Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgC and flgD are separated by 24 nucleotides flgD and flgE are separated by 69 nucleotides flgE and flgF are separated by 171 nucleotides flgF and flgG are separated by 10 nucleotides
Shewana3_1322: flgC - flagellar basal body rod protein FlgC (RefSeq), at 1,534,888 to 1,535,304
flgC
Shewana3_1323: flgD - flagellar basal body rod modification protein (RefSeq), at 1,535,329 to 1,536,108
flgD
Shewana3_1324: flgE - flagellar hook protein FlgE (RefSeq), at 1,536,178 to 1,537,539
flgE
Shewana3_1325: flgF - flagellar basal body rod protein FlgF (RefSeq), at 1,537,711 to 1,538,454
flgF
Shewana3_1326: flgG - flagellar basal body rod protein FlgG (RefSeq), at 1,538,465 to 1,539,253
flgG
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1535.231 kb on + strand, within flgC at 1535.267 kb on + strand at 1535.482 kb on + strand, within flgD at 1535.490 kb on + strand, within flgD at 1535.490 kb on + strand, within flgD at 1535.490 kb on - strand, within flgD at 1535.498 kb on - strand, within flgD at 1535.769 kb on - strand, within flgD at 1535.854 kb on - strand, within flgD at 1535.861 kb on + strand, within flgD at 1536.068 kb on + strand at 1536.076 kb on - strand at 1536.223 kb on + strand at 1536.255 kb on + strand at 1536.271 kb on + strand at 1536.737 kb on + strand, within flgE at 1536.744 kb on - strand, within flgE at 1536.902 kb on + strand, within flgE at 1536.983 kb on - strand, within flgE at 1536.985 kb on + strand, within flgE at 1537.191 kb on - strand, within flgE at 1537.310 kb on - strand, within flgE at 1537.314 kb on + strand, within flgE at 1537.322 kb on - strand, within flgE at 1537.322 kb on - strand, within flgE at 1537.348 kb on + strand, within flgE at 1537.376 kb on - strand, within flgE at 1537.478 kb on - strand at 1537.500 kb on + strand at 1537.512 kb on + strand at 1537.560 kb on + strand at 1537.880 kb on - strand, within flgF at 1537.885 kb on + strand, within flgF at 1537.940 kb on + strand, within flgF at 1538.002 kb on + strand, within flgF at 1538.002 kb on + strand, within flgF at 1538.203 kb on - strand, within flgF at 1538.336 kb on - strand, within flgF at 1538.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,535,231 + flgC Shewana3_1322 0.82 -4.8 1,535,267 + -6.2 1,535,482 + flgD Shewana3_1323 0.20 -5.6 1,535,490 + flgD Shewana3_1323 0.21 -4.6 1,535,490 + flgD Shewana3_1323 0.21 -4.7 1,535,490 - flgD Shewana3_1323 0.21 +0.6 1,535,498 - flgD Shewana3_1323 0.22 +0.6 1,535,769 - flgD Shewana3_1323 0.56 +1.6 1,535,854 - flgD Shewana3_1323 0.67 +1.6 1,535,861 + flgD Shewana3_1323 0.68 -3.6 1,536,068 + -4.4 1,536,076 - +1.6 1,536,223 + -4.4 1,536,255 + -5.2 1,536,271 + -5.7 1,536,737 + flgE Shewana3_1324 0.41 -7.3 1,536,744 - flgE Shewana3_1324 0.42 +1.6 1,536,902 + flgE Shewana3_1324 0.53 -5.8 1,536,983 - flgE Shewana3_1324 0.59 +1.6 1,536,985 + flgE Shewana3_1324 0.59 -4.6 1,537,191 - flgE Shewana3_1324 0.74 +0.6 1,537,310 - flgE Shewana3_1324 0.83 +1.6 1,537,314 + flgE Shewana3_1324 0.83 -3.9 1,537,322 - flgE Shewana3_1324 0.84 +1.6 1,537,322 - flgE Shewana3_1324 0.84 +1.6 1,537,348 + flgE Shewana3_1324 0.86 -5.0 1,537,376 - flgE Shewana3_1324 0.88 +0.6 1,537,478 - +1.6 1,537,500 + -4.6 1,537,512 + -6.5 1,537,560 + -1.0 1,537,880 - flgF Shewana3_1325 0.23 -0.7 1,537,885 + flgF Shewana3_1325 0.23 -4.2 1,537,940 + flgF Shewana3_1325 0.31 -5.7 1,538,002 + flgF Shewana3_1325 0.39 -3.2 1,538,002 + flgF Shewana3_1325 0.39 -3.7 1,538,203 - flgF Shewana3_1325 0.66 +0.0 1,538,336 - flgF Shewana3_1325 0.84 +1.6 1,538,501 + -4.9
Or see this region's nucleotide sequence