Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05074 and H281DRAFT_05075 are separated by 219 nucleotides H281DRAFT_05075 and H281DRAFT_05076 are separated by 1 nucleotides H281DRAFT_05076 and H281DRAFT_05077 overlap by 4 nucleotides H281DRAFT_05077 and H281DRAFT_05078 are separated by 143 nucleotides
H281DRAFT_05074: H281DRAFT_05074 - protease I, at 13,528 to 14,109
_05074
H281DRAFT_05075: H281DRAFT_05075 - hypothetical protein, at 14,329 to 14,805
_05075
H281DRAFT_05076: H281DRAFT_05076 - hypothetical protein, at 14,807 to 14,938
_05076
H281DRAFT_05077: H281DRAFT_05077 - transcriptional regulator, BadM/Rrf2 family, at 14,935 to 15,390
_05077
H281DRAFT_05078: H281DRAFT_05078 - hypothetical protein, at 15,534 to 16,181
_05078
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1 at 14.142 kb on + strand at 14.178 kb on + strand at 14.179 kb on - strand at 14.325 kb on - strand at 14.325 kb on - strand at 14.653 kb on - strand, within H281DRAFT_05075 at 14.653 kb on - strand, within H281DRAFT_05075 at 14.770 kb on + strand at 15.169 kb on + strand, within H281DRAFT_05077 at 15.485 kb on + strand at 15.924 kb on + strand, within H281DRAFT_05078 at 15.926 kb on + strand, within H281DRAFT_05078 at 15.927 kb on - strand, within H281DRAFT_05078 at 16.110 kb on + strand, within H281DRAFT_05078 at 16.200 kb on + strand at 16.200 kb on + strand at 16.343 kb on + strand at 16.389 kb on + strand at 16.389 kb on + strand at 16.389 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 14,142 + +0.1 14,178 + -0.2 14,179 - -0.1 14,325 - -1.0 14,325 - +0.1 14,653 - H281DRAFT_05075 0.68 -0.6 14,653 - H281DRAFT_05075 0.68 +0.2 14,770 + -0.0 15,169 + H281DRAFT_05077 0.51 -2.0 15,485 + +0.3 15,924 + H281DRAFT_05078 0.60 -0.4 15,926 + H281DRAFT_05078 0.60 +0.2 15,927 - H281DRAFT_05078 0.61 -1.0 16,110 + H281DRAFT_05078 0.89 -1.2 16,200 + +0.1 16,200 + -0.2 16,343 + -1.8 16,389 + -0.1 16,389 + -0.5 16,389 + +0.0
Or see this region's nucleotide sequence