Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4151 and Shewana3_4152 are separated by 41 nucleotides Shewana3_4152 and Shewana3_4153 are separated by 4 nucleotides Shewana3_4153 and Shewana3_4154 overlap by 4 nucleotides Shewana3_4154 and Shewana3_4155 are separated by 74 nucleotides
Shewana3_4151: Shewana3_4151 - hypothetical protein (RefSeq), at 12,218 to 12,505
_4151
Shewana3_4152: Shewana3_4152 - lipoprotein signal peptidase-like protein (RefSeq), at 12,547 to 12,888
_4152
Shewana3_4153: Shewana3_4153 - nitrogen regulatory protein P-II (RefSeq), at 12,893 to 13,294
_4153
Shewana3_4154: Shewana3_4154 - CzcA family heavy metal efflux protein (RefSeq), at 13,291 to 16,431
_4154
Shewana3_4155: Shewana3_4155 - RND family efflux transporter MFP subunit (RefSeq), at 16,506 to 17,447
_4155
Position (kb) 13
14
15
16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 12.292 kb on - strand, within Shewana3_4151 at 12.542 kb on - strand at 12.666 kb on - strand, within Shewana3_4152 at 12.717 kb on - strand, within Shewana3_4152 at 12.941 kb on - strand, within Shewana3_4153 at 13.038 kb on - strand, within Shewana3_4153 at 13.066 kb on + strand, within Shewana3_4153 at 13.090 kb on + strand, within Shewana3_4153 at 13.356 kb on + strand at 13.392 kb on + strand at 13.482 kb on - strand at 13.710 kb on + strand, within Shewana3_4154 at 13.803 kb on + strand, within Shewana3_4154 at 13.841 kb on - strand, within Shewana3_4154 at 14.007 kb on + strand, within Shewana3_4154 at 14.034 kb on + strand, within Shewana3_4154 at 14.099 kb on + strand, within Shewana3_4154 at 14.440 kb on - strand, within Shewana3_4154 at 14.802 kb on - strand, within Shewana3_4154 at 14.839 kb on + strand, within Shewana3_4154 at 14.917 kb on - strand, within Shewana3_4154 at 14.917 kb on - strand, within Shewana3_4154 at 14.917 kb on - strand, within Shewana3_4154 at 14.965 kb on + strand, within Shewana3_4154 at 14.967 kb on + strand, within Shewana3_4154 at 15.004 kb on - strand, within Shewana3_4154 at 15.022 kb on + strand, within Shewana3_4154 at 15.322 kb on + strand, within Shewana3_4154 at 15.330 kb on - strand, within Shewana3_4154 at 15.330 kb on - strand, within Shewana3_4154 at 15.330 kb on - strand, within Shewana3_4154 at 15.405 kb on - strand, within Shewana3_4154 at 15.405 kb on - strand, within Shewana3_4154 at 15.405 kb on - strand, within Shewana3_4154 at 15.429 kb on + strand, within Shewana3_4154 at 15.437 kb on - strand, within Shewana3_4154 at 15.439 kb on + strand, within Shewana3_4154 at 15.541 kb on - strand, within Shewana3_4154 at 15.556 kb on + strand, within Shewana3_4154 at 15.556 kb on + strand, within Shewana3_4154 at 15.568 kb on + strand, within Shewana3_4154 at 15.568 kb on + strand, within Shewana3_4154 at 15.568 kb on + strand, within Shewana3_4154 at 15.845 kb on + strand, within Shewana3_4154 at 15.940 kb on + strand, within Shewana3_4154 at 15.948 kb on - strand, within Shewana3_4154 at 15.948 kb on - strand, within Shewana3_4154 at 16.288 kb on - strand at 16.335 kb on - strand at 16.369 kb on - strand at 16.391 kb on - strand at 16.427 kb on - strand at 16.453 kb on - strand at 16.569 kb on + strand at 16.602 kb on - strand, within Shewana3_4155 at 16.921 kb on + strand, within Shewana3_4155 at 16.951 kb on + strand, within Shewana3_4155 at 17.106 kb on + strand, within Shewana3_4155 at 17.120 kb on - strand, within Shewana3_4155 at 17.294 kb on + strand, within Shewana3_4155 at 17.302 kb on - strand, within Shewana3_4155 at 17.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 12,292 - Shewana3_4151 0.26 -1.0 12,542 - -2.8 12,666 - Shewana3_4152 0.35 +0.5 12,717 - Shewana3_4152 0.50 -0.1 12,941 - Shewana3_4153 0.12 -0.8 13,038 - Shewana3_4153 0.36 -0.8 13,066 + Shewana3_4153 0.43 +0.7 13,090 + Shewana3_4153 0.49 -0.8 13,356 + -0.6 13,392 + -0.4 13,482 - +0.1 13,710 + Shewana3_4154 0.13 -0.8 13,803 + Shewana3_4154 0.16 -2.5 13,841 - Shewana3_4154 0.18 -0.3 14,007 + Shewana3_4154 0.23 +0.4 14,034 + Shewana3_4154 0.24 -0.2 14,099 + Shewana3_4154 0.26 +0.4 14,440 - Shewana3_4154 0.37 +0.2 14,802 - Shewana3_4154 0.48 +1.4 14,839 + Shewana3_4154 0.49 +1.0 14,917 - Shewana3_4154 0.52 -0.8 14,917 - Shewana3_4154 0.52 -0.2 14,917 - Shewana3_4154 0.52 -0.0 14,965 + Shewana3_4154 0.53 +1.4 14,967 + Shewana3_4154 0.53 +1.4 15,004 - Shewana3_4154 0.55 -0.6 15,022 + Shewana3_4154 0.55 +1.4 15,322 + Shewana3_4154 0.65 +0.6 15,330 - Shewana3_4154 0.65 -1.9 15,330 - Shewana3_4154 0.65 -1.6 15,330 - Shewana3_4154 0.65 -0.1 15,405 - Shewana3_4154 0.67 +0.0 15,405 - Shewana3_4154 0.67 -0.0 15,405 - Shewana3_4154 0.67 +0.7 15,429 + Shewana3_4154 0.68 +0.4 15,437 - Shewana3_4154 0.68 +0.2 15,439 + Shewana3_4154 0.68 +0.6 15,541 - Shewana3_4154 0.72 -0.5 15,556 + Shewana3_4154 0.72 -2.1 15,556 + Shewana3_4154 0.72 +2.4 15,568 + Shewana3_4154 0.72 +0.1 15,568 + Shewana3_4154 0.72 +0.4 15,568 + Shewana3_4154 0.72 -1.2 15,845 + Shewana3_4154 0.81 -1.8 15,940 + Shewana3_4154 0.84 -2.4 15,948 - Shewana3_4154 0.85 +0.0 15,948 - Shewana3_4154 0.85 -0.3 16,288 - -0.2 16,335 - -0.8 16,369 - -0.8 16,391 - -0.8 16,427 - -3.2 16,453 - +0.4 16,569 + +1.0 16,602 - Shewana3_4155 0.10 +0.5 16,921 + Shewana3_4155 0.44 +1.4 16,951 + Shewana3_4155 0.47 -0.6 17,106 + Shewana3_4155 0.64 -2.2 17,120 - Shewana3_4155 0.65 +0.7 17,294 + Shewana3_4155 0.84 -1.1 17,302 - Shewana3_4155 0.85 -2.4 17,387 + -0.8
Or see this region's nucleotide sequence