Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhA and flhF are separated by 11 nucleotides flhF and Shewana3_1358 are separated by 4 nucleotides Shewana3_1358 and fliA overlap by 8 nucleotides
Shewana3_1356: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 1,567,858 to 1,569,957
flhA
Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345
flhF
Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231
_1358
Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937
fliA
Position (kb)
1569
1570
1571
1572 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1569.001 kb on + strand, within flhA at 1569.099 kb on + strand, within flhA at 1569.213 kb on + strand, within flhA at 1569.221 kb on + strand, within flhA at 1569.269 kb on + strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.345 kb on - strand, within flhA at 1570.008 kb on + strand at 1570.180 kb on + strand, within flhF at 1570.239 kb on + strand, within flhF at 1570.329 kb on + strand, within flhF at 1570.332 kb on + strand, within flhF at 1570.349 kb on + strand, within flhF at 1570.690 kb on + strand, within flhF at 1570.767 kb on + strand, within flhF at 1570.878 kb on + strand, within flhF at 1571.047 kb on + strand, within flhF at 1571.052 kb on + strand, within flhF at 1571.110 kb on + strand, within flhF at 1571.587 kb on + strand, within Shewana3_1358 at 1571.602 kb on + strand, within Shewana3_1358 at 1571.812 kb on + strand, within Shewana3_1358 at 1572.075 kb on + strand, within Shewana3_1358 at 1572.239 kb on + strand at 1572.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,569,001 + flhA Shewana3_1356 0.54 -3.4 1,569,099 + flhA Shewana3_1356 0.59 -6.0 1,569,213 + flhA Shewana3_1356 0.65 -4.8 1,569,221 + flhA Shewana3_1356 0.65 -2.4 1,569,269 + flhA Shewana3_1356 0.67 -3.3 1,569,277 - flhA Shewana3_1356 0.68 +1.6 1,569,277 - flhA Shewana3_1356 0.68 +0.6 1,569,277 - flhA Shewana3_1356 0.68 +1.6 1,569,345 - flhA Shewana3_1356 0.71 -1.0 1,570,008 + -6.0 1,570,180 + flhF Shewana3_1357 0.15 -4.9 1,570,239 + flhF Shewana3_1357 0.20 -6.4 1,570,329 + flhF Shewana3_1357 0.26 -5.5 1,570,332 + flhF Shewana3_1357 0.26 -2.5 1,570,349 + flhF Shewana3_1357 0.28 -3.9 1,570,690 + flhF Shewana3_1357 0.52 -7.0 1,570,767 + flhF Shewana3_1357 0.58 -6.1 1,570,878 + flhF Shewana3_1357 0.66 -6.5 1,571,047 + flhF Shewana3_1357 0.78 -5.7 1,571,052 + flhF Shewana3_1357 0.79 -3.8 1,571,110 + flhF Shewana3_1357 0.83 -5.5 1,571,587 + Shewana3_1358 0.27 -6.9 1,571,602 + Shewana3_1358 0.29 -6.8 1,571,812 + Shewana3_1358 0.52 -5.8 1,572,075 + Shewana3_1358 0.82 -3.0 1,572,239 + -6.8 1,572,288 - +1.6
Or see this region's nucleotide sequence