Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06005 and H281DRAFT_06006 are separated by 279 nucleotides H281DRAFT_06006 and H281DRAFT_06007 are separated by 105 nucleotides H281DRAFT_06007 and H281DRAFT_06008 are separated by 632 nucleotides
H281DRAFT_06005: H281DRAFT_06005 - methyltransferase, FkbM family, at 6,078 to 6,788
_06005
H281DRAFT_06006: H281DRAFT_06006 - MEKHLA domain-containing protein, at 7,068 to 7,523
_06006
H281DRAFT_06007: H281DRAFT_06007 - amino acid ABC transporter substrate-binding protein, PAAT family, at 7,629 to 8,360
_06007
H281DRAFT_06008: H281DRAFT_06008 - RNA polymerase, sigma 70 subunit, RpoD, at 8,993 to 11,227
_06008
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.644 kb on - strand, within H281DRAFT_06005 at 6.644 kb on - strand, within H281DRAFT_06005 at 6.673 kb on - strand, within H281DRAFT_06005 at 6.712 kb on + strand, within H281DRAFT_06005 at 6.712 kb on + strand, within H281DRAFT_06005 at 6.712 kb on + strand, within H281DRAFT_06005 at 6.713 kb on - strand, within H281DRAFT_06005 at 6.713 kb on - strand, within H281DRAFT_06005 at 6.776 kb on + strand at 6.829 kb on + strand at 6.859 kb on + strand at 6.859 kb on + strand at 6.927 kb on + strand at 7.036 kb on + strand at 7.060 kb on + strand at 7.060 kb on + strand at 7.060 kb on + strand at 7.060 kb on + strand at 7.345 kb on + strand, within H281DRAFT_06006 at 7.346 kb on - strand, within H281DRAFT_06006 at 7.346 kb on - strand, within H281DRAFT_06006 at 7.436 kb on + strand, within H281DRAFT_06006 at 7.437 kb on - strand, within H281DRAFT_06006 at 7.457 kb on + strand, within H281DRAFT_06006 at 7.474 kb on + strand, within H281DRAFT_06006 at 7.478 kb on + strand at 7.478 kb on + strand at 7.479 kb on - strand at 7.479 kb on - strand at 7.479 kb on - strand at 7.479 kb on - strand at 7.528 kb on + strand at 7.529 kb on - strand at 7.627 kb on + strand at 7.628 kb on - strand at 7.714 kb on + strand, within H281DRAFT_06007 at 7.714 kb on + strand, within H281DRAFT_06007 at 7.892 kb on - strand, within H281DRAFT_06007 at 7.892 kb on - strand, within H281DRAFT_06007 at 7.923 kb on + strand, within H281DRAFT_06007 at 8.053 kb on + strand, within H281DRAFT_06007 at 8.053 kb on + strand, within H281DRAFT_06007 at 8.054 kb on - strand, within H281DRAFT_06007 at 8.054 kb on - strand, within H281DRAFT_06007 at 8.054 kb on - strand, within H281DRAFT_06007 at 8.054 kb on - strand, within H281DRAFT_06007 at 8.193 kb on + strand, within H281DRAFT_06007 at 8.193 kb on + strand, within H281DRAFT_06007 at 8.194 kb on - strand, within H281DRAFT_06007 at 8.220 kb on - strand, within H281DRAFT_06007 at 8.402 kb on + strand at 8.403 kb on - strand at 8.836 kb on - strand at 8.853 kb on + strand at 8.853 kb on + strand at 8.853 kb on + strand at 8.854 kb on - strand at 8.854 kb on - strand at 8.893 kb on + strand at 8.894 kb on - strand at 8.894 kb on - strand at 8.894 kb on - strand at 8.896 kb on + strand at 8.896 kb on + strand at 8.964 kb on + strand at 8.965 kb on - strand at 8.965 kb on - strand at 8.968 kb on + strand at 8.969 kb on - strand at 8.969 kb on - strand at 8.975 kb on + strand at 8.976 kb on - strand at 8.976 kb on - strand at 9.262 kb on + strand, within H281DRAFT_06008 at 9.263 kb on - strand, within H281DRAFT_06008
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 6,644 - H281DRAFT_06005 0.80 -1.9 6,644 - H281DRAFT_06005 0.80 -1.4 6,673 - H281DRAFT_06005 0.84 +0.1 6,712 + H281DRAFT_06005 0.89 +0.4 6,712 + H281DRAFT_06005 0.89 +1.4 6,712 + H281DRAFT_06005 0.89 -0.8 6,713 - H281DRAFT_06005 0.89 -0.4 6,713 - H281DRAFT_06005 0.89 +0.7 6,776 + -0.2 6,829 + +0.1 6,859 + +0.9 6,859 + -0.2 6,927 + +0.1 7,036 + +0.8 7,060 + +0.0 7,060 + -0.9 7,060 + -0.3 7,060 + -0.8 7,345 + H281DRAFT_06006 0.61 +0.5 7,346 - H281DRAFT_06006 0.61 -0.1 7,346 - H281DRAFT_06006 0.61 -0.6 7,436 + H281DRAFT_06006 0.81 +0.2 7,437 - H281DRAFT_06006 0.81 -1.0 7,457 + H281DRAFT_06006 0.85 -1.7 7,474 + H281DRAFT_06006 0.89 -0.7 7,478 + -0.2 7,478 + -1.0 7,479 - +1.8 7,479 - -0.1 7,479 - +0.8 7,479 - +1.0 7,528 + -0.1 7,529 - +1.2 7,627 + +0.0 7,628 - -0.1 7,714 + H281DRAFT_06007 0.12 +0.4 7,714 + H281DRAFT_06007 0.12 -0.4 7,892 - H281DRAFT_06007 0.36 -0.6 7,892 - H281DRAFT_06007 0.36 -0.6 7,923 + H281DRAFT_06007 0.40 -0.6 8,053 + H281DRAFT_06007 0.58 +0.6 8,053 + H281DRAFT_06007 0.58 +0.4 8,054 - H281DRAFT_06007 0.58 -0.7 8,054 - H281DRAFT_06007 0.58 +0.1 8,054 - H281DRAFT_06007 0.58 +1.2 8,054 - H281DRAFT_06007 0.58 -0.1 8,193 + H281DRAFT_06007 0.77 -0.3 8,193 + H281DRAFT_06007 0.77 -1.0 8,194 - H281DRAFT_06007 0.77 -0.0 8,220 - H281DRAFT_06007 0.81 +0.7 8,402 + +0.3 8,403 - +0.3 8,836 - -0.1 8,853 + +0.4 8,853 + +0.4 8,853 + +2.6 8,854 - -1.0 8,854 - -0.6 8,893 + +0.4 8,894 - -0.5 8,894 - -0.2 8,894 - +0.5 8,896 + -1.9 8,896 + +0.2 8,964 + +0.4 8,965 - -0.9 8,965 - -0.3 8,968 + +0.5 8,969 - +0.1 8,969 - +0.5 8,975 + -0.4 8,976 - -0.4 8,976 - -0.6 9,262 + H281DRAFT_06008 0.12 -1.8 9,263 - H281DRAFT_06008 0.12 -2.0
Or see this region's nucleotide sequence