4
2,943,389 - +1.6
2,943,437 - +1.1
2,943,475 + +1.4
2,944,018 - Shewana3_2455 0.24 -4.1
2,944,192 + Shewana3_2455 0.50 +1.4
2,944,254 + Shewana3_2455 0.59 +1.4
2,944,262 - Shewana3_2455 0.61 -3.0
2,944,262 - Shewana3_2455 0.61 -2.8
2,944,265 + Shewana3_2455 0.61 +1.4
2,944,281 - Shewana3_2455 0.63 +0.6
2,944,398 - Shewana3_2455 0.81 -1.1
2,944,698 - Shewana3_2456 0.45 -0.4
2,944,711 - Shewana3_2456 0.50 -0.2
2,944,776 + Shewana3_2456 0.74 +1.4
2,944,910 + +1.4
2,944,910 + +1.4
2,944,918 - -0.6
2,944,918 - -0.8
2,944,918 - +0.7
2,944,995 - -0.0
2,945,212 - -0.0
2,945,235 - +1.4
2,945,273 - -1.2

Or see this region's nucleotide sequence