Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0062 and Shewana3_0063 are separated by 99 nucleotides Shewana3_0063 and Shewana3_0064 are separated by 24 nucleotides Shewana3_0064 and Shewana3_0065 are separated by 88 nucleotides
Shewana3_0062: Shewana3_0062 - hypothetical protein (RefSeq), at 72,956 to 73,693
_0062
Shewana3_0063: Shewana3_0063 - NLP/P60 protein (RefSeq), at 73,793 to 74,272
_0063
Shewana3_0064: Shewana3_0064 - major facilitator transporter (RefSeq), at 74,297 to 75,745
_0064
Shewana3_0065: Shewana3_0065 - LacI family transcription regulator (RefSeq), at 75,834 to 76,862
_0065
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.312 kb on - strand, within Shewana3_0062 at 73.458 kb on - strand, within Shewana3_0062 at 73.573 kb on - strand, within Shewana3_0062 at 73.693 kb on + strand at 73.797 kb on + strand at 73.843 kb on - strand, within Shewana3_0063 at 73.870 kb on + strand, within Shewana3_0063 at 73.870 kb on + strand, within Shewana3_0063 at 73.899 kb on - strand, within Shewana3_0063 at 74.035 kb on + strand, within Shewana3_0063 at 74.112 kb on - strand, within Shewana3_0063 at 74.285 kb on + strand at 74.293 kb on - strand at 74.301 kb on - strand at 74.330 kb on + strand at 74.481 kb on + strand, within Shewana3_0064 at 74.573 kb on - strand, within Shewana3_0064 at 74.733 kb on - strand, within Shewana3_0064 at 74.773 kb on - strand, within Shewana3_0064 at 74.962 kb on + strand, within Shewana3_0064 at 74.989 kb on - strand, within Shewana3_0064 at 75.005 kb on + strand, within Shewana3_0064 at 75.011 kb on + strand, within Shewana3_0064 at 75.019 kb on - strand, within Shewana3_0064 at 75.026 kb on + strand, within Shewana3_0064 at 75.045 kb on + strand, within Shewana3_0064 at 75.133 kb on + strand, within Shewana3_0064 at 75.300 kb on - strand, within Shewana3_0064 at 75.338 kb on + strand, within Shewana3_0064 at 75.369 kb on + strand, within Shewana3_0064 at 75.382 kb on - strand, within Shewana3_0064 at 75.425 kb on + strand, within Shewana3_0064 at 75.425 kb on + strand, within Shewana3_0064 at 75.437 kb on + strand, within Shewana3_0064 at 75.548 kb on + strand, within Shewana3_0064 at 75.556 kb on - strand, within Shewana3_0064 at 75.764 kb on + strand at 75.764 kb on + strand at 75.764 kb on + strand at 75.772 kb on - strand at 75.972 kb on - strand, within Shewana3_0065 at 76.018 kb on - strand, within Shewana3_0065 at 76.054 kb on + strand, within Shewana3_0065 at 76.054 kb on + strand, within Shewana3_0065 at 76.121 kb on - strand, within Shewana3_0065 at 76.327 kb on + strand, within Shewana3_0065 at 76.461 kb on + strand, within Shewana3_0065 at 76.461 kb on + strand, within Shewana3_0065 at 76.504 kb on + strand, within Shewana3_0065 at 76.506 kb on - strand, within Shewana3_0065 at 76.510 kb on + strand, within Shewana3_0065 at 76.594 kb on + strand, within Shewana3_0065 at 76.743 kb on - strand, within Shewana3_0065
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 73,312 - Shewana3_0062 0.48 -1.6 73,458 - Shewana3_0062 0.68 -1.4 73,573 - Shewana3_0062 0.84 -0.9 73,693 + -0.0 73,797 + +0.3 73,843 - Shewana3_0063 0.10 +1.9 73,870 + Shewana3_0063 0.16 -0.9 73,870 + Shewana3_0063 0.16 -0.3 73,899 - Shewana3_0063 0.22 -1.0 74,035 + Shewana3_0063 0.50 -0.8 74,112 - Shewana3_0063 0.66 -0.4 74,285 + -0.1 74,293 - -0.0 74,301 - +0.3 74,330 + -0.2 74,481 + Shewana3_0064 0.13 -1.9 74,573 - Shewana3_0064 0.19 -0.7 74,733 - Shewana3_0064 0.30 +0.2 74,773 - Shewana3_0064 0.33 +0.3 74,962 + Shewana3_0064 0.46 -1.1 74,989 - Shewana3_0064 0.48 -0.2 75,005 + Shewana3_0064 0.49 +0.0 75,011 + Shewana3_0064 0.49 -0.5 75,019 - Shewana3_0064 0.50 -2.5 75,026 + Shewana3_0064 0.50 -1.2 75,045 + Shewana3_0064 0.52 -0.5 75,133 + Shewana3_0064 0.58 +0.2 75,300 - Shewana3_0064 0.69 +0.1 75,338 + Shewana3_0064 0.72 -2.4 75,369 + Shewana3_0064 0.74 -1.0 75,382 - Shewana3_0064 0.75 -0.4 75,425 + Shewana3_0064 0.78 -2.7 75,425 + Shewana3_0064 0.78 +0.2 75,437 + Shewana3_0064 0.79 -0.6 75,548 + Shewana3_0064 0.86 +0.2 75,556 - Shewana3_0064 0.87 -1.3 75,764 + -0.1 75,764 + -0.1 75,764 + -0.5 75,772 - -1.1 75,972 - Shewana3_0065 0.13 -0.0 76,018 - Shewana3_0065 0.18 -1.4 76,054 + Shewana3_0065 0.21 -2.6 76,054 + Shewana3_0065 0.21 -0.6 76,121 - Shewana3_0065 0.28 -1.3 76,327 + Shewana3_0065 0.48 +0.2 76,461 + Shewana3_0065 0.61 -0.8 76,461 + Shewana3_0065 0.61 -0.7 76,504 + Shewana3_0065 0.65 +0.2 76,506 - Shewana3_0065 0.65 -0.7 76,510 + Shewana3_0065 0.66 -0.4 76,594 + Shewana3_0065 0.74 -0.5 76,743 - Shewana3_0065 0.88 -0.3
Or see this region's nucleotide sequence