Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0771 and Shewana3_0772 are separated by 118 nucleotides Shewana3_0772 and Shewana3_0773 overlap by 1 nucleotides Shewana3_0773 and Shewana3_0774 are separated by 144 nucleotides
Shewana3_0771: Shewana3_0771 - pantoate--beta-alanine ligase (RefSeq), at 893,716 to 894,129
_0771
Shewana3_0772: Shewana3_0772 - invasion gene expression up-regulator, SirB (RefSeq), at 894,248 to 894,643
_0772
Shewana3_0773: Shewana3_0773 - hypothetical protein (RefSeq), at 894,643 to 895,431
_0773
Shewana3_0774: Shewana3_0774 - hypothetical protein (RefSeq), at 895,576 to 896,823
_0774
Position (kb) 894
895
896 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 893.696 kb on + strand at 893.704 kb on - strand at 893.774 kb on - strand, within Shewana3_0771 at 893.808 kb on - strand, within Shewana3_0771 at 893.838 kb on + strand, within Shewana3_0771 at 893.927 kb on + strand, within Shewana3_0771 at 893.935 kb on - strand, within Shewana3_0771 at 894.021 kb on + strand, within Shewana3_0771 at 894.190 kb on + strand at 894.208 kb on + strand at 894.290 kb on + strand, within Shewana3_0772 at 894.354 kb on + strand, within Shewana3_0772 at 894.425 kb on + strand, within Shewana3_0772 at 894.462 kb on + strand, within Shewana3_0772 at 894.899 kb on + strand, within Shewana3_0773 at 894.934 kb on + strand, within Shewana3_0773 at 894.990 kb on + strand, within Shewana3_0773 at 895.101 kb on + strand, within Shewana3_0773 at 895.594 kb on + strand at 895.640 kb on + strand at 895.829 kb on + strand, within Shewana3_0774 at 896.219 kb on + strand, within Shewana3_0774 at 896.221 kb on + strand, within Shewana3_0774 at 896.252 kb on + strand, within Shewana3_0774
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 893,696 + +1.0 893,704 - -0.5 893,774 - Shewana3_0771 0.14 -0.2 893,808 - Shewana3_0771 0.22 -2.2 893,838 + Shewana3_0771 0.29 +0.0 893,927 + Shewana3_0771 0.51 +1.6 893,935 - Shewana3_0771 0.53 +0.5 894,021 + Shewana3_0771 0.74 -0.1 894,190 + -1.7 894,208 + +0.3 894,290 + Shewana3_0772 0.11 -0.1 894,354 + Shewana3_0772 0.27 -0.4 894,425 + Shewana3_0772 0.45 +0.5 894,462 + Shewana3_0772 0.54 -1.0 894,899 + Shewana3_0773 0.32 -4.0 894,934 + Shewana3_0773 0.37 -0.9 894,990 + Shewana3_0773 0.44 -2.0 895,101 + Shewana3_0773 0.58 -1.1 895,594 + -0.1 895,640 + +0.1 895,829 + Shewana3_0774 0.20 -0.8 896,219 + Shewana3_0774 0.52 -0.2 896,221 + Shewana3_0774 0.52 +0.1 896,252 + Shewana3_0774 0.54 -2.4
Or see this region's nucleotide sequence