Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1346

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliI and Shewana3_1346 are separated by 11 nucleotidesShewana3_1346 and Shewana3_1347 are separated by 159 nucleotides Shewana3_1345: fliI - flagellum-specific ATP synthase (RefSeq), at 1,558,558 to 1,559,895 fliI Shewana3_1346: Shewana3_1346 - flagellar export protein FliJ (RefSeq), at 1,559,907 to 1,560,353 _1346 Shewana3_1347: Shewana3_1347 - flagellar hook-length control protein (RefSeq), at 1,560,513 to 1,562,252 _1347 Position (kb) 1559 1560 1561Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1559.218 kb on + strand, within fliIat 1559.248 kb on + strand, within fliIat 1559.379 kb on + strand, within fliIat 1559.503 kb on + strand, within fliIat 1559.783 kb on + strandat 1559.813 kb on + strandat 1559.844 kb on - strandat 1560.669 kb on - strandat 1560.892 kb on + strand, within Shewana3_1347at 1561.055 kb on + strand, within Shewana3_1347at 1561.239 kb on + strand, within Shewana3_1347at 1561.276 kb on - strand, within Shewana3_1347at 1561.327 kb on + strand, within Shewana3_1347

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,559,218 + fliI Shewana3_1345 0.49 -3.3
1,559,248 + fliI Shewana3_1345 0.52 -2.2
1,559,379 + fliI Shewana3_1345 0.61 -1.7
1,559,503 + fliI Shewana3_1345 0.71 -0.6
1,559,783 + +0.6
1,559,813 + -0.7
1,559,844 - -0.4
1,560,669 - -1.0
1,560,892 + Shewana3_1347 0.22 -2.2
1,561,055 + Shewana3_1347 0.31 -2.2
1,561,239 + Shewana3_1347 0.42 -2.7
1,561,276 - Shewana3_1347 0.44 +1.6
1,561,327 + Shewana3_1347 0.47 -4.7

Or see this region's nucleotide sequence