Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0060 and Shewana3_0061 are separated by 228 nucleotides Shewana3_0061 and Shewana3_0062 are separated by 134 nucleotides Shewana3_0062 and Shewana3_0063 are separated by 99 nucleotides
Shewana3_0060: Shewana3_0060 - ATPase domain-containing protein (RefSeq), at 70,620 to 71,666
_0060
Shewana3_0061: Shewana3_0061 - pirin domain-containing protein (RefSeq), at 71,895 to 72,821
_0061
Shewana3_0062: Shewana3_0062 - hypothetical protein (RefSeq), at 72,956 to 73,693
_0062
Shewana3_0063: Shewana3_0063 - NLP/P60 protein (RefSeq), at 73,793 to 74,272
_0063
Position (kb) 71
72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 70.907 kb on + strand, within Shewana3_0060 at 70.915 kb on - strand, within Shewana3_0060 at 71.019 kb on - strand, within Shewana3_0060 at 71.034 kb on + strand, within Shewana3_0060 at 71.185 kb on - strand, within Shewana3_0060 at 71.495 kb on - strand, within Shewana3_0060 at 71.549 kb on - strand, within Shewana3_0060 at 71.571 kb on + strand at 71.708 kb on + strand at 71.710 kb on - strand at 71.937 kb on + strand at 71.937 kb on + strand at 72.209 kb on + strand, within Shewana3_0061 at 72.217 kb on - strand, within Shewana3_0061 at 72.276 kb on + strand, within Shewana3_0061 at 72.420 kb on - strand, within Shewana3_0061 at 72.426 kb on + strand, within Shewana3_0061 at 72.506 kb on - strand, within Shewana3_0061 at 72.551 kb on - strand, within Shewana3_0061 at 72.551 kb on - strand, within Shewana3_0061 at 72.579 kb on - strand, within Shewana3_0061 at 72.632 kb on - strand, within Shewana3_0061 at 72.846 kb on - strand at 72.928 kb on - strand at 73.158 kb on + strand, within Shewana3_0062 at 73.159 kb on + strand, within Shewana3_0062 at 73.270 kb on - strand, within Shewana3_0062 at 73.312 kb on - strand, within Shewana3_0062 at 73.458 kb on - strand, within Shewana3_0062 at 73.573 kb on - strand, within Shewana3_0062 at 73.693 kb on + strand at 73.797 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 70,907 + Shewana3_0060 0.27 +0.1 70,915 - Shewana3_0060 0.28 -0.2 71,019 - Shewana3_0060 0.38 -1.1 71,034 + Shewana3_0060 0.40 +0.0 71,185 - Shewana3_0060 0.54 -1.7 71,495 - Shewana3_0060 0.84 -0.9 71,549 - Shewana3_0060 0.89 -2.0 71,571 + -0.2 71,708 + +0.6 71,710 - -1.0 71,937 + +0.0 71,937 + -0.8 72,209 + Shewana3_0061 0.34 -0.9 72,217 - Shewana3_0061 0.35 -0.1 72,276 + Shewana3_0061 0.41 +0.3 72,420 - Shewana3_0061 0.57 -0.9 72,426 + Shewana3_0061 0.57 -0.3 72,506 - Shewana3_0061 0.66 +0.1 72,551 - Shewana3_0061 0.71 +0.8 72,551 - Shewana3_0061 0.71 +0.1 72,579 - Shewana3_0061 0.74 +0.3 72,632 - Shewana3_0061 0.80 +0.3 72,846 - +0.3 72,928 - -3.2 73,158 + Shewana3_0062 0.27 +0.3 73,159 + Shewana3_0062 0.28 +0.2 73,270 - Shewana3_0062 0.43 -1.3 73,312 - Shewana3_0062 0.48 -1.6 73,458 - Shewana3_0062 0.68 -1.4 73,573 - Shewana3_0062 0.84 -0.9 73,693 + -0.0 73,797 + +0.3
Or see this region's nucleotide sequence