Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03903 and H281DRAFT_03904 are separated by 237 nucleotides H281DRAFT_03904 and H281DRAFT_03905 are separated by 14 nucleotides H281DRAFT_03905 and H281DRAFT_03906 overlap by 11 nucleotides
H281DRAFT_03903: H281DRAFT_03903 - electron transfer flavoprotein beta subunit, at 3 to 653
_03903
H281DRAFT_03904: H281DRAFT_03904 - D-methionine transport system substrate-binding protein, at 891 to 1,688
_03904
H281DRAFT_03905: H281DRAFT_03905 - D-methionine transport system permease protein, at 1,703 to 2,356
_03905
H281DRAFT_03906: H281DRAFT_03906 - D-methionine transport system ATP-binding protein, at 2,346 to 3,380
_03906
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-1
0
1
2 at 0.698 kb on + strand at 0.699 kb on - strand at 0.736 kb on - strand at 0.835 kb on + strand at 0.835 kb on + strand at 0.836 kb on - strand at 0.836 kb on - strand at 0.852 kb on + strand at 0.989 kb on + strand, within H281DRAFT_03904 at 0.989 kb on + strand, within H281DRAFT_03904 at 1.027 kb on + strand, within H281DRAFT_03904 at 1.027 kb on + strand, within H281DRAFT_03904 at 1.027 kb on + strand, within H281DRAFT_03904 at 1.027 kb on + strand, within H281DRAFT_03904 at 1.028 kb on - strand, within H281DRAFT_03904 at 1.028 kb on - strand, within H281DRAFT_03904 at 1.169 kb on + strand, within H281DRAFT_03904 at 1.169 kb on + strand, within H281DRAFT_03904 at 1.170 kb on - strand, within H281DRAFT_03904 at 1.338 kb on + strand, within H281DRAFT_03904 at 1.351 kb on + strand, within H281DRAFT_03904 at 1.352 kb on - strand, within H281DRAFT_03904 at 1.352 kb on - strand, within H281DRAFT_03904 at 1.352 kb on - strand, within H281DRAFT_03904 at 1.429 kb on + strand, within H281DRAFT_03904 at 1.429 kb on + strand, within H281DRAFT_03904 at 1.445 kb on - strand, within H281DRAFT_03904 at 1.445 kb on - strand, within H281DRAFT_03904 at 1.642 kb on - strand at 1.705 kb on - strand at 1.708 kb on + strand at 1.709 kb on - strand at 1.709 kb on - strand at 1.878 kb on + strand, within H281DRAFT_03905 at 1.956 kb on - strand, within H281DRAFT_03905 at 1.956 kb on - strand, within H281DRAFT_03905 at 1.956 kb on - strand, within H281DRAFT_03905 at 2.162 kb on - strand, within H281DRAFT_03905 at 2.241 kb on + strand, within H281DRAFT_03905 at 2.242 kb on - strand, within H281DRAFT_03905 at 2.336 kb on - strand at 2.336 kb on - strand at 2.356 kb on + strand at 2.356 kb on + strand at 2.357 kb on - strand at 2.357 kb on - strand at 2.357 kb on - strand at 2.357 kb on - strand at 2.395 kb on + strand at 2.395 kb on + strand at 2.395 kb on + strand at 2.395 kb on + strand at 2.395 kb on + strand at 2.512 kb on + strand, within H281DRAFT_03906
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 698 + -0.2 699 - +0.8 736 - -0.1 835 + +0.1 835 + +0.5 836 - -1.2 836 - +0.1 852 + +0.2 989 + H281DRAFT_03904 0.12 +0.3 989 + H281DRAFT_03904 0.12 -0.5 1,027 + H281DRAFT_03904 0.17 +0.3 1,027 + H281DRAFT_03904 0.17 -0.1 1,027 + H281DRAFT_03904 0.17 +0.3 1,027 + H281DRAFT_03904 0.17 +0.0 1,028 - H281DRAFT_03904 0.17 -0.5 1,028 - H281DRAFT_03904 0.17 +0.3 1,169 + H281DRAFT_03904 0.35 +0.2 1,169 + H281DRAFT_03904 0.35 -1.1 1,170 - H281DRAFT_03904 0.35 +0.1 1,338 + H281DRAFT_03904 0.56 +0.2 1,351 + H281DRAFT_03904 0.58 -0.2 1,352 - H281DRAFT_03904 0.58 +0.7 1,352 - H281DRAFT_03904 0.58 -1.3 1,352 - H281DRAFT_03904 0.58 +1.4 1,429 + H281DRAFT_03904 0.67 -1.4 1,429 + H281DRAFT_03904 0.67 +1.9 1,445 - H281DRAFT_03904 0.69 -0.8 1,445 - H281DRAFT_03904 0.69 -0.2 1,642 - -0.4 1,705 - -0.2 1,708 + -0.2 1,709 - +0.2 1,709 - -0.9 1,878 + H281DRAFT_03905 0.27 +0.3 1,956 - H281DRAFT_03905 0.39 +0.7 1,956 - H281DRAFT_03905 0.39 -1.3 1,956 - H281DRAFT_03905 0.39 -0.1 2,162 - H281DRAFT_03905 0.70 +2.4 2,241 + H281DRAFT_03905 0.82 +1.8 2,242 - H281DRAFT_03905 0.82 -0.4 2,336 - -1.5 2,336 - +1.9 2,356 + -0.4 2,356 + +1.1 2,357 - -0.2 2,357 - -0.4 2,357 - +1.0 2,357 - +0.4 2,395 + -0.3 2,395 + -0.1 2,395 + -0.1 2,395 + +0.1 2,395 + -0.4 2,512 + H281DRAFT_03906 0.16 +0.4
Or see this region's nucleotide sequence