Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0289 and Shewana3_0290 are separated by 17 nucleotides Shewana3_0290 and Shewana3_0291 are separated by 189 nucleotides Shewana3_0291 and Shewana3_0292 are separated by 105 nucleotides Shewana3_0292 and Shewana3_0293 are separated by 80 nucleotides
Shewana3_0289: Shewana3_0289 - hypothetical protein (RefSeq), at 321,896 to 322,477
_0289
Shewana3_0290: Shewana3_0290 - CHAP domain-containing protein (RefSeq), at 322,495 to 323,184
_0290
Shewana3_0291: Shewana3_0291 - Zn-dependent hydrolase of the beta-lactamase fold (RefSeq), at 323,374 to 324,492
_0291
Shewana3_0292: Shewana3_0292 - TetR family transcriptional regulator (RefSeq), at 324,598 to 325,209
_0292
Shewana3_0293: Shewana3_0293 - anaerobic C4-dicarboxylate transporter (RefSeq), at 325,290 to 326,633
_0293
Position (kb) 323
324
325 Strain fitness (log2 ratio) -2
-1
0
1 at 322.408 kb on + strand, within Shewana3_0289 at 322.418 kb on + strand, within Shewana3_0289 at 322.551 kb on + strand at 322.552 kb on - strand at 322.606 kb on - strand, within Shewana3_0290 at 322.638 kb on - strand, within Shewana3_0290 at 322.696 kb on - strand, within Shewana3_0290 at 322.835 kb on - strand, within Shewana3_0290 at 322.937 kb on - strand, within Shewana3_0290 at 323.017 kb on - strand, within Shewana3_0290 at 323.059 kb on + strand, within Shewana3_0290 at 323.224 kb on + strand at 323.232 kb on - strand at 323.291 kb on + strand at 323.315 kb on + strand at 323.334 kb on + strand at 323.473 kb on - strand at 323.542 kb on - strand, within Shewana3_0291 at 323.556 kb on + strand, within Shewana3_0291 at 323.556 kb on + strand, within Shewana3_0291 at 323.564 kb on - strand, within Shewana3_0291 at 323.612 kb on + strand, within Shewana3_0291 at 323.643 kb on - strand, within Shewana3_0291 at 323.729 kb on - strand, within Shewana3_0291 at 323.900 kb on + strand, within Shewana3_0291 at 324.077 kb on - strand, within Shewana3_0291 at 324.365 kb on - strand, within Shewana3_0291 at 324.810 kb on + strand, within Shewana3_0292 at 324.810 kb on + strand, within Shewana3_0292 at 325.102 kb on - strand, within Shewana3_0292 at 325.102 kb on - strand, within Shewana3_0292 at 325.103 kb on - strand, within Shewana3_0292 at 325.139 kb on + strand, within Shewana3_0292 at 325.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 322,408 + Shewana3_0289 0.88 -0.1 322,418 + Shewana3_0289 0.90 -2.0 322,551 + -2.0 322,552 - -1.3 322,606 - Shewana3_0290 0.16 -0.5 322,638 - Shewana3_0290 0.21 +0.2 322,696 - Shewana3_0290 0.29 -0.5 322,835 - Shewana3_0290 0.49 -0.5 322,937 - Shewana3_0290 0.64 -1.6 323,017 - Shewana3_0290 0.76 -0.6 323,059 + Shewana3_0290 0.82 -0.3 323,224 + -1.4 323,232 - -1.2 323,291 + -0.8 323,315 + -2.2 323,334 + -0.3 323,473 - -0.8 323,542 - Shewana3_0291 0.15 -0.3 323,556 + Shewana3_0291 0.16 -0.3 323,556 + Shewana3_0291 0.16 -0.6 323,564 - Shewana3_0291 0.17 -2.0 323,612 + Shewana3_0291 0.21 -0.5 323,643 - Shewana3_0291 0.24 +0.5 323,729 - Shewana3_0291 0.32 +0.1 323,900 + Shewana3_0291 0.47 -1.1 324,077 - Shewana3_0291 0.63 +1.2 324,365 - Shewana3_0291 0.89 +0.5 324,810 + Shewana3_0292 0.35 -2.7 324,810 + Shewana3_0292 0.35 -1.9 325,102 - Shewana3_0292 0.82 -1.9 325,102 - Shewana3_0292 0.82 -2.1 325,103 - Shewana3_0292 0.83 -1.2 325,139 + Shewana3_0292 0.88 -1.5 325,342 - -0.2
Or see this region's nucleotide sequence