Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04228 and H281DRAFT_04229 are separated by 165 nucleotides H281DRAFT_04229 and H281DRAFT_04230 are separated by 224 nucleotides
H281DRAFT_04228: H281DRAFT_04228 - homodimeric glycerol 3-phosphate dehydrogenase (quinone), at 347,106 to 348,635
_04228
H281DRAFT_04229: H281DRAFT_04229 - glycerol kinase, at 348,801 to 350,300
_04229
H281DRAFT_04230: H281DRAFT_04230 - glycerol uptake facilitator protein, at 350,525 to 351,238
_04230
Position (kb) 348
349
350
351 Strain fitness (log2 ratio) -2
-1
0
1
2 at 347.851 kb on - strand, within H281DRAFT_04228 at 347.851 kb on - strand, within H281DRAFT_04228 at 347.856 kb on + strand, within H281DRAFT_04228 at 347.856 kb on + strand, within H281DRAFT_04228 at 347.856 kb on + strand, within H281DRAFT_04228 at 347.857 kb on - strand, within H281DRAFT_04228 at 347.857 kb on - strand, within H281DRAFT_04228 at 347.857 kb on - strand, within H281DRAFT_04228 at 347.913 kb on + strand, within H281DRAFT_04228 at 348.058 kb on - strand, within H281DRAFT_04228 at 348.466 kb on - strand, within H281DRAFT_04228 at 348.466 kb on - strand, within H281DRAFT_04228 at 348.466 kb on - strand, within H281DRAFT_04228 at 348.484 kb on - strand at 348.767 kb on + strand at 348.779 kb on + strand at 348.780 kb on - strand at 348.813 kb on + strand at 348.813 kb on + strand at 348.813 kb on + strand at 348.813 kb on + strand at 348.813 kb on + strand at 348.814 kb on - strand at 348.814 kb on - strand at 348.915 kb on + strand at 349.037 kb on + strand, within H281DRAFT_04229 at 349.092 kb on + strand, within H281DRAFT_04229 at 349.092 kb on + strand, within H281DRAFT_04229 at 349.093 kb on - strand, within H281DRAFT_04229 at 349.101 kb on - strand, within H281DRAFT_04229 at 349.203 kb on + strand, within H281DRAFT_04229 at 349.470 kb on + strand, within H281DRAFT_04229 at 349.470 kb on + strand, within H281DRAFT_04229 at 349.470 kb on + strand, within H281DRAFT_04229 at 349.471 kb on - strand, within H281DRAFT_04229 at 349.471 kb on - strand, within H281DRAFT_04229 at 349.492 kb on + strand, within H281DRAFT_04229 at 349.492 kb on + strand, within H281DRAFT_04229 at 349.590 kb on + strand, within H281DRAFT_04229 at 349.590 kb on + strand, within H281DRAFT_04229 at 349.695 kb on + strand, within H281DRAFT_04229 at 349.695 kb on + strand, within H281DRAFT_04229 at 349.695 kb on + strand, within H281DRAFT_04229 at 349.696 kb on - strand, within H281DRAFT_04229 at 349.922 kb on + strand, within H281DRAFT_04229 at 350.348 kb on + strand at 350.348 kb on + strand at 350.498 kb on + strand at 350.534 kb on + strand at 350.681 kb on + strand, within H281DRAFT_04230 at 350.682 kb on - strand, within H281DRAFT_04230 at 350.682 kb on - strand, within H281DRAFT_04230 at 350.682 kb on - strand, within H281DRAFT_04230 at 350.696 kb on + strand, within H281DRAFT_04230 at 350.778 kb on - strand, within H281DRAFT_04230 at 350.834 kb on + strand, within H281DRAFT_04230 at 350.972 kb on + strand, within H281DRAFT_04230 at 350.973 kb on - strand, within H281DRAFT_04230 at 351.215 kb on + strand at 351.216 kb on - strand at 351.216 kb on - strand at 351.221 kb on + strand at 351.221 kb on + strand at 351.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 347,851 - H281DRAFT_04228 0.49 -0.2 347,851 - H281DRAFT_04228 0.49 +0.7 347,856 + H281DRAFT_04228 0.49 +0.7 347,856 + H281DRAFT_04228 0.49 -0.0 347,856 + H281DRAFT_04228 0.49 +0.7 347,857 - H281DRAFT_04228 0.49 +0.1 347,857 - H281DRAFT_04228 0.49 +0.8 347,857 - H281DRAFT_04228 0.49 +0.3 347,913 + H281DRAFT_04228 0.53 -0.2 348,058 - H281DRAFT_04228 0.62 +0.5 348,466 - H281DRAFT_04228 0.89 +0.5 348,466 - H281DRAFT_04228 0.89 +0.9 348,466 - H281DRAFT_04228 0.89 -0.2 348,484 - +0.4 348,767 + +0.7 348,779 + -0.1 348,780 - +0.2 348,813 + +0.3 348,813 + +0.4 348,813 + -0.2 348,813 + -0.2 348,813 + +0.7 348,814 - -0.5 348,814 - +0.4 348,915 + -0.2 349,037 + H281DRAFT_04229 0.16 -0.1 349,092 + H281DRAFT_04229 0.19 +0.7 349,092 + H281DRAFT_04229 0.19 -0.4 349,093 - H281DRAFT_04229 0.19 +0.1 349,101 - H281DRAFT_04229 0.20 +0.2 349,203 + H281DRAFT_04229 0.27 +1.0 349,470 + H281DRAFT_04229 0.45 +0.1 349,470 + H281DRAFT_04229 0.45 -0.2 349,470 + H281DRAFT_04229 0.45 +0.9 349,471 - H281DRAFT_04229 0.45 -0.5 349,471 - H281DRAFT_04229 0.45 +1.4 349,492 + H281DRAFT_04229 0.46 -0.3 349,492 + H281DRAFT_04229 0.46 -0.5 349,590 + H281DRAFT_04229 0.53 +0.6 349,590 + H281DRAFT_04229 0.53 -1.4 349,695 + H281DRAFT_04229 0.60 +0.4 349,695 + H281DRAFT_04229 0.60 -0.2 349,695 + H281DRAFT_04229 0.60 +1.1 349,696 - H281DRAFT_04229 0.60 +0.1 349,922 + H281DRAFT_04229 0.75 -0.3 350,348 + +0.2 350,348 + -0.8 350,498 + -1.1 350,534 + -0.6 350,681 + H281DRAFT_04230 0.22 -0.4 350,682 - H281DRAFT_04230 0.22 +0.0 350,682 - H281DRAFT_04230 0.22 -0.0 350,682 - H281DRAFT_04230 0.22 +0.3 350,696 + H281DRAFT_04230 0.24 +0.2 350,778 - H281DRAFT_04230 0.35 -0.3 350,834 + H281DRAFT_04230 0.43 -0.3 350,972 + H281DRAFT_04230 0.63 +0.2 350,973 - H281DRAFT_04230 0.63 -0.1 351,215 + +0.8 351,216 - +2.7 351,216 - -0.3 351,221 + -2.4 351,221 + +0.5 351,222 - -0.5
Or see this region's nucleotide sequence