Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04247 and H281DRAFT_04248 are separated by 19 nucleotides H281DRAFT_04248 and H281DRAFT_04249 are separated by 260 nucleotides
H281DRAFT_04247: H281DRAFT_04247 - aminopeptidase P Metallo peptidase. MEROPS family M24B, at 366,028 to 367,419
_04247
H281DRAFT_04248: H281DRAFT_04248 - 2-octaprenyl-6-methoxyphenol hydroxylase, at 367,439 to 368,809
_04248
H281DRAFT_04249: H281DRAFT_04249 - tRNA-U20-dihydrouridine synthase, at 369,070 to 370,146
_04249
Position (kb) 367
368
369 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 366.680 kb on - strand, within H281DRAFT_04247 at 366.739 kb on + strand, within H281DRAFT_04247 at 366.739 kb on + strand, within H281DRAFT_04247 at 366.740 kb on - strand, within H281DRAFT_04247 at 366.740 kb on - strand, within H281DRAFT_04247 at 366.741 kb on + strand, within H281DRAFT_04247 at 366.741 kb on + strand, within H281DRAFT_04247 at 366.741 kb on + strand, within H281DRAFT_04247 at 367.108 kb on + strand, within H281DRAFT_04247 at 367.108 kb on + strand, within H281DRAFT_04247 at 367.108 kb on + strand, within H281DRAFT_04247 at 367.108 kb on + strand, within H281DRAFT_04247 at 367.109 kb on - strand, within H281DRAFT_04247 at 367.110 kb on + strand, within H281DRAFT_04247 at 367.110 kb on + strand, within H281DRAFT_04247 at 367.111 kb on - strand, within H281DRAFT_04247 at 367.111 kb on - strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.255 kb on + strand, within H281DRAFT_04247 at 367.256 kb on - strand, within H281DRAFT_04247 at 367.256 kb on - strand, within H281DRAFT_04247 at 367.256 kb on - strand, within H281DRAFT_04247 at 368.814 kb on - strand at 368.838 kb on + strand at 368.839 kb on - strand at 368.933 kb on + strand at 368.934 kb on - strand at 368.995 kb on + strand at 368.996 kb on - strand at 369.096 kb on - strand at 369.102 kb on + strand at 369.103 kb on - strand at 369.178 kb on + strand, within H281DRAFT_04249 at 369.325 kb on + strand, within H281DRAFT_04249 at 369.326 kb on - strand, within H281DRAFT_04249 at 369.364 kb on - strand, within H281DRAFT_04249 at 369.364 kb on - strand, within H281DRAFT_04249 at 369.617 kb on - strand, within H281DRAFT_04249 at 369.634 kb on + strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 366,680 - H281DRAFT_04247 0.47 -0.9 366,739 + H281DRAFT_04247 0.51 +0.3 366,739 + H281DRAFT_04247 0.51 -0.6 366,740 - H281DRAFT_04247 0.51 -1.5 366,740 - H281DRAFT_04247 0.51 -0.9 366,741 + H281DRAFT_04247 0.51 +0.5 366,741 + H281DRAFT_04247 0.51 -0.5 366,741 + H281DRAFT_04247 0.51 +0.7 367,108 + H281DRAFT_04247 0.78 -0.2 367,108 + H281DRAFT_04247 0.78 -0.3 367,108 + H281DRAFT_04247 0.78 +0.6 367,108 + H281DRAFT_04247 0.78 +0.2 367,109 - H281DRAFT_04247 0.78 -0.9 367,110 + H281DRAFT_04247 0.78 -0.2 367,110 + H281DRAFT_04247 0.78 +0.0 367,111 - H281DRAFT_04247 0.78 +0.1 367,111 - H281DRAFT_04247 0.78 -2.0 367,255 + H281DRAFT_04247 0.88 +0.6 367,255 + H281DRAFT_04247 0.88 -0.5 367,255 + H281DRAFT_04247 0.88 -1.1 367,255 + H281DRAFT_04247 0.88 -1.7 367,255 + H281DRAFT_04247 0.88 +0.7 367,255 + H281DRAFT_04247 0.88 -0.5 367,255 + H281DRAFT_04247 0.88 +0.5 367,256 - H281DRAFT_04247 0.88 -0.2 367,256 - H281DRAFT_04247 0.88 +0.3 367,256 - H281DRAFT_04247 0.88 -1.1 368,814 - +0.6 368,838 + +0.1 368,839 - +0.2 368,933 + -0.5 368,934 - -0.4 368,995 + -0.0 368,996 - -0.6 369,096 - +0.1 369,102 + +0.2 369,103 - -0.0 369,178 + H281DRAFT_04249 0.10 -0.0 369,325 + H281DRAFT_04249 0.24 +0.5 369,326 - H281DRAFT_04249 0.24 -0.7 369,364 - H281DRAFT_04249 0.27 +1.0 369,364 - H281DRAFT_04249 0.27 -3.1 369,617 - H281DRAFT_04249 0.51 +0.0 369,634 + H281DRAFT_04249 0.52 +0.8 369,696 - H281DRAFT_04249 0.58 +1.1 369,696 - H281DRAFT_04249 0.58 +0.2
Or see this region's nucleotide sequence