Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04740 and H281DRAFT_04741 are separated by 31 nucleotides H281DRAFT_04741 and H281DRAFT_04742 overlap by 4 nucleotides
H281DRAFT_04740: H281DRAFT_04740 - MFS transporter, AAHS family, 4-hydroxybenzoate transporter, at 148,036 to 149,400
_04740
H281DRAFT_04741: H281DRAFT_04741 - homogentisate 1,2-dioxygenase, at 149,432 to 150,778
_04741
H281DRAFT_04742: H281DRAFT_04742 - fumarylacetoacetate hydrolase, at 150,775 to 152,076
_04742
Position (kb) 149
150
151 Strain fitness (log2 ratio) -1
0
1
2
3 at 148.460 kb on + strand, within H281DRAFT_04740 at 148.673 kb on + strand, within H281DRAFT_04740 at 148.673 kb on + strand, within H281DRAFT_04740 at 148.674 kb on - strand, within H281DRAFT_04740 at 148.870 kb on + strand, within H281DRAFT_04740 at 149.100 kb on - strand, within H281DRAFT_04740 at 149.437 kb on + strand at 149.580 kb on - strand, within H281DRAFT_04741 at 149.655 kb on - strand at 149.681 kb on - strand, within H281DRAFT_04741 at 149.907 kb on - strand, within H281DRAFT_04741 at 149.907 kb on - strand, within H281DRAFT_04741 at 150.167 kb on + strand, within H281DRAFT_04741 at 150.275 kb on + strand, within H281DRAFT_04741 at 150.276 kb on - strand, within H281DRAFT_04741 at 150.285 kb on - strand, within H281DRAFT_04741 at 150.287 kb on - strand, within H281DRAFT_04741 at 150.290 kb on + strand, within H281DRAFT_04741 at 150.291 kb on - strand, within H281DRAFT_04741 at 150.325 kb on + strand, within H281DRAFT_04741 at 150.589 kb on + strand, within H281DRAFT_04741 at 150.617 kb on - strand, within H281DRAFT_04741 at 150.725 kb on + strand at 150.725 kb on + strand at 150.728 kb on + strand at 150.728 kb on + strand at 150.748 kb on + strand at 150.748 kb on + strand at 151.080 kb on + strand, within H281DRAFT_04742 at 151.204 kb on + strand, within H281DRAFT_04742 at 151.495 kb on - strand, within H281DRAFT_04742 at 151.552 kb on + strand, within H281DRAFT_04742 at 151.553 kb on - strand, within H281DRAFT_04742 at 151.553 kb on - strand, within H281DRAFT_04742 at 151.553 kb on - strand, within H281DRAFT_04742 at 151.553 kb on - strand, within H281DRAFT_04742 at 151.678 kb on + strand, within H281DRAFT_04742 at 151.678 kb on + strand, within H281DRAFT_04742 at 151.679 kb on - strand, within H281DRAFT_04742 at 151.731 kb on - strand, within H281DRAFT_04742
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 148,460 + H281DRAFT_04740 0.31 +0.2 148,673 + H281DRAFT_04740 0.47 +0.1 148,673 + H281DRAFT_04740 0.47 +0.0 148,674 - H281DRAFT_04740 0.47 -0.4 148,870 + H281DRAFT_04740 0.61 -0.1 149,100 - H281DRAFT_04740 0.78 -0.1 149,437 + +0.2 149,580 - H281DRAFT_04741 0.11 +0.1 149,655 - +1.1 149,681 - H281DRAFT_04741 0.18 +0.0 149,907 - H281DRAFT_04741 0.35 -0.1 149,907 - H281DRAFT_04741 0.35 -0.5 150,167 + H281DRAFT_04741 0.55 +1.1 150,275 + H281DRAFT_04741 0.63 +1.6 150,276 - H281DRAFT_04741 0.63 +1.5 150,285 - H281DRAFT_04741 0.63 +0.1 150,287 - H281DRAFT_04741 0.63 +0.5 150,290 + H281DRAFT_04741 0.64 -0.7 150,291 - H281DRAFT_04741 0.64 +0.5 150,325 + H281DRAFT_04741 0.66 -0.6 150,589 + H281DRAFT_04741 0.86 -0.3 150,617 - H281DRAFT_04741 0.88 +0.5 150,725 + +0.2 150,725 + -0.3 150,728 + +1.4 150,728 + +1.0 150,748 + -0.1 150,748 + -0.5 151,080 + H281DRAFT_04742 0.23 -0.6 151,204 + H281DRAFT_04742 0.33 +3.1 151,495 - H281DRAFT_04742 0.55 +0.5 151,552 + H281DRAFT_04742 0.60 +0.3 151,553 - H281DRAFT_04742 0.60 +0.0 151,553 - H281DRAFT_04742 0.60 +0.4 151,553 - H281DRAFT_04742 0.60 +0.9 151,553 - H281DRAFT_04742 0.60 -1.1 151,678 + H281DRAFT_04742 0.69 +0.0 151,678 + H281DRAFT_04742 0.69 -0.3 151,679 - H281DRAFT_04742 0.69 +1.0 151,731 - H281DRAFT_04742 0.73 +0.3
Or see this region's nucleotide sequence