Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06056 and H281DRAFT_06057 are separated by 51 nucleotides H281DRAFT_06057 and H281DRAFT_06058 are separated by 33 nucleotides H281DRAFT_06058 and H281DRAFT_06059 are separated by 124 nucleotides
H281DRAFT_06056: H281DRAFT_06056 - histidine kinase (EC 2.7.13.3), at 70,530 to 71,465
_06056
H281DRAFT_06057: H281DRAFT_06057 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA, at 71,517 to 71,858
_06057
H281DRAFT_06058: H281DRAFT_06058 - transcriptional regulator, AraC family, at 71,892 to 72,674
_06058
H281DRAFT_06059: H281DRAFT_06059 - O-succinylhomoserine sulfhydrylase, at 72,799 to 73,989
_06059
Position (kb) 71
72 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 70.653 kb on + strand, within H281DRAFT_06056 at 70.654 kb on - strand, within H281DRAFT_06056 at 70.671 kb on - strand at 70.671 kb on - strand, within H281DRAFT_06056 at 70.811 kb on + strand, within H281DRAFT_06056 at 70.811 kb on + strand, within H281DRAFT_06056 at 70.963 kb on - strand, within H281DRAFT_06056 at 70.963 kb on - strand, within H281DRAFT_06056 at 71.158 kb on + strand, within H281DRAFT_06056 at 71.159 kb on - strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.168 kb on + strand, within H281DRAFT_06056 at 71.169 kb on - strand, within H281DRAFT_06056 at 71.169 kb on - strand, within H281DRAFT_06056 at 71.206 kb on - strand, within H281DRAFT_06056 at 71.377 kb on - strand at 71.378 kb on + strand at 71.378 kb on + strand at 71.378 kb on + strand at 71.379 kb on - strand at 71.477 kb on + strand at 71.478 kb on - strand at 71.516 kb on + strand at 71.516 kb on + strand at 71.516 kb on + strand at 71.517 kb on - strand at 71.517 kb on - strand at 71.614 kb on - strand, within H281DRAFT_06057 at 71.857 kb on - strand at 71.857 kb on - strand at 72.084 kb on + strand, within H281DRAFT_06058 at 72.085 kb on - strand, within H281DRAFT_06058 at 72.198 kb on - strand, within H281DRAFT_06058 at 72.528 kb on - strand, within H281DRAFT_06058 at 72.541 kb on + strand, within H281DRAFT_06058 at 72.541 kb on + strand at 72.611 kb on + strand at 72.611 kb on + strand at 72.612 kb on - strand at 72.612 kb on - strand at 72.770 kb on + strand at 72.770 kb on + strand at 72.771 kb on - strand at 72.771 kb on - strand at 72.771 kb on - strand at 72.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 70,653 + H281DRAFT_06056 0.13 +1.4 70,654 - H281DRAFT_06056 0.13 +1.1 70,671 - +2.0 70,671 - H281DRAFT_06056 0.15 +1.4 70,811 + H281DRAFT_06056 0.30 +1.3 70,811 + H281DRAFT_06056 0.30 +1.5 70,963 - H281DRAFT_06056 0.46 +1.2 70,963 - H281DRAFT_06056 0.46 +1.4 71,158 + H281DRAFT_06056 0.67 +1.3 71,159 - H281DRAFT_06056 0.67 +1.0 71,168 + H281DRAFT_06056 0.68 +0.8 71,168 + H281DRAFT_06056 0.68 +0.9 71,168 + H281DRAFT_06056 0.68 +1.1 71,168 + H281DRAFT_06056 0.68 +0.5 71,168 + H281DRAFT_06056 0.68 +0.9 71,168 + H281DRAFT_06056 0.68 +2.0 71,169 - H281DRAFT_06056 0.68 +3.9 71,169 - H281DRAFT_06056 0.68 +1.7 71,206 - H281DRAFT_06056 0.72 +1.1 71,377 - -1.9 71,378 + -0.2 71,378 + +1.9 71,378 + +0.3 71,379 - -1.5 71,477 + -0.2 71,478 - +1.0 71,516 + -1.9 71,516 + -0.4 71,516 + -0.3 71,517 - -0.4 71,517 - -0.5 71,614 - H281DRAFT_06057 0.28 -0.2 71,857 - +0.8 71,857 - -0.6 72,084 + H281DRAFT_06058 0.25 +1.4 72,085 - H281DRAFT_06058 0.25 -0.2 72,198 - H281DRAFT_06058 0.39 +0.4 72,528 - H281DRAFT_06058 0.81 -0.4 72,541 + H281DRAFT_06058 0.83 +0.0 72,541 + +2.6 72,611 + +0.0 72,611 + +0.4 72,612 - +0.7 72,612 - -1.6 72,770 + -1.0 72,770 + -0.7 72,771 - +0.2 72,771 - -0.3 72,771 - +0.8 72,771 - -0.5
Or see this region's nucleotide sequence