Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0002 and Shewana3_R0003 are separated by 126 nucleotides Shewana3_R0003 and Shewana3_R0004 are separated by 27 nucleotides Shewana3_R0004 and Shewana3_0046 are separated by 305 nucleotides Shewana3_0046 and Shewana3_0047 are separated by 21 nucleotides Shewana3_0047 and Shewana3_0048 overlap by 26 nucleotides
Shewana3_R0002: Shewana3_R0002 - 23S ribosomal RNA (RefSeq), at 52,804 to 55,698
_R0002
Shewana3_R0003: Shewana3_R0003 - 5S ribosomal RNA (RefSeq), at 55,825 to 55,944
_R0003
Shewana3_R0004: Shewana3_R0004 - tRNA-Asp (RefSeq), at 55,972 to 56,048
_R0004
Shewana3_0046: Shewana3_0046 - hypothetical protein (RefSeq), at 56,354 to 56,608
_0046
Shewana3_0047: Shewana3_0047 - BadM/Rrf2 family transcriptional regulator (RefSeq), at 56,630 to 57,085
_0047
Shewana3_0048: Shewana3_0048 - protein of unknown function DUF610, YibQ (RefSeq), at 57,060 to 57,815
_0048
Position (kb) 56
57 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.283 kb on + strand at 56.291 kb on - strand at 56.309 kb on - strand at 56.501 kb on - strand, within Shewana3_0046 at 56.540 kb on - strand, within Shewana3_0046 at 56.551 kb on + strand, within Shewana3_0046 at 56.553 kb on + strand, within Shewana3_0046 at 56.652 kb on + strand at 57.215 kb on + strand, within Shewana3_0048 at 57.223 kb on - strand, within Shewana3_0048 at 57.283 kb on - strand, within Shewana3_0048 at 57.401 kb on + strand, within Shewana3_0048 at 57.403 kb on - strand, within Shewana3_0048 at 57.431 kb on - strand, within Shewana3_0048 at 57.569 kb on - strand, within Shewana3_0048
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 56,283 + -2.6 56,291 - +0.9 56,309 - -0.2 56,501 - Shewana3_0046 0.58 +1.9 56,540 - Shewana3_0046 0.73 +0.3 56,551 + Shewana3_0046 0.77 -1.7 56,553 + Shewana3_0046 0.78 -2.4 56,652 + -2.3 57,215 + Shewana3_0048 0.21 +0.1 57,223 - Shewana3_0048 0.22 -1.8 57,283 - Shewana3_0048 0.29 +1.9 57,401 + Shewana3_0048 0.45 -1.1 57,403 - Shewana3_0048 0.45 +0.9 57,431 - Shewana3_0048 0.49 +1.5 57,569 - Shewana3_0048 0.67 -1.3
Or see this region's nucleotide sequence