Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04480 and H281DRAFT_04481 are separated by 73 nucleotides H281DRAFT_04481 and H281DRAFT_04482 overlap by 4 nucleotides H281DRAFT_04482 and H281DRAFT_04483 are separated by 167 nucleotides
H281DRAFT_04480: H281DRAFT_04480 - FAD/FMN-containing dehydrogenase, at 211,153 to 212,577
_04480
H281DRAFT_04481: H281DRAFT_04481 - Uncharacterized membrane protein, at 212,651 to 213,058
_04481
H281DRAFT_04482: H281DRAFT_04482 - NAD(P)H dehydrogenase (quinone), at 213,055 to 213,657
_04482
H281DRAFT_04483: H281DRAFT_04483 - tRNA-processing RNAse BN, at 213,825 to 215,135
_04483
Position (kb)
213
214 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 212.492 kb on + strand at 212.627 kb on + strand at 212.627 kb on + strand at 212.627 kb on + strand at 212.627 kb on + strand at 212.628 kb on - strand at 212.628 kb on - strand at 212.628 kb on - strand at 212.628 kb on - strand at 212.657 kb on - strand at 212.726 kb on - strand, within H281DRAFT_04481 at 212.726 kb on - strand, within H281DRAFT_04481 at 212.727 kb on + strand, within H281DRAFT_04481 at 212.728 kb on - strand, within H281DRAFT_04481 at 212.728 kb on - strand, within H281DRAFT_04481 at 212.760 kb on - strand, within H281DRAFT_04481 at 212.835 kb on + strand at 212.871 kb on + strand, within H281DRAFT_04481 at 212.872 kb on - strand, within H281DRAFT_04481 at 213.227 kb on + strand, within H281DRAFT_04482 at 213.392 kb on + strand at 213.392 kb on + strand, within H281DRAFT_04482 at 213.393 kb on - strand, within H281DRAFT_04482 at 213.632 kb on + strand at 213.633 kb on - strand at 213.633 kb on - strand at 213.635 kb on + strand at 213.635 kb on + strand at 213.636 kb on - strand at 213.658 kb on - strand at 213.678 kb on + strand at 213.679 kb on - strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.682 kb on - strand at 213.683 kb on + strand at 213.778 kb on + strand at 213.903 kb on - strand at 214.081 kb on - strand, within H281DRAFT_04483 at 214.300 kb on - strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.512 kb on + strand, within H281DRAFT_04483 at 214.516 kb on - strand, within H281DRAFT_04483 at 214.537 kb on - strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 212,492 + -3.3 212,627 + -0.3 212,627 + +0.2 212,627 + +0.8 212,627 + +0.4 212,628 - +0.3 212,628 - -0.1 212,628 - +0.0 212,628 - +1.0 212,657 - -1.4 212,726 - H281DRAFT_04481 0.18 -0.9 212,726 - H281DRAFT_04481 0.18 -0.9 212,727 + H281DRAFT_04481 0.19 -0.3 212,728 - H281DRAFT_04481 0.19 -0.2 212,728 - H281DRAFT_04481 0.19 -0.7 212,760 - H281DRAFT_04481 0.27 -0.4 212,835 + +0.6 212,871 + H281DRAFT_04481 0.54 -1.9 212,872 - H281DRAFT_04481 0.54 +0.2 213,227 + H281DRAFT_04482 0.29 -0.4 213,392 + -0.9 213,392 + H281DRAFT_04482 0.56 -0.2 213,393 - H281DRAFT_04482 0.56 +0.4 213,632 + -0.3 213,633 - +0.3 213,633 - +0.8 213,635 + -1.6 213,635 + +0.1 213,636 - -0.1 213,658 - +0.0 213,678 + +0.2 213,679 - -0.6 213,681 + +0.2 213,681 + +0.5 213,681 + -0.1 213,682 - -0.3 213,683 + +0.3 213,778 + -0.6 213,903 - +1.6 214,081 - H281DRAFT_04483 0.20 +0.4 214,300 - H281DRAFT_04483 0.36 +1.4 214,413 + H281DRAFT_04483 0.45 +0.5 214,413 + H281DRAFT_04483 0.45 +0.0 214,420 - H281DRAFT_04483 0.45 -0.4 214,420 - H281DRAFT_04483 0.45 +0.1 214,512 + H281DRAFT_04483 0.52 +0.2 214,516 - H281DRAFT_04483 0.53 +0.1 214,537 - H281DRAFT_04483 0.54 +0.4 214,590 + H281DRAFT_04483 0.58 +0.5 214,590 + H281DRAFT_04483 0.58 +0.7 214,591 - H281DRAFT_04483 0.58 +0.6 214,591 - H281DRAFT_04483 0.58 +0.4 214,591 - H281DRAFT_04483 0.58 -1.0
Or see this region's nucleotide sequence