Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04032 and H281DRAFT_04033 are separated by 197 nucleotides H281DRAFT_04033 and H281DRAFT_04034 are separated by 106 nucleotides H281DRAFT_04034 and H281DRAFT_04035 are separated by 108 nucleotides H281DRAFT_04035 and H281DRAFT_04036 are separated by 415 nucleotides
H281DRAFT_04032: H281DRAFT_04032 - conserved hypothetical protein, at 134,364 to 134,684
_04032
H281DRAFT_04033: H281DRAFT_04033 - YggT family protein, at 134,882 to 135,445
_04033
H281DRAFT_04034: H281DRAFT_04034 - tRNA_Gly_CCC, at 135,552 to 135,625
_04034
H281DRAFT_04035: H281DRAFT_04035 - hypothetical protein, at 135,734 to 136,117
_04035
H281DRAFT_04036: H281DRAFT_04036 - tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33), at 136,533 to 137,321
_04036
Position (kb) 135
136 Strain fitness (log2 ratio) -2
-1
0
1 at 134.578 kb on - strand, within H281DRAFT_04032 at 134.628 kb on + strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.800 kb on - strand at 134.800 kb on - strand at 134.870 kb on + strand at 134.870 kb on + strand at 134.870 kb on + strand at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.010 kb on - strand, within H281DRAFT_04033 at 135.431 kb on + strand at 135.431 kb on + strand at 135.431 kb on + strand at 135.432 kb on - strand at 135.490 kb on + strand at 135.542 kb on - strand at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.570 kb on + strand, within H281DRAFT_04034 at 135.571 kb on - strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.574 kb on - strand, within H281DRAFT_04034 at 135.579 kb on - strand, within H281DRAFT_04034 at 135.591 kb on + strand, within H281DRAFT_04034 at 135.628 kb on + strand at 135.628 kb on + strand at 135.678 kb on + strand at 135.678 kb on + strand at 135.679 kb on - strand at 135.731 kb on - strand at 135.787 kb on - strand, within H281DRAFT_04035 at 135.799 kb on + strand, within H281DRAFT_04035 at 135.801 kb on + strand, within H281DRAFT_04035 at 135.871 kb on - strand, within H281DRAFT_04035 at 136.034 kb on - strand, within H281DRAFT_04035 at 136.034 kb on - strand, within H281DRAFT_04035 at 136.055 kb on - strand, within H281DRAFT_04035 at 136.111 kb on + strand at 136.112 kb on - strand at 136.112 kb on - strand at 136.117 kb on + strand at 136.117 kb on + strand at 136.118 kb on - strand at 136.122 kb on + strand at 136.131 kb on - strand at 136.131 kb on - strand at 136.135 kb on + strand at 136.136 kb on - strand at 136.157 kb on + strand at 136.158 kb on - strand at 136.168 kb on + strand at 136.169 kb on - strand at 136.175 kb on + strand at 136.176 kb on - strand at 136.196 kb on - strand at 136.202 kb on + strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.268 kb on - strand at 136.268 kb on - strand at 136.322 kb on - strand at 136.343 kb on - strand at 136.371 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.520 kb on - strand at 136.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 134,578 - H281DRAFT_04032 0.67 -0.4 134,628 + H281DRAFT_04032 0.82 -0.5 134,629 - H281DRAFT_04032 0.83 -1.5 134,629 - H281DRAFT_04032 0.83 -1.9 134,629 - H281DRAFT_04032 0.83 -0.6 134,629 - H281DRAFT_04032 0.83 +1.1 134,800 - -0.3 134,800 - +1.3 134,870 + -0.3 134,870 + +1.7 134,870 + +0.1 135,009 + H281DRAFT_04033 0.23 -0.3 135,009 + H281DRAFT_04033 0.23 -0.9 135,009 + H281DRAFT_04033 0.23 -1.3 135,009 + H281DRAFT_04033 0.23 -0.6 135,010 - H281DRAFT_04033 0.23 -0.2 135,431 + -0.1 135,431 + -0.6 135,431 + -2.5 135,432 - -0.8 135,490 + +0.6 135,542 - +0.2 135,562 + H281DRAFT_04034 0.14 +0.5 135,562 + H281DRAFT_04034 0.14 +0.3 135,562 + H281DRAFT_04034 0.14 -0.7 135,565 - H281DRAFT_04034 0.18 -0.4 135,565 - H281DRAFT_04034 0.18 +0.6 135,570 + H281DRAFT_04034 0.24 +0.1 135,571 - H281DRAFT_04034 0.26 +0.5 135,573 + H281DRAFT_04034 0.28 -0.1 135,573 + H281DRAFT_04034 0.28 -1.1 135,573 + H281DRAFT_04034 0.28 -0.4 135,574 - H281DRAFT_04034 0.30 +1.0 135,579 - H281DRAFT_04034 0.36 +1.4 135,591 + H281DRAFT_04034 0.53 +0.0 135,628 + +0.4 135,628 + -0.5 135,678 + +1.3 135,678 + +0.4 135,679 - +1.2 135,731 - +0.7 135,787 - H281DRAFT_04035 0.14 -0.5 135,799 + H281DRAFT_04035 0.17 -0.8 135,801 + H281DRAFT_04035 0.17 -0.4 135,871 - H281DRAFT_04035 0.36 +0.6 136,034 - H281DRAFT_04035 0.78 -0.3 136,034 - H281DRAFT_04035 0.78 +1.2 136,055 - H281DRAFT_04035 0.84 -0.4 136,111 + +0.3 136,112 - -0.1 136,112 - +0.2 136,117 + +0.1 136,117 + +0.3 136,118 - +0.1 136,122 + -0.7 136,131 - +0.1 136,131 - -0.2 136,135 + -0.5 136,136 - +0.1 136,157 + +0.1 136,158 - -0.2 136,168 + +0.9 136,169 - +0.2 136,175 + -0.2 136,176 - -0.7 136,196 - +0.3 136,202 + -0.6 136,203 - +0.5 136,203 - -0.2 136,203 - -1.2 136,203 - -0.3 136,268 - +0.3 136,268 - +0.2 136,322 - -0.3 136,343 - +0.4 136,371 + -0.8 136,519 + +0.4 136,519 + +0.5 136,519 + -2.1 136,519 + -0.1 136,520 - +0.2 136,520 - +0.1
Or see this region's nucleotide sequence