Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1347 and fliL are separated by 82 nucleotides fliL and fliM are separated by 40 nucleotides fliM and fliN are separated by 12 nucleotides fliN and Shewana3_1351 are separated by 33 nucleotides Shewana3_1351 and fliP overlap by 4 nucleotides
Shewana3_1347: Shewana3_1347 - flagellar hook-length control protein (RefSeq), at 1,560,513 to 1,562,252
_1347
Shewana3_1348: fliL - flagellar basal body-associated protein FliL (RefSeq), at 1,562,335 to 1,562,859
fliL
Shewana3_1349: fliM - flagellar motor switch protein FliM (RefSeq), at 1,562,900 to 1,563,931
fliM
Shewana3_1350: fliN - flagellar motor switch protein (RefSeq), at 1,563,944 to 1,564,318
fliN
Shewana3_1351: Shewana3_1351 - flagellar biosynthesis protein, FliO (RefSeq), at 1,564,352 to 1,564,759
_1351
Shewana3_1352: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,564,756 to 1,565,502
fliP
Position (kb) 1562
1563
1564 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1562.002 kb on - strand, within Shewana3_1347 at 1562.017 kb on + strand, within Shewana3_1347 at 1562.468 kb on + strand, within fliL at 1562.705 kb on + strand, within fliL at 1563.175 kb on + strand, within fliM at 1563.241 kb on - strand, within fliM at 1563.608 kb on - strand, within fliM at 1563.635 kb on + strand, within fliM at 1563.692 kb on + strand, within fliM at 1563.961 kb on - strand at 1563.961 kb on - strand at 1563.961 kb on - strand at 1564.005 kb on - strand, within fliN at 1564.005 kb on - strand, within fliN at 1564.013 kb on - strand, within fliN at 1564.076 kb on + strand, within fliN at 1564.171 kb on - strand, within fliN at 1564.241 kb on + strand, within fliN at 1564.337 kb on + strand at 1564.337 kb on + strand at 1564.349 kb on + strand at 1564.415 kb on + strand, within Shewana3_1351 at 1564.558 kb on + strand, within Shewana3_1351 at 1564.731 kb on + strand at 1564.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,562,002 - Shewana3_1347 0.86 +1.6 1,562,017 + Shewana3_1347 0.86 -4.1 1,562,468 + fliL Shewana3_1348 0.25 -3.9 1,562,705 + fliL Shewana3_1348 0.70 -2.2 1,563,175 + fliM Shewana3_1349 0.27 -1.9 1,563,241 - fliM Shewana3_1349 0.33 +1.6 1,563,608 - fliM Shewana3_1349 0.69 +1.6 1,563,635 + fliM Shewana3_1349 0.71 -5.3 1,563,692 + fliM Shewana3_1349 0.77 -3.5 1,563,961 - +1.6 1,563,961 - +1.6 1,563,961 - +1.6 1,564,005 - fliN Shewana3_1350 0.16 +0.6 1,564,005 - fliN Shewana3_1350 0.16 +1.6 1,564,013 - fliN Shewana3_1350 0.18 +1.6 1,564,076 + fliN Shewana3_1350 0.35 -4.1 1,564,171 - fliN Shewana3_1350 0.61 +1.6 1,564,241 + fliN Shewana3_1350 0.79 -5.6 1,564,337 + +0.1 1,564,337 + -1.2 1,564,349 + -2.3 1,564,415 + Shewana3_1351 0.15 -3.4 1,564,558 + Shewana3_1351 0.50 -0.4 1,564,731 + +0.6 1,564,783 - +1.6
Or see this region's nucleotide sequence