Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0119 and Shewana3_0120 are separated by 129 nucleotides Shewana3_0120 and Shewana3_0121 are separated by 4 nucleotides Shewana3_0121 and Shewana3_0122 are separated by 63 nucleotides
Shewana3_0119: Shewana3_0119 - phospholipid/glycerol acyltransferase (RefSeq), at 134,716 to 135,276
_0119
Shewana3_0120: Shewana3_0120 - hypothetical protein (RefSeq), at 135,406 to 135,855
_0120
Shewana3_0121: Shewana3_0121 - hypothetical protein (RefSeq), at 135,860 to 136,660
_0121
Shewana3_0122: Shewana3_0122 - hypothetical protein (RefSeq), at 136,724 to 138,367
_0122
Position (kb) 135
136
137 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 135.591 kb on + strand, within Shewana3_0120 at 135.591 kb on + strand, within Shewana3_0120 at 135.599 kb on - strand, within Shewana3_0120 at 135.693 kb on - strand, within Shewana3_0120 at 135.936 kb on - strand at 136.301 kb on - strand, within Shewana3_0121 at 136.457 kb on - strand, within Shewana3_0121 at 136.510 kb on - strand, within Shewana3_0121 at 136.663 kb on - strand at 136.721 kb on + strand at 136.729 kb on - strand at 136.923 kb on - strand, within Shewana3_0122 at 137.026 kb on - strand, within Shewana3_0122 at 137.085 kb on + strand, within Shewana3_0122 at 137.319 kb on - strand, within Shewana3_0122 at 137.421 kb on - strand, within Shewana3_0122 at 137.640 kb on + strand, within Shewana3_0122
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 135,591 + Shewana3_0120 0.41 -0.9 135,591 + Shewana3_0120 0.41 -0.9 135,599 - Shewana3_0120 0.43 -0.1 135,693 - Shewana3_0120 0.64 -1.2 135,936 - -0.9 136,301 - Shewana3_0121 0.55 -2.1 136,457 - Shewana3_0121 0.75 +0.3 136,510 - Shewana3_0121 0.81 -1.0 136,663 - -0.3 136,721 + -1.5 136,729 - +0.1 136,923 - Shewana3_0122 0.12 -3.1 137,026 - Shewana3_0122 0.18 -0.3 137,085 + Shewana3_0122 0.22 -2.4 137,319 - Shewana3_0122 0.36 -0.2 137,421 - Shewana3_0122 0.42 -0.3 137,640 + Shewana3_0122 0.56 -0.2
Or see this region's nucleotide sequence