Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04829 and H281DRAFT_04830 are separated by 9 nucleotides H281DRAFT_04830 and H281DRAFT_04831 overlap by 4 nucleotides H281DRAFT_04831 and H281DRAFT_04832 overlap by 4 nucleotides H281DRAFT_04832 and H281DRAFT_04833 overlap by 14 nucleotides
H281DRAFT_04829: H281DRAFT_04829 - Methyltransferase domain, at 263,900 to 264,649
_04829
H281DRAFT_04830: H281DRAFT_04830 - sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family, at 264,659 to 265,336
_04830
H281DRAFT_04831: H281DRAFT_04831 - hypothetical protein, at 265,333 to 266,256
_04831
H281DRAFT_04832: H281DRAFT_04832 - Acetyltransferase (GNAT) domain-containing protein, at 266,253 to 267,227
_04832
H281DRAFT_04833: H281DRAFT_04833 - WxcM-like, C-terminal, at 267,214 to 267,624
_04833
Position (kb) 265
266
267 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9
10 at 264.396 kb on + strand, within H281DRAFT_04829 at 265.448 kb on - strand at 265.630 kb on + strand, within H281DRAFT_04831 at 265.636 kb on + strand, within H281DRAFT_04831 at 265.636 kb on + strand, within H281DRAFT_04831 at 265.636 kb on + strand, within H281DRAFT_04831 at 265.639 kb on + strand, within H281DRAFT_04831 at 266.020 kb on + strand, within H281DRAFT_04831 at 266.020 kb on + strand, within H281DRAFT_04831 at 266.200 kb on + strand at 267.199 kb on + strand at 267.199 kb on + strand at 267.199 kb on + strand at 267.202 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 264,396 + H281DRAFT_04829 0.66 +8.1 265,448 - -1.3 265,630 + H281DRAFT_04831 0.32 +6.9 265,636 + H281DRAFT_04831 0.33 +4.8 265,636 + H281DRAFT_04831 0.33 +10.1 265,636 + H281DRAFT_04831 0.33 +7.2 265,639 + H281DRAFT_04831 0.33 +5.4 266,020 + H281DRAFT_04831 0.74 +4.5 266,020 + H281DRAFT_04831 0.74 +7.5 266,200 + +5.6 267,199 + -0.1 267,199 + +6.9 267,199 + -0.7 267,202 + +4.6
Or see this region's nucleotide sequence