Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05696 and H281DRAFT_05697 are separated by 49 nucleotides H281DRAFT_05697 and H281DRAFT_05698 are separated by 126 nucleotides H281DRAFT_05698 and H281DRAFT_05699 are separated by 290 nucleotides
H281DRAFT_05696: H281DRAFT_05696 - Xaa-Pro aminopeptidase, at 112,057 to 113,871
_05696
H281DRAFT_05697: H281DRAFT_05697 - 2-haloacid dehalogenase, at 113,921 to 114,544
_05697
H281DRAFT_05698: H281DRAFT_05698 - protein of unknown function (DUF748), at 114,671 to 115,795
_05698
H281DRAFT_05699: H281DRAFT_05699 - sulfate-transporting ATPase, at 116,086 to 117,753
_05699
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1 at 113.709 kb on - strand at 113.785 kb on + strand at 113.857 kb on - strand at 113.904 kb on + strand at 113.905 kb on - strand at 113.905 kb on - strand at 113.981 kb on + strand at 113.987 kb on + strand, within H281DRAFT_05697 at 113.988 kb on - strand, within H281DRAFT_05697 at 114.149 kb on + strand, within H281DRAFT_05697 at 114.272 kb on + strand, within H281DRAFT_05697 at 114.273 kb on - strand, within H281DRAFT_05697 at 114.420 kb on + strand, within H281DRAFT_05697 at 114.420 kb on + strand, within H281DRAFT_05697 at 114.421 kb on - strand, within H281DRAFT_05697 at 114.646 kb on + strand at 114.647 kb on - strand at 114.672 kb on + strand at 114.672 kb on + strand at 114.673 kb on - strand at 114.990 kb on + strand, within H281DRAFT_05698 at 115.071 kb on + strand, within H281DRAFT_05698 at 115.072 kb on - strand, within H281DRAFT_05698 at 115.072 kb on - strand, within H281DRAFT_05698 at 115.268 kb on + strand, within H281DRAFT_05698 at 115.281 kb on + strand, within H281DRAFT_05698 at 115.282 kb on - strand, within H281DRAFT_05698 at 115.309 kb on - strand, within H281DRAFT_05698 at 115.544 kb on - strand, within H281DRAFT_05698 at 115.708 kb on - strand at 115.873 kb on + strand at 115.873 kb on + strand at 115.874 kb on - strand at 115.874 kb on - strand at 115.875 kb on + strand at 115.875 kb on + strand at 115.875 kb on + strand at 115.875 kb on + strand at 115.876 kb on - strand at 115.876 kb on - strand at 115.876 kb on - strand at 115.888 kb on + strand at 115.889 kb on - strand at 115.945 kb on + strand at 115.946 kb on - strand at 116.094 kb on + strand at 116.174 kb on + strand at 116.174 kb on + strand at 116.175 kb on - strand at 116.175 kb on - strand at 116.193 kb on + strand at 116.474 kb on + strand, within H281DRAFT_05699 at 116.474 kb on + strand, within H281DRAFT_05699 at 116.475 kb on - strand, within H281DRAFT_05699 at 116.495 kb on + strand, within H281DRAFT_05699 at 116.495 kb on + strand, within H281DRAFT_05699 at 116.495 kb on + strand, within H281DRAFT_05699 at 116.496 kb on - strand, within H281DRAFT_05699 at 116.496 kb on - strand, within H281DRAFT_05699 at 116.585 kb on + strand, within H281DRAFT_05699 at 116.586 kb on - strand, within H281DRAFT_05699 at 116.586 kb on - strand, within H281DRAFT_05699 at 116.756 kb on + strand, within H281DRAFT_05699 at 116.756 kb on + strand, within H281DRAFT_05699 at 116.757 kb on - strand, within H281DRAFT_05699
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 113,709 - +1.0 113,785 + +0.0 113,857 - +0.6 113,904 + +0.2 113,905 - -0.8 113,905 - +0.2 113,981 + +0.6 113,987 + H281DRAFT_05697 0.11 +0.2 113,988 - H281DRAFT_05697 0.11 -0.0 114,149 + H281DRAFT_05697 0.37 +0.7 114,272 + H281DRAFT_05697 0.56 +0.9 114,273 - H281DRAFT_05697 0.56 +0.2 114,420 + H281DRAFT_05697 0.80 -0.2 114,420 + H281DRAFT_05697 0.80 -0.0 114,421 - H281DRAFT_05697 0.80 -1.3 114,646 + -0.2 114,647 - -0.0 114,672 + +0.1 114,672 + +0.2 114,673 - +0.6 114,990 + H281DRAFT_05698 0.28 +1.6 115,071 + H281DRAFT_05698 0.36 -0.2 115,072 - H281DRAFT_05698 0.36 +1.0 115,072 - H281DRAFT_05698 0.36 +1.4 115,268 + H281DRAFT_05698 0.53 -0.1 115,281 + H281DRAFT_05698 0.54 +1.5 115,282 - H281DRAFT_05698 0.54 +0.3 115,309 - H281DRAFT_05698 0.57 -0.7 115,544 - H281DRAFT_05698 0.78 -0.4 115,708 - +0.4 115,873 + +0.0 115,873 + +0.3 115,874 - -0.7 115,874 - -0.5 115,875 + -0.8 115,875 + +0.1 115,875 + +0.5 115,875 + +0.7 115,876 - +0.1 115,876 - +0.4 115,876 - +0.3 115,888 + +0.4 115,889 - -0.1 115,945 + -0.2 115,946 - -0.1 116,094 + -0.3 116,174 + +0.1 116,174 + +0.7 116,175 - +0.5 116,175 - +1.4 116,193 + -2.3 116,474 + H281DRAFT_05699 0.23 -1.9 116,474 + H281DRAFT_05699 0.23 +0.2 116,475 - H281DRAFT_05699 0.23 -0.9 116,495 + H281DRAFT_05699 0.25 -0.4 116,495 + H281DRAFT_05699 0.25 +0.1 116,495 + H281DRAFT_05699 0.25 -1.9 116,496 - H281DRAFT_05699 0.25 +1.2 116,496 - H281DRAFT_05699 0.25 -0.4 116,585 + H281DRAFT_05699 0.30 +0.5 116,586 - H281DRAFT_05699 0.30 +0.1 116,586 - H281DRAFT_05699 0.30 +0.2 116,756 + H281DRAFT_05699 0.40 +0.8 116,756 + H281DRAFT_05699 0.40 +0.2 116,757 - H281DRAFT_05699 0.40 -0.3
Or see this region's nucleotide sequence