Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4317 and Shewana3_4318 are separated by 113 nucleotides Shewana3_4318 and Shewana3_4319 are separated by 2 nucleotides Shewana3_4319 and Shewana3_4320 are separated by 125 nucleotides
Shewana3_4317: Shewana3_4317 - chromosome segregation ATPases-like protein (RefSeq), at 205,173 to 206,222
_4317
Shewana3_4318: Shewana3_4318 - transposase Tn3 family protein (RefSeq), at 206,336 to 209,230
_4318
Shewana3_4319: Shewana3_4319 - resolvase domain-containing protein (RefSeq), at 209,233 to 209,793
_4319
Shewana3_4320: Shewana3_4320 - signal peptidase II (RefSeq), at 209,919 to 210,998
_4320
Position (kb) 206
207
208
209
210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 205.379 kb on + strand, within Shewana3_4317 at 205.521 kb on + strand, within Shewana3_4317 at 205.521 kb on - strand, within Shewana3_4317 at 205.569 kb on + strand, within Shewana3_4317 at 205.569 kb on + strand, within Shewana3_4317 at 205.709 kb on + strand, within Shewana3_4317 at 205.828 kb on - strand, within Shewana3_4317 at 205.831 kb on + strand, within Shewana3_4317 at 205.848 kb on - strand, within Shewana3_4317 at 205.906 kb on - strand, within Shewana3_4317 at 205.916 kb on - strand, within Shewana3_4317 at 205.952 kb on + strand, within Shewana3_4317 at 206.032 kb on - strand, within Shewana3_4317 at 206.134 kb on - strand at 206.404 kb on + strand at 206.562 kb on - strand at 206.562 kb on - strand at 206.631 kb on + strand, within Shewana3_4318 at 206.636 kb on + strand, within Shewana3_4318 at 206.644 kb on - strand, within Shewana3_4318 at 206.658 kb on + strand, within Shewana3_4318 at 206.659 kb on - strand, within Shewana3_4318 at 206.707 kb on - strand, within Shewana3_4318 at 206.954 kb on - strand, within Shewana3_4318 at 207.013 kb on - strand, within Shewana3_4318 at 207.048 kb on + strand, within Shewana3_4318 at 207.160 kb on + strand, within Shewana3_4318 at 207.238 kb on + strand, within Shewana3_4318 at 207.306 kb on - strand, within Shewana3_4318 at 207.353 kb on - strand, within Shewana3_4318 at 207.353 kb on - strand, within Shewana3_4318 at 207.411 kb on + strand, within Shewana3_4318 at 207.419 kb on - strand, within Shewana3_4318 at 207.436 kb on + strand, within Shewana3_4318 at 207.444 kb on - strand, within Shewana3_4318 at 207.737 kb on - strand, within Shewana3_4318 at 207.795 kb on + strand, within Shewana3_4318 at 207.832 kb on + strand, within Shewana3_4318 at 207.870 kb on - strand, within Shewana3_4318 at 207.876 kb on + strand, within Shewana3_4318 at 207.928 kb on + strand, within Shewana3_4318 at 208.219 kb on + strand, within Shewana3_4318 at 208.466 kb on + strand, within Shewana3_4318 at 208.471 kb on + strand, within Shewana3_4318 at 208.479 kb on - strand, within Shewana3_4318 at 208.507 kb on + strand, within Shewana3_4318 at 208.542 kb on + strand, within Shewana3_4318 at 208.608 kb on + strand, within Shewana3_4318 at 208.608 kb on + strand, within Shewana3_4318 at 208.616 kb on - strand, within Shewana3_4318 at 208.803 kb on + strand, within Shewana3_4318 at 208.977 kb on + strand at 208.985 kb on - strand at 209.038 kb on + strand at 209.163 kb on + strand at 209.381 kb on + strand, within Shewana3_4319 at 209.472 kb on + strand, within Shewana3_4319 at 209.608 kb on + strand, within Shewana3_4319 at 209.723 kb on - strand, within Shewana3_4319 at 209.764 kb on - strand at 209.767 kb on + strand at 209.898 kb on + strand at 209.905 kb on + strand at 209.918 kb on - strand at 209.925 kb on - strand at 210.099 kb on + strand, within Shewana3_4320 at 210.177 kb on - strand, within Shewana3_4320 at 210.190 kb on + strand, within Shewana3_4320
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 205,379 + Shewana3_4317 0.20 -4.0 205,521 + Shewana3_4317 0.33 +0.3 205,521 - Shewana3_4317 0.33 -1.8 205,569 + Shewana3_4317 0.38 -0.1 205,569 + Shewana3_4317 0.38 -0.4 205,709 + Shewana3_4317 0.51 -0.9 205,828 - Shewana3_4317 0.62 -0.5 205,831 + Shewana3_4317 0.63 -2.9 205,848 - Shewana3_4317 0.64 -0.4 205,906 - Shewana3_4317 0.70 -0.2 205,916 - Shewana3_4317 0.71 +2.4 205,952 + Shewana3_4317 0.74 -1.4 206,032 - Shewana3_4317 0.82 +3.4 206,134 - -0.2 206,404 + +0.3 206,562 - -0.0 206,562 - +0.0 206,631 + Shewana3_4318 0.10 -0.6 206,636 + Shewana3_4318 0.10 +0.2 206,644 - Shewana3_4318 0.11 -1.2 206,658 + Shewana3_4318 0.11 -0.3 206,659 - Shewana3_4318 0.11 -0.4 206,707 - Shewana3_4318 0.13 +0.7 206,954 - Shewana3_4318 0.21 +0.2 207,013 - Shewana3_4318 0.23 +0.3 207,048 + Shewana3_4318 0.25 -2.2 207,160 + Shewana3_4318 0.28 +1.3 207,238 + Shewana3_4318 0.31 -0.4 207,306 - Shewana3_4318 0.34 +0.8 207,353 - Shewana3_4318 0.35 -2.1 207,353 - Shewana3_4318 0.35 -2.1 207,411 + Shewana3_4318 0.37 +0.6 207,419 - Shewana3_4318 0.37 +0.7 207,436 + Shewana3_4318 0.38 +0.3 207,444 - Shewana3_4318 0.38 -1.9 207,737 - Shewana3_4318 0.48 +0.3 207,795 + Shewana3_4318 0.50 -0.4 207,832 + Shewana3_4318 0.52 -1.0 207,870 - Shewana3_4318 0.53 +0.4 207,876 + Shewana3_4318 0.53 +0.9 207,928 + Shewana3_4318 0.55 +0.4 208,219 + Shewana3_4318 0.65 -1.8 208,466 + Shewana3_4318 0.74 +0.2 208,471 + Shewana3_4318 0.74 +0.1 208,479 - Shewana3_4318 0.74 -0.9 208,507 + Shewana3_4318 0.75 -0.1 208,542 + Shewana3_4318 0.76 +0.4 208,608 + Shewana3_4318 0.78 -1.7 208,608 + Shewana3_4318 0.78 +0.5 208,616 - Shewana3_4318 0.79 +0.2 208,803 + Shewana3_4318 0.85 -0.6 208,977 + -0.8 208,985 - -3.2 209,038 + -1.9 209,163 + -1.1 209,381 + Shewana3_4319 0.26 +0.1 209,472 + Shewana3_4319 0.43 -0.3 209,608 + Shewana3_4319 0.67 -1.3 209,723 - Shewana3_4319 0.87 -2.5 209,764 - -0.5 209,767 + -1.6 209,898 + -0.7 209,905 + +0.5 209,918 - -0.6 209,925 - -0.3 210,099 + Shewana3_4320 0.17 -0.4 210,177 - Shewana3_4320 0.24 +0.6 210,190 + Shewana3_4320 0.25 +0.1
Or see this region's nucleotide sequence