Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1432 and Shewana3_1433 are separated by 182 nucleotides Shewana3_1433 and queA are separated by 64 nucleotides queA and tgt are separated by 161 nucleotides
Shewana3_1432: Shewana3_1432 - hypothetical protein (RefSeq), at 1,662,021 to 1,662,647
_1432
Shewana3_1433: Shewana3_1433 - hypothetical protein (RefSeq), at 1,662,830 to 1,663,033
_1433
Shewana3_1434: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (RefSeq), at 1,663,098 to 1,664,135
queA
Shewana3_1435: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 1,664,297 to 1,665,421
tgt
Position (kb)
1663
1664
1665 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1662.280 kb on + strand, within Shewana3_1432 at 1662.292 kb on + strand, within Shewana3_1432 at 1662.293 kb on - strand, within Shewana3_1432 at 1662.300 kb on - strand, within Shewana3_1432 at 1662.420 kb on + strand, within Shewana3_1432 at 1662.464 kb on - strand, within Shewana3_1432 at 1662.493 kb on + strand, within Shewana3_1432 at 1662.501 kb on - strand, within Shewana3_1432 at 1662.530 kb on - strand, within Shewana3_1432 at 1662.770 kb on + strand at 1663.090 kb on - strand at 1663.092 kb on - strand at 1663.179 kb on - strand at 1663.419 kb on - strand, within queA at 1663.432 kb on + strand, within queA at 1663.724 kb on + strand, within queA at 1663.732 kb on - strand, within queA at 1663.797 kb on + strand, within queA at 1664.046 kb on + strand at 1664.234 kb on - strand at 1664.512 kb on + strand, within tgt at 1664.552 kb on + strand, within tgt at 1664.771 kb on + strand, within tgt at 1664.785 kb on + strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,662,280 + Shewana3_1432 0.41 -0.0 1,662,292 + Shewana3_1432 0.43 +1.2 1,662,293 - Shewana3_1432 0.43 +0.8 1,662,300 - Shewana3_1432 0.44 -0.4 1,662,420 + Shewana3_1432 0.64 +0.6 1,662,464 - Shewana3_1432 0.71 -0.4 1,662,493 + Shewana3_1432 0.75 +2.0 1,662,501 - Shewana3_1432 0.77 +1.0 1,662,530 - Shewana3_1432 0.81 +0.2 1,662,770 + -0.0 1,663,090 - +0.6 1,663,092 - +1.6 1,663,179 - +0.6 1,663,419 - queA Shewana3_1434 0.31 +1.6 1,663,432 + queA Shewana3_1434 0.32 -3.1 1,663,724 + queA Shewana3_1434 0.60 -1.0 1,663,732 - queA Shewana3_1434 0.61 -0.0 1,663,797 + queA Shewana3_1434 0.67 -1.3 1,664,046 + -1.0 1,664,234 - +1.6 1,664,512 + tgt Shewana3_1435 0.19 -2.4 1,664,552 + tgt Shewana3_1435 0.23 -1.1 1,664,771 + tgt Shewana3_1435 0.42 -2.2 1,664,785 + tgt Shewana3_1435 0.43 -0.8
Or see this region's nucleotide sequence