Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0147 and hslO are separated by 202 nucleotides hslO and Shewana3_0149 are separated by 36 nucleotides Shewana3_0149 and Shewana3_0150 are separated by 251 nucleotides
Shewana3_0147: Shewana3_0147 - phosphoenolpyruvate carboxykinase (RefSeq), at 170,761 to 172,302
_0147
Shewana3_0148: hslO - Hsp33-like chaperonin (RefSeq), at 172,505 to 173,365
hslO
Shewana3_0149: Shewana3_0149 - RNA-binding S4 domain-containing protein (RefSeq), at 173,402 to 173,800
_0149
Shewana3_0150: Shewana3_0150 - general secretion pathway protein C (RefSeq), at 174,052 to 174,978
_0150
Position (kb) 172
173
174 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 171.622 kb on + strand, within Shewana3_0147 at 171.640 kb on - strand, within Shewana3_0147 at 171.654 kb on + strand, within Shewana3_0147 at 171.735 kb on - strand, within Shewana3_0147 at 171.958 kb on - strand, within Shewana3_0147 at 171.966 kb on - strand, within Shewana3_0147 at 172.062 kb on + strand, within Shewana3_0147 at 172.130 kb on + strand, within Shewana3_0147 at 172.224 kb on - strand at 172.252 kb on + strand at 172.270 kb on + strand at 172.309 kb on - strand at 172.479 kb on - strand at 172.491 kb on - strand at 172.596 kb on - strand, within hslO at 172.671 kb on - strand, within hslO at 172.933 kb on + strand, within hslO at 172.933 kb on + strand, within hslO at 172.951 kb on - strand, within hslO at 173.029 kb on + strand, within hslO at 173.029 kb on + strand, within hslO at 173.037 kb on - strand, within hslO at 173.092 kb on + strand, within hslO at 173.100 kb on - strand, within hslO at 173.151 kb on + strand, within hslO at 173.615 kb on + strand, within Shewana3_0149 at 173.771 kb on - strand at 173.790 kb on - strand at 173.961 kb on - strand at 174.039 kb on - strand at 174.081 kb on + strand at 174.272 kb on + strand, within Shewana3_0150 at 174.335 kb on - strand, within Shewana3_0150 at 174.335 kb on - strand, within Shewana3_0150
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 171,622 + Shewana3_0147 0.56 +0.5 171,640 - Shewana3_0147 0.57 +0.2 171,654 + Shewana3_0147 0.58 -0.3 171,735 - Shewana3_0147 0.63 -0.3 171,958 - Shewana3_0147 0.78 -0.3 171,966 - Shewana3_0147 0.78 -0.7 172,062 + Shewana3_0147 0.84 +0.0 172,130 + Shewana3_0147 0.89 +0.6 172,224 - -0.1 172,252 + +0.0 172,270 + -0.9 172,309 - -0.5 172,479 - -0.8 172,491 - +0.1 172,596 - hslO Shewana3_0148 0.11 -1.3 172,671 - hslO Shewana3_0148 0.19 -3.7 172,933 + hslO Shewana3_0148 0.50 -1.6 172,933 + hslO Shewana3_0148 0.50 -2.9 172,951 - hslO Shewana3_0148 0.52 -4.9 173,029 + hslO Shewana3_0148 0.61 -2.8 173,029 + hslO Shewana3_0148 0.61 -1.7 173,037 - hslO Shewana3_0148 0.62 -3.8 173,092 + hslO Shewana3_0148 0.68 -2.5 173,100 - hslO Shewana3_0148 0.69 -2.2 173,151 + hslO Shewana3_0148 0.75 -1.5 173,615 + Shewana3_0149 0.53 -1.2 173,771 - +0.3 173,790 - -2.2 173,961 - -0.0 174,039 - +0.5 174,081 + -2.1 174,272 + Shewana3_0150 0.24 +0.1 174,335 - Shewana3_0150 0.31 -3.1 174,335 - Shewana3_0150 0.31 -0.1
Or see this region's nucleotide sequence