Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4221 and Shewana3_4222 are separated by 14 nucleotides Shewana3_4222 and Shewana3_4223 are separated by 259 nucleotides Shewana3_4223 and Shewana3_4224 are separated by 120 nucleotides Shewana3_4224 and Shewana3_4225 are separated by 623 nucleotides
Shewana3_4221: Shewana3_4221 - hypothetical protein (RefSeq), at 93,556 to 94,818
_4221
Shewana3_4222: Shewana3_4222 - hypothetical protein (RefSeq), at 94,833 to 95,180
_4222
Shewana3_4223: Shewana3_4223 - hypothetical protein (RefSeq), at 95,440 to 96,366
_4223
Shewana3_4224: Shewana3_4224 - hypothetical protein (RefSeq), at 96,487 to 96,696
_4224
Shewana3_4225: Shewana3_4225 - hypothetical protein (RefSeq), at 97,320 to 98,393
_4225
Position (kb)
95
96
97 Strain fitness (log2 ratio)
-2
-1
0
1 at 94.492 kb on - strand, within Shewana3_4221 at 94.492 kb on - strand, within Shewana3_4221 at 94.769 kb on - strand at 94.852 kb on - strand at 95.025 kb on - strand, within Shewana3_4222 at 95.581 kb on + strand, within Shewana3_4223 at 95.705 kb on + strand, within Shewana3_4223 at 96.864 kb on + strand at 96.864 kb on + strand at 97.205 kb on + strand at 97.205 kb on + strand at 97.213 kb on - strand at 97.252 kb on - strand at 97.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 94,492 - Shewana3_4221 0.74 -0.3 94,492 - Shewana3_4221 0.74 -0.1 94,769 - +1.4 94,852 - -0.6 95,025 - Shewana3_4222 0.55 +1.4 95,581 + Shewana3_4223 0.15 -1.8 95,705 + Shewana3_4223 0.29 +1.4 96,864 + +1.4 96,864 + +0.2 97,205 + -0.9 97,205 + -2.0 97,213 - -2.3 97,252 - +0.1 97,292 - -0.5
Or see this region's nucleotide sequence