Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05277

Experiment: LB in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05275 and H281DRAFT_05276 are separated by 12 nucleotidesH281DRAFT_05276 and H281DRAFT_05277 overlap by 1 nucleotidesH281DRAFT_05277 and H281DRAFT_05278 overlap by 1 nucleotidesH281DRAFT_05278 and H281DRAFT_05279 overlap by 4 nucleotides H281DRAFT_05275: H281DRAFT_05275 - transcriptional regulator, LysR family, at 18,037 to 18,978 _05275 H281DRAFT_05276: H281DRAFT_05276 - malonate decarboxylase epsilon subunit, at 18,991 to 19,923 _05276 H281DRAFT_05277: H281DRAFT_05277 - phosphoribosyl-dephospho-CoA transferase, at 19,923 to 20,630 _05277 H281DRAFT_05278: H281DRAFT_05278 - malonate decarboxylase gamma subunit, at 20,630 to 21,445 _05278 H281DRAFT_05279: H281DRAFT_05279 - malonate decarboxylase beta subunit, at 21,442 to 22,329 _05279 Position (kb) 19 20 21Strain fitness (log2 ratio) -2 -1 0 1at 19.185 kb on - strand, within H281DRAFT_05276at 19.473 kb on - strand, within H281DRAFT_05276at 19.473 kb on - strand, within H281DRAFT_05276at 19.571 kb on + strand, within H281DRAFT_05276at 19.809 kb on + strand, within H281DRAFT_05276at 20.778 kb on - strand, within H281DRAFT_05278at 21.021 kb on - strand, within H281DRAFT_05278at 21.093 kb on + strand, within H281DRAFT_05278at 21.093 kb on + strand, within H281DRAFT_05278at 21.094 kb on - strand, within H281DRAFT_05278at 21.489 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL
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19,185 - H281DRAFT_05276 0.21 -0.5
19,473 - H281DRAFT_05276 0.52 +0.8
19,473 - H281DRAFT_05276 0.52 +0.1
19,571 + H281DRAFT_05276 0.62 -0.7
19,809 + H281DRAFT_05276 0.88 -0.3
20,778 - H281DRAFT_05278 0.18 -0.4
21,021 - H281DRAFT_05278 0.48 -0.2
21,093 + H281DRAFT_05278 0.57 -0.4
21,093 + H281DRAFT_05278 0.57 +0.7
21,094 - H281DRAFT_05278 0.57 -2.6
21,489 - +0.7

Or see this region's nucleotide sequence