Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0680 and Shewana3_0681 are separated by 11 nucleotides Shewana3_0681 and Shewana3_0682 are separated by 5 nucleotides Shewana3_0682 and Shewana3_0683 are separated by 16 nucleotides Shewana3_0683 and Shewana3_0684 are separated by 12 nucleotides
Shewana3_0680: Shewana3_0680 - hypothetical protein (RefSeq), at 799,114 to 799,587
_0680
Shewana3_0681: Shewana3_0681 - toluene tolerance family protein (RefSeq), at 799,599 to 800,252
_0681
Shewana3_0682: Shewana3_0682 - SpoIIAA family protein (RefSeq), at 800,258 to 800,542
_0682
Shewana3_0683: Shewana3_0683 - BolA family protein (RefSeq), at 800,559 to 800,810
_0683
Shewana3_0684: Shewana3_0684 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (RefSeq), at 800,823 to 802,082
_0684
Position (kb) 800
801 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 799.679 kb on + strand, within Shewana3_0681 at 799.687 kb on + strand, within Shewana3_0681 at 799.743 kb on + strand, within Shewana3_0681 at 799.803 kb on + strand, within Shewana3_0681 at 799.811 kb on + strand, within Shewana3_0681 at 799.845 kb on + strand, within Shewana3_0681 at 799.915 kb on + strand, within Shewana3_0681 at 800.089 kb on + strand, within Shewana3_0681 at 800.134 kb on + strand, within Shewana3_0681 at 800.234 kb on + strand at 800.234 kb on + strand at 800.461 kb on + strand, within Shewana3_0682 at 800.618 kb on + strand, within Shewana3_0683
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 799,679 + Shewana3_0681 0.12 +0.1 799,687 + Shewana3_0681 0.13 -2.6 799,743 + Shewana3_0681 0.22 +0.8 799,803 + Shewana3_0681 0.31 +1.1 799,811 + Shewana3_0681 0.32 +0.3 799,845 + Shewana3_0681 0.38 +0.3 799,915 + Shewana3_0681 0.48 -1.2 800,089 + Shewana3_0681 0.75 -1.6 800,134 + Shewana3_0681 0.82 -0.2 800,234 + +0.6 800,234 + +1.0 800,461 + Shewana3_0682 0.71 +0.7 800,618 + Shewana3_0683 0.23 -4.0
Or see this region's nucleotide sequence