Experiment: MoLS4 with limited (C) D,L-Lactate (7mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potD-1 and potC are separated by 1 nucleotides potC and potB are separated by 2 nucleotides potB and potA overlap by 14 nucleotides
DVU0095: potD-1 - polyamine ABC transporter, periplasmic polyamine-binding protein (TIGR), at 125,677 to 126,714
potD-1
DVU0096: potC - polyamine ABC transporter, permease protein (TIGR), at 126,716 to 127,489
potC
DVU0097: potB - polyamine ABC transporter, permease protein (TIGR), at 127,492 to 128,379
potB
DVU0098: potA - polyamine ABC transporter, ATP-binding protein (TIGR), at 128,366 to 129,472
potA
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-1
0
1
2 at 126.553 kb on + strand, within potD-1 at 126.561 kb on - strand, within potD-1 at 126.592 kb on + strand, within potD-1 at 126.592 kb on + strand, within potD-1 at 126.600 kb on - strand, within potD-1 at 126.633 kb on - strand at 126.671 kb on + strand at 126.679 kb on - strand at 126.679 kb on - strand at 126.731 kb on - strand at 126.734 kb on + strand at 126.754 kb on + strand at 126.760 kb on + strand at 126.884 kb on + strand, within potC at 126.889 kb on - strand, within potC at 126.954 kb on - strand, within potC at 126.999 kb on - strand, within potC at 127.008 kb on + strand, within potC at 127.027 kb on - strand, within potC at 127.149 kb on + strand, within potC at 127.171 kb on + strand, within potC at 127.187 kb on - strand, within potC at 127.194 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.199 kb on + strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.207 kb on - strand, within potC at 127.304 kb on + strand, within potC at 127.312 kb on - strand, within potC at 127.387 kb on + strand, within potC at 127.395 kb on - strand, within potC at 127.417 kb on + strand at 127.425 kb on - strand at 127.426 kb on - strand at 127.450 kb on - strand at 127.451 kb on - strand at 127.453 kb on + strand at 127.461 kb on - strand at 127.520 kb on - strand at 127.539 kb on - strand at 127.574 kb on + strand at 127.582 kb on - strand, within potB at 127.830 kb on - strand, within potB at 127.850 kb on - strand, within potB at 127.889 kb on + strand, within potB at 127.913 kb on + strand, within potB at 127.993 kb on - strand, within potB at 128.120 kb on + strand, within potB at 128.125 kb on + strand, within potB at 128.125 kb on + strand, within potB at 128.222 kb on + strand, within potB at 128.262 kb on - strand, within potB at 128.300 kb on + strand at 128.363 kb on + strand at 128.516 kb on + strand, within potA at 128.529 kb on + strand, within potA at 128.531 kb on + strand, within potA at 128.537 kb on - strand, within potA at 128.537 kb on - strand, within potA at 128.539 kb on - strand, within potA at 128.539 kb on - strand, within potA at 128.587 kb on + strand, within potA at 128.608 kb on + strand, within potA at 128.616 kb on - strand, within potA at 128.636 kb on - strand, within potA at 128.643 kb on - strand, within potA at 128.679 kb on - strand, within potA at 128.687 kb on + strand, within potA at 128.710 kb on - strand, within potA at 128.772 kb on + strand, within potA at 128.772 kb on + strand, within potA at 128.819 kb on - strand, within potA at 128.905 kb on - strand, within potA at 128.905 kb on - strand, within potA at 128.942 kb on - strand, within potA at 128.969 kb on - strand, within potA at 128.981 kb on - strand, within potA at 128.983 kb on - strand, within potA at 129.011 kb on + strand, within potA at 129.051 kb on - strand, within potA at 129.251 kb on + strand, within potA at 129.322 kb on + strand, within potA at 129.351 kb on + strand, within potA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with limited (C) D,L-Lactate (7mM) remove 126,553 + potD-1 DVU0095 0.84 -0.3 126,561 - potD-1 DVU0095 0.85 -0.1 126,592 + potD-1 DVU0095 0.88 +0.8 126,592 + potD-1 DVU0095 0.88 +0.2 126,600 - potD-1 DVU0095 0.89 -1.1 126,633 - -0.5 126,671 + +0.2 126,679 - +0.3 126,679 - -0.0 126,731 - -0.2 126,734 + +0.2 126,754 + -0.1 126,760 + +1.2 126,884 + potC DVU0096 0.22 -0.1 126,889 - potC DVU0096 0.22 +0.7 126,954 - potC DVU0096 0.31 +0.1 126,999 - potC DVU0096 0.37 -0.1 127,008 + potC DVU0096 0.38 -0.2 127,027 - potC DVU0096 0.40 -0.3 127,149 + potC DVU0096 0.56 -0.2 127,171 + potC DVU0096 0.59 -0.6 127,187 - potC DVU0096 0.61 +0.1 127,194 + potC DVU0096 0.62 +0.3 127,199 + potC DVU0096 0.62 +0.1 127,199 + potC DVU0096 0.62 -0.3 127,199 + potC DVU0096 0.62 -1.4 127,207 - potC DVU0096 0.63 -0.0 127,207 - potC DVU0096 0.63 -0.7 127,207 - potC DVU0096 0.63 +0.2 127,207 - potC DVU0096 0.63 -0.4 127,304 + potC DVU0096 0.76 -0.1 127,312 - potC DVU0096 0.77 -0.1 127,387 + potC DVU0096 0.87 -0.3 127,395 - potC DVU0096 0.88 +0.1 127,417 + -0.1 127,425 - -0.3 127,426 - -0.5 127,450 - -0.3 127,451 - -0.2 127,453 + -0.4 127,461 - +0.0 127,520 - -0.4 127,539 - +0.6 127,574 + -0.3 127,582 - potB DVU0097 0.10 -0.0 127,830 - potB DVU0097 0.38 -0.0 127,850 - potB DVU0097 0.40 -0.2 127,889 + potB DVU0097 0.45 -0.7 127,913 + potB DVU0097 0.47 +1.1 127,993 - potB DVU0097 0.56 +0.4 128,120 + potB DVU0097 0.71 +2.4 128,125 + potB DVU0097 0.71 -1.1 128,125 + potB DVU0097 0.71 +0.9 128,222 + potB DVU0097 0.82 -0.7 128,262 - potB DVU0097 0.87 +0.1 128,300 + -0.7 128,363 + -1.2 128,516 + potA DVU0098 0.14 +0.3 128,529 + potA DVU0098 0.15 -1.7 128,531 + potA DVU0098 0.15 -1.0 128,537 - potA DVU0098 0.15 -0.1 128,537 - potA DVU0098 0.15 +0.6 128,539 - potA DVU0098 0.16 -0.3 128,539 - potA DVU0098 0.16 -0.1 128,587 + potA DVU0098 0.20 -0.3 128,608 + potA DVU0098 0.22 +0.1 128,616 - potA DVU0098 0.23 -0.3 128,636 - potA DVU0098 0.24 -0.5 128,643 - potA DVU0098 0.25 +0.0 128,679 - potA DVU0098 0.28 +0.2 128,687 + potA DVU0098 0.29 -0.1 128,710 - potA DVU0098 0.31 -0.2 128,772 + potA DVU0098 0.37 +0.0 128,772 + potA DVU0098 0.37 -0.5 128,819 - potA DVU0098 0.41 -0.6 128,905 - potA DVU0098 0.49 +0.1 128,905 - potA DVU0098 0.49 -1.2 128,942 - potA DVU0098 0.52 +0.1 128,969 - potA DVU0098 0.54 +1.8 128,981 - potA DVU0098 0.56 -0.1 128,983 - potA DVU0098 0.56 -1.0 129,011 + potA DVU0098 0.58 -0.0 129,051 - potA DVU0098 0.62 -0.5 129,251 + potA DVU0098 0.80 -0.8 129,322 + potA DVU0098 0.86 -0.0 129,351 + potA DVU0098 0.89 -0.3
Or see this region's nucleotide sequence