Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06475 and H281DRAFT_06476 are separated by 78 nucleotides H281DRAFT_06476 and H281DRAFT_06477 are separated by 54 nucleotides H281DRAFT_06477 and H281DRAFT_06478 are separated by 186 nucleotides
H281DRAFT_06475: H281DRAFT_06475 - amino acid ABC transporter membrane protein 2, PAAT family, at 12,027 to 12,740
_06475
H281DRAFT_06476: H281DRAFT_06476 - amino acid ABC transporter ATP-binding protein, PAAT family, at 12,819 to 13,610
_06476
H281DRAFT_06477: H281DRAFT_06477 - transcriptional regulator, AraC family, at 13,665 to 14,684
_06477
H281DRAFT_06478: H281DRAFT_06478 - succinylornithine aminotransferase apoenzyme, at 14,871 to 16,106
_06478
Position (kb) 13
14
15 Strain fitness (log2 ratio) -1
0
1
2 at 13.625 kb on + strand at 14.410 kb on - strand, within H281DRAFT_06477 at 14.433 kb on + strand, within H281DRAFT_06477 at 14.785 kb on + strand at 14.812 kb on + strand at 14.834 kb on + strand at 14.834 kb on + strand at 14.840 kb on + strand at 15.333 kb on + strand, within H281DRAFT_06478 at 15.381 kb on + strand, within H281DRAFT_06478 at 15.579 kb on + strand, within H281DRAFT_06478
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 13,625 + +0.2 14,410 - H281DRAFT_06477 0.73 +2.1 14,433 + H281DRAFT_06477 0.75 +0.4 14,785 + -0.7 14,812 + +0.4 14,834 + -0.1 14,834 + +0.7 14,840 + -1.7 15,333 + H281DRAFT_06478 0.37 -0.3 15,381 + H281DRAFT_06478 0.41 -0.9 15,579 + H281DRAFT_06478 0.57 +1.9
Or see this region's nucleotide sequence