Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1359

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhF and Shewana3_1358 are separated by 4 nucleotidesShewana3_1358 and fliA overlap by 8 nucleotidesfliA and Shewana3_1360 are separated by 47 nucleotidesShewana3_1360 and Shewana3_1361 are separated by 13 nucleotides Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345 flhF Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231 _1358 Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937 fliA Shewana3_1360: Shewana3_1360 - response regulator receiver protein (RefSeq), at 1,572,985 to 1,573,368 _1360 Shewana3_1361: Shewana3_1361 - chemotaxis phosphatase, CheZ (RefSeq), at 1,573,382 to 1,574,119 _1361 Position (kb) 1572 1573Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1571.587 kb on + strand, within Shewana3_1358at 1571.602 kb on + strand, within Shewana3_1358at 1571.812 kb on + strand, within Shewana3_1358at 1572.075 kb on + strand, within Shewana3_1358at 1572.239 kb on + strandat 1572.288 kb on - strandat 1572.376 kb on + strand, within fliAat 1572.524 kb on + strand, within fliAat 1572.806 kb on + strand, within fliAat 1572.907 kb on + strandat 1573.403 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,571,587 + Shewana3_1358 0.27 -6.9
1,571,602 + Shewana3_1358 0.29 -6.8
1,571,812 + Shewana3_1358 0.52 -5.8
1,572,075 + Shewana3_1358 0.82 -3.0
1,572,239 + -6.8
1,572,288 - +1.6
1,572,376 + fliA Shewana3_1359 0.21 -5.0
1,572,524 + fliA Shewana3_1359 0.42 -3.8
1,572,806 + fliA Shewana3_1359 0.82 -3.0
1,572,907 + -4.0
1,573,403 - +0.0

Or see this region's nucleotide sequence