Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04717 and H281DRAFT_04718 are separated by 19 nucleotides H281DRAFT_04718 and H281DRAFT_04719 are separated by 31 nucleotides H281DRAFT_04719 and H281DRAFT_04720 are separated by 137 nucleotides
H281DRAFT_04717: H281DRAFT_04717 - tol-pal system protein YbgF, at 130,280 to 131,029
_04717
H281DRAFT_04718: H281DRAFT_04718 - peptidoglycan-associated lipoprotein, at 131,049 to 131,558
_04718
H281DRAFT_04719: H281DRAFT_04719 - TolB protein, at 131,590 to 132,882
_04719
H281DRAFT_04720: H281DRAFT_04720 - Cell division and transport-associated protein TolA, at 133,020 to 134,048
_04720
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 130.731 kb on + strand, within H281DRAFT_04717 at 130.735 kb on - strand, within H281DRAFT_04717 at 131.050 kb on + strand at 131.051 kb on - strand at 131.051 kb on - strand at 131.051 kb on - strand at 131.344 kb on + strand, within H281DRAFT_04718 at 131.345 kb on - strand, within H281DRAFT_04718 at 131.345 kb on - strand, within H281DRAFT_04718 at 131.592 kb on - strand at 131.624 kb on - strand at 131.810 kb on + strand, within H281DRAFT_04719 at 131.842 kb on - strand, within H281DRAFT_04719 at 131.951 kb on + strand, within H281DRAFT_04719 at 131.988 kb on - strand, within H281DRAFT_04719 at 132.216 kb on - strand, within H281DRAFT_04719 at 132.248 kb on + strand, within H281DRAFT_04719 at 132.381 kb on - strand, within H281DRAFT_04719 at 132.428 kb on + strand, within H281DRAFT_04719 at 132.430 kb on + strand, within H281DRAFT_04719 at 132.430 kb on + strand, within H281DRAFT_04719 at 132.431 kb on - strand, within H281DRAFT_04719 at 132.888 kb on - strand at 132.914 kb on + strand at 132.951 kb on - strand at 133.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 130,731 + H281DRAFT_04717 0.60 -1.4 130,735 - H281DRAFT_04717 0.61 -0.6 131,050 + -1.9 131,051 - +0.5 131,051 - +0.1 131,051 - +0.0 131,344 + H281DRAFT_04718 0.58 -0.5 131,345 - H281DRAFT_04718 0.58 -1.7 131,345 - H281DRAFT_04718 0.58 +1.1 131,592 - -0.4 131,624 - -1.3 131,810 + H281DRAFT_04719 0.17 -2.7 131,842 - H281DRAFT_04719 0.19 +0.1 131,951 + H281DRAFT_04719 0.28 +1.1 131,988 - H281DRAFT_04719 0.31 -2.3 132,216 - H281DRAFT_04719 0.48 -0.6 132,248 + H281DRAFT_04719 0.51 -1.5 132,381 - H281DRAFT_04719 0.61 -2.9 132,428 + H281DRAFT_04719 0.65 -1.7 132,430 + H281DRAFT_04719 0.65 -2.7 132,430 + H281DRAFT_04719 0.65 -1.9 132,431 - H281DRAFT_04719 0.65 -2.1 132,888 - +0.3 132,914 + -0.9 132,951 - +0.4 133,005 - -0.4
Or see this region's nucleotide sequence