Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1324

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgC and flgD are separated by 24 nucleotidesflgD and flgE are separated by 69 nucleotidesflgE and flgF are separated by 171 nucleotidesflgF and flgG are separated by 10 nucleotides Shewana3_1322: flgC - flagellar basal body rod protein FlgC (RefSeq), at 1,534,888 to 1,535,304 flgC Shewana3_1323: flgD - flagellar basal body rod modification protein (RefSeq), at 1,535,329 to 1,536,108 flgD Shewana3_1324: flgE - flagellar hook protein FlgE (RefSeq), at 1,536,178 to 1,537,539 flgE Shewana3_1325: flgF - flagellar basal body rod protein FlgF (RefSeq), at 1,537,711 to 1,538,454 flgF Shewana3_1326: flgG - flagellar basal body rod protein FlgG (RefSeq), at 1,538,465 to 1,539,253 flgG Position (kb) 1536 1537 1538Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1535.231 kb on + strand, within flgCat 1535.267 kb on + strandat 1535.482 kb on + strand, within flgDat 1535.490 kb on + strand, within flgDat 1535.490 kb on + strand, within flgDat 1535.490 kb on - strand, within flgDat 1535.498 kb on - strand, within flgDat 1535.769 kb on - strand, within flgDat 1535.854 kb on - strand, within flgDat 1535.861 kb on + strand, within flgDat 1536.068 kb on + strandat 1536.076 kb on - strandat 1536.223 kb on + strandat 1536.255 kb on + strandat 1536.271 kb on + strandat 1536.737 kb on + strand, within flgEat 1536.744 kb on - strand, within flgEat 1536.902 kb on + strand, within flgEat 1536.983 kb on - strand, within flgEat 1536.985 kb on + strand, within flgEat 1537.191 kb on - strand, within flgEat 1537.310 kb on - strand, within flgEat 1537.314 kb on + strand, within flgEat 1537.322 kb on - strand, within flgEat 1537.322 kb on - strand, within flgEat 1537.348 kb on + strand, within flgEat 1537.376 kb on - strand, within flgEat 1537.478 kb on - strandat 1537.500 kb on + strandat 1537.512 kb on + strandat 1537.560 kb on + strandat 1537.880 kb on - strand, within flgFat 1537.885 kb on + strand, within flgFat 1537.940 kb on + strand, within flgFat 1538.002 kb on + strand, within flgFat 1538.002 kb on + strand, within flgFat 1538.203 kb on - strand, within flgFat 1538.336 kb on - strand, within flgFat 1538.501 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,535,231 + flgC Shewana3_1322 0.82 -4.8
1,535,267 + -6.2
1,535,482 + flgD Shewana3_1323 0.20 -5.6
1,535,490 + flgD Shewana3_1323 0.21 -4.6
1,535,490 + flgD Shewana3_1323 0.21 -4.7
1,535,490 - flgD Shewana3_1323 0.21 +0.6
1,535,498 - flgD Shewana3_1323 0.22 +0.6
1,535,769 - flgD Shewana3_1323 0.56 +1.6
1,535,854 - flgD Shewana3_1323 0.67 +1.6
1,535,861 + flgD Shewana3_1323 0.68 -3.6
1,536,068 + -4.4
1,536,076 - +1.6
1,536,223 + -4.4
1,536,255 + -5.2
1,536,271 + -5.7
1,536,737 + flgE Shewana3_1324 0.41 -7.3
1,536,744 - flgE Shewana3_1324 0.42 +1.6
1,536,902 + flgE Shewana3_1324 0.53 -5.8
1,536,983 - flgE Shewana3_1324 0.59 +1.6
1,536,985 + flgE Shewana3_1324 0.59 -4.6
1,537,191 - flgE Shewana3_1324 0.74 +0.6
1,537,310 - flgE Shewana3_1324 0.83 +1.6
1,537,314 + flgE Shewana3_1324 0.83 -3.9
1,537,322 - flgE Shewana3_1324 0.84 +1.6
1,537,322 - flgE Shewana3_1324 0.84 +1.6
1,537,348 + flgE Shewana3_1324 0.86 -5.0
1,537,376 - flgE Shewana3_1324 0.88 +0.6
1,537,478 - +1.6
1,537,500 + -4.6
1,537,512 + -6.5
1,537,560 + -1.0
1,537,880 - flgF Shewana3_1325 0.23 -0.7
1,537,885 + flgF Shewana3_1325 0.23 -4.2
1,537,940 + flgF Shewana3_1325 0.31 -5.7
1,538,002 + flgF Shewana3_1325 0.39 -3.2
1,538,002 + flgF Shewana3_1325 0.39 -3.7
1,538,203 - flgF Shewana3_1325 0.66 +0.0
1,538,336 - flgF Shewana3_1325 0.84 +1.6
1,538,501 + -4.9

Or see this region's nucleotide sequence