Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhF and Shewana3_1358 are separated by 4 nucleotides Shewana3_1358 and fliA overlap by 8 nucleotides fliA and Shewana3_1360 are separated by 47 nucleotides Shewana3_1360 and Shewana3_1361 are separated by 13 nucleotides
Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345
flhF
Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231
_1358
Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937
fliA
Shewana3_1360: Shewana3_1360 - response regulator receiver protein (RefSeq), at 1,572,985 to 1,573,368
_1360
Shewana3_1361: Shewana3_1361 - chemotaxis phosphatase, CheZ (RefSeq), at 1,573,382 to 1,574,119
_1361
Position (kb)
1572
1573 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1571.587 kb on + strand, within Shewana3_1358 at 1571.602 kb on + strand, within Shewana3_1358 at 1571.812 kb on + strand, within Shewana3_1358 at 1572.075 kb on + strand, within Shewana3_1358 at 1572.239 kb on + strand at 1572.288 kb on - strand at 1572.376 kb on + strand, within fliA at 1572.524 kb on + strand, within fliA at 1572.806 kb on + strand, within fliA at 1572.907 kb on + strand at 1573.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,571,587 + Shewana3_1358 0.27 -6.9 1,571,602 + Shewana3_1358 0.29 -6.8 1,571,812 + Shewana3_1358 0.52 -5.8 1,572,075 + Shewana3_1358 0.82 -3.0 1,572,239 + -6.8 1,572,288 - +1.6 1,572,376 + fliA Shewana3_1359 0.21 -5.0 1,572,524 + fliA Shewana3_1359 0.42 -3.8 1,572,806 + fliA Shewana3_1359 0.82 -3.0 1,572,907 + -4.0 1,573,403 - +0.0
Or see this region's nucleotide sequence